P07329 · NIFK_AZOVI

  • Protein
    Nitrogenase molybdenum-iron protein beta chain
  • Gene
    nifK
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at protein level
  • Annotation score
    5/5

Function

function

This molybdenum-iron protein is part of the nitrogenase complex that catalyzes the key enzymatic reactions in nitrogen fixation.

Catalytic activity

Cofactor

[8Fe-7S] cluster (UniProtKB | Rhea| CHEBI:21143 )
Note: Binds 1 [8Fe-7S] cluster per heterodimer.

Activity regulation

Nitrogenase holoenzyme is subject to 'conformational protection' by FeSII; under oxidizing conditions FeSII binds to the holoenzyme and reversibly protects it from oxidation (PubMed:7830548).

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site70[8Fe-7S] cluster (UniProtKB | ChEBI); ligand shared with alpha chain
Binding site95[8Fe-7S] cluster (UniProtKB | ChEBI); ligand shared with alpha chain
Binding site153[8Fe-7S] cluster (UniProtKB | ChEBI); ligand shared with alpha chain
Binding site188[8Fe-7S] cluster (UniProtKB | ChEBI); ligand shared with alpha chain

GO annotations

AspectTerm
Cellular Componentmolybdenum-iron nitrogenase complex
Molecular FunctionATP binding
Molecular Functioncarbonyl sulfide nitrogenase activity
Molecular Functioniron-sulfur cluster binding
Molecular Functionmetal ion binding
Molecular Functionnitrogenase activity
Biological Processnitrogen fixation

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Nitrogenase molybdenum-iron protein beta chain
  • EC number
  • Alternative names
    • Dinitrogenase
    • Nitrogenase component I

Gene names

    • Name
      nifK

Organism names

  • Taxonomic identifier
  • Strain
    • ATCC 13705 / OP1 / DSM 366 / NCIMB 11614 / LMG 3878 / UW
  • Taxonomic lineage
    Bacteria > Pseudomonadota > Gammaproteobacteria > Pseudomonadales > Pseudomonadaceae > Azotobacter

Accessions

  • Primary accession
    P07329

Subcellular Location

PTM/Processing

Features

Showing features for initiator methionine, chain.

TypeIDPosition(s)Description
Initiator methionine1Removed
ChainPRO_00001530912-523Nitrogenase molybdenum-iron protein beta chain

Expression

Induction

Constitutively expressed during log and stationary phase in sucrose-limited cultures, its levels decrease during stationary phase (at protein level).

Interaction

Subunit

Tetramer of two alpha and two beta chains. Forms complex with the iron protein (nitrogenase component 2).

Family & Domains

Sequence similarities

Belongs to the NifD/NifK/NifE/NifN family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    523
  • Mass (Da)
    59,460
  • Last updated
    2007-01-23 v3
  • Checksum
    B6ECD633A24998F2
MSQQVDKIKASYPLFLDQDYKDMLAKKRDGFEEKYPQDKIDEVFQWTTTKEYQELNFQREALTVNPAKACQPLGAVLCALGFEKTMPYVHGSQGCVAYFRSYFNRHFREPVSCVSDSMTEDAAVFGGQQNMKDGLQNCKATYKPDMIAVSTTCMAEVIGDDLNAFINNSKKEGFIPDEFPVPFAHTPSFVGSHVTGWDNMFEGIARYFTLKSMDDKVVGSNKKINIVPGFETYLGNFRVIKRMLSEMGVGYSLLSDPEEVLDTPADGQFRMYAGGTTQEEMKDAPNALNTVLLQPWHLEKTKKFVEGTWKHEVPKLNIPMGLDWTDEFLMKVSEISGQPIPASLTKERGRLVDMMTDSHTWLHGKRFALWGDPDFVMGLVKFLLELGCEPVHILCHNGNKRWKKAVDAILAASPYGKNATVYIGKDLWHLRSLVFTDKPDFMIGNSYGKFIQRDTLHKGKEFEVPLIRIGFPIFDRHHLHRSTTLGYEGAMQILTTLVNSILERLDEETRGMQATDYNHDLVR

Features

Showing features for sequence conflict.

TypeIDPosition(s)Description
Sequence conflict103in Ref. 2; AAA22144

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
M20568
EMBL· GenBank· DDBJ
AAA64711.1
EMBL· GenBank· DDBJ
Genomic DNA
M11579
EMBL· GenBank· DDBJ
AAA22144.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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