P07984 · GUNA_CELFI
- ProteinEndoglucanase A
- GenecenA
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids449 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
The biological conversion of cellulose to glucose generally requires three types of hydrolytic enzymes: 1 Endoglucanases which cut internal beta-1,4-glucosidic bonds; 2 Exocellobiohydrolases that cut the disaccharide cellobiose from the non-reducing end of the cellulose polymer chain; 3 Beta-1,4-glucosidases which hydrolyze the cellobiose and other short cello-oligosaccharides to glucose.
Catalytic activity
Features
Showing features for active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Active site | 247 | |||||
Sequence: D | ||||||
Active site | 283 | Proton donor | ||||
Sequence: D | ||||||
Active site | 423 | Nucleophile | ||||
Sequence: D |
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Molecular Function | cellulase activity | |
Molecular Function | polysaccharide binding | |
Biological Process | cellulose catabolic process |
Keywords
- Molecular function
- Biological process
Enzyme and pathway databases
Protein family/group databases
Names & Taxonomy
Protein names
- Recommended nameEndoglucanase A
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Taxonomic lineageBacteria > Actinomycetota > Actinomycetes > Micrococcales > Cellulomonadaceae > Cellulomonas
Accessions
- Primary accessionP07984
PTM/Processing
Features
Showing features for signal, chain, disulfide bond.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Signal | 1-31 | Tat-type signal | ||||
Sequence: MSTRRTAAALLAAAAVAVGGLTALTTTAAQA | ||||||
Chain | PRO_0000007903 | 32-449 | Endoglucanase A | |||
Sequence: APGCRVDYAVTNQWPGGFGANVTITNLGDPVSSWKLDWTYTAGQRIQQLWNGTASTNGGQVSVTSLPWNGSIPTGGTASFGFNGSWAGSNPTPASFSLNGTTCTGTVPTTSPTPTPTPTTPTPTPTPTPTPTPTVTPQPTSGFYVDPTTQGYRAWQAASGTDKALLEKIALTPQAYWVGNWADASHAQAEVADYTGRAVAAGKTPMLVVYAIPGRDCGSHSGGGVSESEYARWVDTVAQGIKGNPIVILEPDALAQLGDCSGQGDRVGFLKYAAKSLTLKGARVYIDAGHAKWLSVDTPVNRLNQVGFEYAVGFALNTSNYQTTADSKAYGQQISQRLGGKKFVIDTSRNGNGSNGEWCNPRGRALGERPVAVNDGSGLDALLWVKLPGESDGACNGGPAAGQWWQEIALEMARNARW | ||||||
Disulfide bond | 35↔134 | |||||
Sequence: CRVDYAVTNQWPGGFGANVTITNLGDPVSSWKLDWTYTAGQRIQQLWNGTASTNGGQVSVTSLPWNGSIPTGGTASFGFNGSWAGSNPTPASFSLNGTTC | ||||||
Disulfide bond | 248↔291 | |||||
Sequence: CGSHSGGGVSESEYARWVDTVAQGIKGNPIVILEPDALAQLGDC | ||||||
Disulfide bond | 390↔426 | |||||
Sequence: CNPRGRALGERPVAVNDGSGLDALLWVKLPGESDGAC |
Post-translational modification
The linker region (also termed 'hinge') may be a potential site for proteolysis.
Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.
Keywords
- PTM
Structure
Family & Domains
Features
Showing features for domain, compositional bias, region.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 32-137 | CBM2 | ||||
Sequence: APGCRVDYAVTNQWPGGFGANVTITNLGDPVSSWKLDWTYTAGQRIQQLWNGTASTNGGQVSVTSLPWNGSIPTGGTASFGFNGSWAGSNPTPASFSLNGTTCTGT | ||||||
Compositional bias | 127-141 | Polar residues | ||||
Sequence: FSLNGTTCTGTVPTT | ||||||
Region | 127-170 | Disordered | ||||
Sequence: FSLNGTTCTGTVPTTSPTPTPTPTTPTPTPTPTPTPTPTVTPQP | ||||||
Region | 139-168 | Linker ('hinge') (Pro-Thr box) | ||||
Sequence: PTTSPTPTPTPTTPTPTPTPTPTPTPTVTP | ||||||
Compositional bias | 142-167 | Pro residues | ||||
Sequence: SPTPTPTPTTPTPTPTPTPTPTPTVT | ||||||
Region | 438-449 | Catalytic | ||||
Sequence: EIALEMARNARW |
Sequence similarities
Belongs to the glycosyl hydrolase 6 (cellulase B) family.
Keywords
- Domain
Family and domain databases
Sequence
- Sequence statusComplete
- Sequence processingThe displayed sequence is further processed into a mature form.
- Length449
- Mass (Da)46,731
- Last updated1988-08-01 v1
- Checksum67FF887814B3348D
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 127-141 | Polar residues | ||||
Sequence: FSLNGTTCTGTVPTT | ||||||
Compositional bias | 142-167 | Pro residues | ||||
Sequence: SPTPTPTPTTPTPTPTPTPTPTPTVT |