P07821 · FHUC_ECOLI
- ProteinIron(3+)-hydroxamate import ATP-binding protein FhuC
- GenefhuC
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids265 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Part of the ABC transporter complex FhuCDB involved in iron3+-hydroxamate import. Responsible for energy coupling to the transport system.
Catalytic activity
Activity regulation
ATPase activity is inhibited by vanadate.
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
0.69 mM | ATP using FhuCB complex | |||||
0.74 mM | ATP using FhuCB complex with enclosed FhuD | |||||
0.77 mM | ATP using FhuCB complex with enclosed ferrichrome-loaded FhuD |
Vmax | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|
745 nmol/min/mg | using FhuCB complex | ||||
127 nmol/min/mg | using FhuCB complex with enclosed FhuD | ||||
155 nmol/min/mg | using FhuCB complex with enclosed ferrichrome-loaded FhuD |
Features
Showing features for binding site.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | ATP-binding cassette (ABC) transporter complex | |
Cellular Component | ATP-binding cassette (ABC) transporter complex, substrate-binding subunit-containing | |
Cellular Component | plasma membrane | |
Molecular Function | ABC-type ferric hydroxamate transporter activity | |
Molecular Function | ATP binding | |
Molecular Function | ATP hydrolysis activity | |
Molecular Function | ferric iron transmembrane transporter activity | |
Molecular Function | siderophore uptake transmembrane transporter activity | |
Biological Process | ferric-hydroxamate import into cell | |
Biological Process | iron ion import across plasma membrane |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Protein family/group databases
Names & Taxonomy
Protein names
- Recommended nameIron(3+)-hydroxamate import ATP-binding protein FhuC
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strains
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Enterobacterales > Enterobacteriaceae > Escherichia
Accessions
- Primary accessionP07821
- Secondary accessions
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Cell inner membrane ; Peripheral membrane protein
Note: FhuB mediates the association of FhuC with the cytoplasmic membrane.
Keywords
- Cellular component
Phenotypes & Variants
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 50 | Lack of activity. | ||||
Sequence: K → Q | ||||||
Mutagenesis | 172 | Lack of activity. | ||||
Sequence: D → E | ||||||
Mutagenesis | 173 | Lack of activity. | ||||
Sequence: E → A |
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000092327 | 1-265 | Iron3+-hydroxamate import ATP-binding protein FhuC | |||
Sequence: MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIGHNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTVRELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAMLVAQDSRCLLLDEPTSALDIAHQVDVLSLVHRLSQERGLTVIAVLHDINMAARYCDYLVALRGGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY |
Proteomic databases
Expression
Induction
Induced 1.6-fold by hydroxyurea.
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 12-248 | ABC transporter | ||||
Sequence: FALRNISFRVPGRTLLHPLSLTFPAGKVTGLIGHNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTVRELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAMLVAQDSRCLLLDEPTSALDIAHQVDVLSLVHRLSQERGLTVIAVLHDINMAARYCDYLVALRGGEMIAQGTPAEIMRGETLEMIYGIP |
Sequence similarities
Belongs to the ABC transporter superfamily. Iron (Fe3+)-hydroxamate importer (TC 3.A.1.14.7) family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length265
- Mass (Da)28,886
- Last updated1997-11-01 v3
- Checksum2511770D6D9EE772
Features
Showing features for sequence conflict.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Sequence conflict | 31 | in Ref. 2; CAA29254 | ||||
Sequence: S → C | ||||||
Sequence conflict | 226-249 | in Ref. 1; AAB61769 and 3; no nucleotide entry | ||||
Sequence: MIAQGTPAEIMRGETLEMIYGIPM → SDCSGNACGNYARRNPRNDLWHPD |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
M12486 EMBL· GenBank· DDBJ | AAB61769.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
X05810 EMBL· GenBank· DDBJ | CAA29254.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
U70214 EMBL· GenBank· DDBJ | AAB08581.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
U00096 EMBL· GenBank· DDBJ | AAC73262.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AP009048 EMBL· GenBank· DDBJ | BAB96727.2 EMBL· GenBank· DDBJ | Genomic DNA |