P08018 · PBS2_YEAST

Function

function

Kinase involved in a signal transduction pathway that is activated by changes in the osmolarity of the extracellular environment (PubMed:10970855, PubMed:15256499, PubMed:7681220).
Activates the MAP kinase HOG1 by concomitant phosphorylation at 'Thr-174' and 'Tyr-176' (PubMed:38270553, PubMed:7681220).

Miscellaneous

Present with 2160 molecules/cell in log phase SD medium.

Catalytic activity

Features

Showing features for binding site, active site.

TypeIDPosition(s)Description
Binding site366-374ATP (UniProtKB | ChEBI)
Binding site389ATP (UniProtKB | ChEBI)
Active site485Proton acceptor

GO annotations

AspectTerm
Cellular Componentcellular bud neck
Cellular Componentcellular bud tip
Cellular Componentcytoplasm
Cellular Componentcytoplasmic stress granule
Cellular ComponentNatB complex
Molecular FunctionATP binding
Molecular FunctionMAP kinase kinase activity
Molecular FunctionMAP-kinase scaffold activity
Molecular Functionpeptide alpha-N-acetyltransferase activity
Molecular Functionprotein kinase activity
Molecular Functionprotein serine kinase activity
Molecular Functionprotein serine/threonine kinase activity
Molecular Functionprotein tyrosine kinase activity
Biological Processactin filament organization
Biological Processcellular hyperosmotic response
Biological Processhyperosmotic response
Biological ProcessMAPK cascade
Biological Processosmosensory signaling pathway
Biological Processosmosensory signaling pathway via Sho1 osmosensor
Biological Processp38MAPK cascade
Biological Processprotein import into nucleus
Biological Processprotein phosphorylation
Biological Processresponse to antibiotic

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    MAP kinase kinase PBS2
  • EC number
  • Alternative names
    • Polymyxin B resistance protein 2
    • Suppressor of fluoride sensitivity 4

Gene names

    • Name
      PBS2
    • Synonyms
      HOG4, SFS4, SSK4
    • ORF names
      J0699
    • Ordered locus names
      YJL128C

Organism names

Accessions

  • Primary accession
    P08018
  • Secondary accessions
    • D6VW57

Proteomes

Organism-specific databases

Subcellular Location

Phenotypes & Variants

Disruption phenotype

Leads to sensitivity to osmotic stress (induced by NaCl, KCl, and sorbitol), actinomycin D (transcription inhibitor), galactose, sirolimus (rapamycin), UV irradiation, and methyl methanesulfonate (MMS).

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis96In PBS2-13; loss of SH3-domain interaction.
Mutagenesis389Loss of activity.
Mutagenesis514Loss of phosphorylation.
Mutagenesis518Loss of phosphorylation.

Variants

We now provide the "Disease & Variants" viewer in its own tab.

The viewer provides 6 variants from UniProt as well as other sources including ClinVar and dbSNP.

Go to variant viewer

PTM/Processing

Features

Showing features for chain, modified residue.

TypeIDPosition(s)Description
ChainPRO_00000864831-668MAP kinase kinase PBS2
Modified residue68Phosphoserine
Modified residue269Phosphoserine
Modified residue514Phosphoserine
Modified residue518Phosphothreonine

Post-translational modification

Activated by phosphorylation by SSK2 or SSK22. Ser/Thr phosphorylation is also necessary for SHO1-mediated activation.

Keywords

Proteomic databases

PTM databases

Interaction

Subunit

Interacts with NBP2, PTC1, SHO1 and STE11.

Binary interactions

TypeEntry 1Entry 2Number of experimentsIntact
BINARY P08018NBP2 Q121633EBI-12972, EBI-34713
BINARY P08018SHO1 P400738EBI-12972, EBI-18140

Protein-protein interaction databases

Miscellaneous

Family & Domains

Features

Showing features for region, compositional bias, domain.

TypeIDPosition(s)Description
Region1-43Disordered
Compositional bias11-43Polar residues
Region61-120Disordered
Compositional bias64-89Polar residues
Compositional bias104-120Polar residues
Region181-313Disordered
Compositional bias234-313Polar residues
Domain360-623Protein kinase

Domain

Alternative way of activation involves binding the proline-rich motif to the SH3 domain of SHO1.

Sequence similarities

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    668
  • Mass (Da)
    72,720
  • Last updated
    2011-09-21 v4
  • Checksum
    9BC3435BDAFE8019
MEDKFANLSLHEKTGKSSIQLNEQTGSDNGSAVKRTSSTSSHYNNINADLHARVKAFQEQRALKRSASVGSNQSEQDKGSSQSPKHIQQIVNKPLPPLPVAGSSKVSQRMSSQVVQASSKSTLKNVLDNQETQNITDVNINIDTTKITATTIGVNTGLPATDITPSVSNTASATHKAQLLNPNRRAPRRPLSTQHPTRPNVAPHKAPAIINTPKQSLSARRGLKLPPGGMSLKMPTKTAQQPQQFAPSPSNKKHIETLSNSKVVEGKRSNPGSLINGVQSTSTSSSTEGPHDTVGTTPRTGNSNNSSNSGSSGGGGLFANFSKYVDIKSGSLNFAGKLSLSSKGIDFSNGSSSRITLDELEFLDELGHGNYGNVSKVLHKPTNVIMATKEVRLELDEAKFRQILMELEVLHKCNSPYIVDFYGAFFIEGAVYMCMEYMDGGSLDKIYDESSEIGGIDEPQLAFIANAVIHGLKELKEQHNIIHRDVKPTNILCSANQGTVKLCDFGVSGNLVASLAKTNIGCQSYMAPERIKSLNPDRATYTVQSDIWSLGLSILEMALGRYPYPPETYDNIFSQLSAIVDGPPPRLPSDKFSSDAQDFVSLCLQKIPERRPTYAALTEHPWLVKYRNQDVHMSEYITERLERRNKILRERGENGLSKNVPALHMGGL

Features

Showing features for compositional bias, sequence conflict.

TypeIDPosition(s)Description
Compositional bias11-43Polar residues
Compositional bias64-89Polar residues
Compositional bias104-120Polar residues
Sequence conflict222-223in Ref. 4 and 5; CAA89423
Compositional bias234-313Polar residues

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
J02946
EMBL· GenBank· DDBJ
AAA16819.1
EMBL· GenBank· DDBJ
Unassigned DNA
U12237
EMBL· GenBank· DDBJ
AAA20392.1
EMBL· GenBank· DDBJ
Genomic DNA
Z49403
EMBL· GenBank· DDBJ
CAA89423.1
EMBL· GenBank· DDBJ
Genomic DNA
BK006943
EMBL· GenBank· DDBJ
DAA08673.2
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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