P08298 · UREA_SOYBN
- ProteinUrease
- GeneEU4
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids837 (go to sequence)
- Protein existenceEvidence at transcript level
- Annotation score4/5
Function
function
Catalyzes the conversion of urea to ammonia and carbon dioxide, thus allowing organisms to use exogenous and internally generated urea as a nitrogen source (Probable). May be involved in plant defense to pathogenic fungi (PubMed:22382992).
Miscellaneous
Plants with decreased expression of EU4 exhibit increased susceptibility to the fungal pathogen Phakopsora pachyrhizi.
Catalytic activity
- 2 H+ + H2O + urea = CO2 + 2 NH4+
Cofactor
Note: Binds 2 nickel ions per subunit.
Pathway
Nitrogen metabolism; urea degradation; CO2 and NH3 from urea (urease route): step 1/1.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 405 | Ni2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Binding site | 407 | Ni2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Binding site | 488 | Ni2+ 1 (UniProtKB | ChEBI); via carbamate group | ||||
Sequence: K | ||||||
Binding site | 488 | Ni2+ 2 (UniProtKB | ChEBI); via carbamate group | ||||
Sequence: K | ||||||
Binding site | 490 | substrate | ||||
Sequence: H | ||||||
Binding site | 517 | Ni2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Binding site | 543 | Ni2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Active site | 591 | Proton donor | ||||
Sequence: H | ||||||
Binding site | 631 | Ni2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: D |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | urease complex | |
Molecular Function | nickel cation binding | |
Molecular Function | urease activity | |
Biological Process | urea catabolic process |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameUrease
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > fabids > Fabales > Fabaceae > Papilionoideae > 50 kb inversion clade > NPAAA clade > indigoferoid/millettioid clade > Phaseoleae > Glycine > Glycine subgen. Soja
Accessions
- Primary accessionP08298
- Secondary accessions
Proteomes
Genome annotation databases
Subcellular Location
UniProt Annotation
GO Annotation
PTM/Processing
Features
Showing features for chain, modified residue.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000067525 | 1-837 | Urease | |||
Sequence: MKLSPREIEKLDLHNAGYLAQKRLARGLRLNYVETVALIATQILEFVRDGEKTVAQLMCIGRELLGRKQVLPAVPHLVESVQVEATFRDGTKLVTIHDLFACENGNLELALFGSFLPVPSLDKFTENEEDHRTPGEIICRSENLILNPRRNAIILRVVNKGDRPIQVGSHYHFIEVNPYLTFDRRKAYGMRLNIAAGNATRFEPGECKSVVLVSIGGNKVIRGGNNIADGPVNDSNCRAAMKAVVTRGFGHVEEENAREGVTGEDYSLTTVISREEYAHKYGPTTGDKIRLGDTDLFAEIEKDFAVYGDECVFGGGKVIRDGMGQSSGHPPEGSLDTVITNAVIIDYTGIIKADIGIKDGLIISTGKAGNPDIMNDVFPNMIIGANTEVIAGEGLIVTAGAIDCHVHFICPQLVYDAVTSGITTLVGGGTGPADGTRATTCTPAPNQMKLMLQSTDDMPLNFGFTGKGNSAKPDELHEIIRAGAMGLKLHEDWGTTPAAIDSCLTVADQYDIQVNIHTDTLNESGFVEHTIAAFKGRTIHTYHSEGAGGGHAPDIIKVCGEKNVLPSSTNPTRPYTHNTIDEHLDMLMVCHHLNKNIPEDVAFAESRIRAETIAAEDILHDKGAISIISSDSQAMGRIGEVISRTWQTADKMKSQRGPLQPGEDNDNFRIKRYVAKYTINPAIANGLSQYVGSVEAGKLADLVLWKPSFFGAKPEMVIKGGEVAYANMGDPNASIPTPEPVIMRPMFGAFGKAGSSHSIAFVSKAALDEGVKASYGLNKRVEAVKNVRKLTKRDMKLNDTLPQITVDPETYTVTADGEVLTCTAAKTVPLSRNYFLF | ||||||
Modified residue | 488 | N6-carboxylysine | ||||
Sequence: K |
Post-translational modification
Carboxylation allows a single lysine to coordinate two nickel ions.
Proteomic databases
Expression
Induction
Induced by the fungal pathogen Phakopsora pachyrhizi 1 hour after pathogen inoculation, followed by a strong down-regulation at 24 hours and another up-regulation peak at 192 hours.
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 400-837 | Urease | ||||
Sequence: GAIDCHVHFICPQLVYDAVTSGITTLVGGGTGPADGTRATTCTPAPNQMKLMLQSTDDMPLNFGFTGKGNSAKPDELHEIIRAGAMGLKLHEDWGTTPAAIDSCLTVADQYDIQVNIHTDTLNESGFVEHTIAAFKGRTIHTYHSEGAGGGHAPDIIKVCGEKNVLPSSTNPTRPYTHNTIDEHLDMLMVCHHLNKNIPEDVAFAESRIRAETIAAEDILHDKGAISIISSDSQAMGRIGEVISRTWQTADKMKSQRGPLQPGEDNDNFRIKRYVAKYTINPAIANGLSQYVGSVEAGKLADLVLWKPSFFGAKPEMVIKGGEVAYANMGDPNASIPTPEPVIMRPMFGAFGKAGSSHSIAFVSKAALDEGVKASYGLNKRVEAVKNVRKLTKRDMKLNDTLPQITVDPETYTVTADGEVLTCTAAKTVPLSRNYFLF |
Sequence similarities
Belongs to the metallo-dependent hydrolases superfamily. Urease alpha subunit family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length837
- Mass (Da)90,710
- Last updated2022-12-14 v2
- ChecksumE157D512AB158B2B
Computationally mapped potential isoform sequences
There is 1 potential isoform mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
A0A0R0HLW6 | A0A0R0HLW6_SOYBN | 547672 | 643 |
Features
Showing features for sequence conflict.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Sequence conflict | 687 | in Ref. 4; AAA34018 | ||||
Sequence: L → F | ||||||
Sequence conflict | 749-750 | in Ref. 4; AAA34018 | ||||
Sequence: AF → TL | ||||||
Sequence conflict | 757 | in Ref. 4; AAA34018 | ||||
Sequence: H → L | ||||||
Sequence conflict | 766-769 | in Ref. 4; AAA34018 | ||||
Sequence: ALDE → WLNQ |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AJ276866 EMBL· GenBank· DDBJ | CAC43845.1 EMBL· GenBank· DDBJ | mRNA | ||
AY230156 EMBL· GenBank· DDBJ | AAO85883.1 EMBL· GenBank· DDBJ | mRNA | ||
CM000844 EMBL· GenBank· DDBJ | KRH31452.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CM000844 EMBL· GenBank· DDBJ | KRH31451.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
M16772 EMBL· GenBank· DDBJ | AAA34018.1 EMBL· GenBank· DDBJ | Genomic DNA |