P08298 · UREA_SOYBN

Function

function

Catalyzes the conversion of urea to ammonia and carbon dioxide, thus allowing organisms to use exogenous and internally generated urea as a nitrogen source (Probable). May be involved in plant defense to pathogenic fungi (PubMed:22382992).

Miscellaneous

Plants with decreased expression of EU4 exhibit increased susceptibility to the fungal pathogen Phakopsora pachyrhizi.

Catalytic activity

Cofactor

Ni2+ (UniProtKB | Rhea| CHEBI:49786 )

Note: Binds 2 nickel ions per subunit.

Pathway

Nitrogen metabolism; urea degradation; CO2 and NH3 from urea (urease route): step 1/1.

Features

Showing features for binding site, active site.

TypeIDPosition(s)Description
Binding site405Ni2+ 1 (UniProtKB | ChEBI)
Binding site407Ni2+ 1 (UniProtKB | ChEBI)
Binding site488Ni2+ 1 (UniProtKB | ChEBI); via carbamate group
Binding site488Ni2+ 2 (UniProtKB | ChEBI); via carbamate group
Binding site490substrate
Binding site517Ni2+ 2 (UniProtKB | ChEBI)
Binding site543Ni2+ 2 (UniProtKB | ChEBI)
Active site591Proton donor
Binding site631Ni2+ 1 (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componenturease complex
Molecular Functionnickel cation binding
Molecular Functionurease activity
Biological Processurea catabolic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Urease
  • EC number
  • Alternative names
    • Ubiquitous urease
    • Urea amidohydrolase

Gene names

    • Name
      EU4
    • ORF names
      GLYMA_11G248700

Organism names

  • Taxonomic identifier
  • Strain
    • cv. Williams 82
  • Taxonomic lineage
    Eukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > fabids > Fabales > Fabaceae > Papilionoideae > 50 kb inversion clade > NPAAA clade > indigoferoid/millettioid clade > Phaseoleae > Glycine > Glycine subgen. Soja

Accessions

  • Primary accession
    P08298
  • Secondary accessions
    • Q949H4

Proteomes

Genome annotation databases

Subcellular Location

PTM/Processing

Features

Showing features for chain, modified residue.

TypeIDPosition(s)Description
ChainPRO_00000675251-837Urease
Modified residue488N6-carboxylysine

Post-translational modification

Carboxylation allows a single lysine to coordinate two nickel ions.

Proteomic databases

Expression

Induction

Induced by the fungal pathogen Phakopsora pachyrhizi 1 hour after pathogen inoculation, followed by a strong down-regulation at 24 hours and another up-regulation peak at 192 hours.

Interaction

Subunit

Homohexamer. Other oligomeric forms may exist depending on pH and presence of salts.

Protein-protein interaction databases

Chemistry

Structure

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain400-837Urease

Sequence similarities

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    837
  • Mass (Da)
    90,710
  • Last updated
    2022-12-14 v2
  • Checksum
    E157D512AB158B2B
MKLSPREIEKLDLHNAGYLAQKRLARGLRLNYVETVALIATQILEFVRDGEKTVAQLMCIGRELLGRKQVLPAVPHLVESVQVEATFRDGTKLVTIHDLFACENGNLELALFGSFLPVPSLDKFTENEEDHRTPGEIICRSENLILNPRRNAIILRVVNKGDRPIQVGSHYHFIEVNPYLTFDRRKAYGMRLNIAAGNATRFEPGECKSVVLVSIGGNKVIRGGNNIADGPVNDSNCRAAMKAVVTRGFGHVEEENAREGVTGEDYSLTTVISREEYAHKYGPTTGDKIRLGDTDLFAEIEKDFAVYGDECVFGGGKVIRDGMGQSSGHPPEGSLDTVITNAVIIDYTGIIKADIGIKDGLIISTGKAGNPDIMNDVFPNMIIGANTEVIAGEGLIVTAGAIDCHVHFICPQLVYDAVTSGITTLVGGGTGPADGTRATTCTPAPNQMKLMLQSTDDMPLNFGFTGKGNSAKPDELHEIIRAGAMGLKLHEDWGTTPAAIDSCLTVADQYDIQVNIHTDTLNESGFVEHTIAAFKGRTIHTYHSEGAGGGHAPDIIKVCGEKNVLPSSTNPTRPYTHNTIDEHLDMLMVCHHLNKNIPEDVAFAESRIRAETIAAEDILHDKGAISIISSDSQAMGRIGEVISRTWQTADKMKSQRGPLQPGEDNDNFRIKRYVAKYTINPAIANGLSQYVGSVEAGKLADLVLWKPSFFGAKPEMVIKGGEVAYANMGDPNASIPTPEPVIMRPMFGAFGKAGSSHSIAFVSKAALDEGVKASYGLNKRVEAVKNVRKLTKRDMKLNDTLPQITVDPETYTVTADGEVLTCTAAKTVPLSRNYFLF

Computationally mapped potential isoform sequences

There is 1 potential isoform mapped to this entry

View all
EntryEntry nameGene nameLength
A0A0R0HLW6A0A0R0HLW6_SOYBN547672643

Features

Showing features for sequence conflict.

TypeIDPosition(s)Description
Sequence conflict687in Ref. 4; AAA34018
Sequence conflict749-750in Ref. 4; AAA34018
Sequence conflict757in Ref. 4; AAA34018
Sequence conflict766-769in Ref. 4; AAA34018

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AJ276866
EMBL· GenBank· DDBJ
CAC43845.1
EMBL· GenBank· DDBJ
mRNA
AY230156
EMBL· GenBank· DDBJ
AAO85883.1
EMBL· GenBank· DDBJ
mRNA
CM000844
EMBL· GenBank· DDBJ
KRH31452.1
EMBL· GenBank· DDBJ
Genomic DNA
CM000844
EMBL· GenBank· DDBJ
KRH31451.1
EMBL· GenBank· DDBJ
Genomic DNA
M16772
EMBL· GenBank· DDBJ
AAA34018.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.
FeedbackHelp