P07244 · PUR2_YEAST

Function

function

Catalyzes the second and fifth step in the 'de novo' purine biosynthesis pathway; contains phosphoribosylamine--glycine ligase (GARS) and phosphoribosylformylglycinamidine cyclo-ligase (AIRS) activities.

Miscellaneous

Present with 35800 molecules/cell in log phase SD medium.

Catalytic activity

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Mn2+ (UniProtKB | Rhea| CHEBI:29035 )

Note: Binds two magnesium or manganese ions per subunit.

Pathway

Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N2-formyl-N1-(5-phospho-D-ribosyl)glycinamide: step 2/2.
Purine metabolism; IMP biosynthesis via de novo pathway; N1-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/2.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site141-203ATP (UniProtKB | ChEBI)
Binding site298Mg2+ (UniProtKB | ChEBI)
Binding site300Mg2+ (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytoplasm
Cellular Componentcytosol
Molecular FunctionATP binding
Molecular Functionmetal ion binding
Molecular Functionphosphoribosylamine-glycine ligase activity
Molecular Functionphosphoribosylformylglycinamidine cyclo-ligase activity
Biological Process'de novo' IMP biosynthetic process
Biological Processadenine biosynthetic process
Biological Processpurine nucleobase metabolic process
Biological Processpurine nucleotide biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Bifunctional purine biosynthetic protein ADE5,7

Including 2 domains:

  • Recommended name
    Phosphoribosylamine--glycine ligase
  • EC number
  • Alternative names
    • Glycinamide ribonucleotide synthetase (GAR synthetase
      ; GARS)
    • Phosphoribosylglycinamide synthetase
  • Recommended name
    Phosphoribosylformylglycinamidine cyclo-ligase
  • EC number
  • Alternative names
    • AIR synthase (AIR synthetase; AIRS)
    • Phosphoribosyl-aminoimidazole synthetase

Gene names

    • Name
      ADE57
    • Synonyms
      ADE5,7
    • Ordered locus names
      YGL234W

Organism names

Accessions

  • Primary accession
    P07244
  • Secondary accessions
    • D6VVA0
    • E9P907

Proteomes

Organism-specific databases

Subcellular Location

Keywords

PTM/Processing

Features

Showing features for chain, modified residue.

TypeIDPosition(s)Description
ChainPRO_00000749411-802Bifunctional purine biosynthetic protein ADE5,7
Modified residue455Phosphoserine
Modified residue458Phosphoserine

Keywords

Proteomic databases

PTM databases

Interaction

Protein-protein interaction databases

Miscellaneous

Structure

Family & Domains

Features

Showing features for region, domain.

TypeIDPosition(s)Description
Region1-450GARS
Domain114-330ATP-grasp
Region451-802AIRS

Sequence similarities

In the N-terminal section; belongs to the GARS family.
In the C-terminal section; belongs to the AIR synthase family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    802
  • Mass (Da)
    86,068
  • Last updated
    1988-04-01 v1
  • Checksum
    1583C6F3E64085D2
MLNILVLGNGAREHVLVTKLAQSPTVGKIYVAPGNGGTATMDPSRVINWDITPDVANFARLQSMAVEHKINLVVPGPELPLVNGITSVFHSVGIPVFGPSVKAAQLEASKAFSKRFMSKHNIPTASYDVFTNPEEAISFLQAHTDKAFVIKADGIAAGKGVIIPSSIDESVQAIKDIMVTKQFGEEAGKQVVIEQFLEGDEISLLTIVDGYSHFNLPVAQDHKRIFDGDKGLNTGGMGAYAPAPVATPSLLKTIDSQIVKPTIDGMRRDGMPFVGVLFTGMILVKDSKTNQLVPEVLEYNVRFGDPETQAVLSLLDDQTDLAQVFLAAAEHRLDSVNIGIDDTRSAVTVVVAAGGYPESYAKGDKITLDTDKLPPHTQIFQAGTKYDSATDSLLTNGGRVLSVTSTAQDLRTAVDTVYEAVKCVHFQNSYYRKDIAYRAFQNSESSKVAITYADSGVSVDNGNNLVQTIKEMVRSTRRPGADSDIGGFGGLFDLAQAGFRQNEDTLLVGATDGVGTKLIIAQETGIHNTVGIDLVAMNVNDLVVQGAEPLFFLDYFATGALDIQVASDFVSGVANGCIQSGCALVGGETSEMPGMYPPGHYDTNGTAVGAVLRQDILPKINEMAAGDVLLGLASSGVHSNGFSLVRKIIQHVALPWDAPCPWDESKTLGEGILEPTKIYVKQLLPSIRQRLLLGLAHITGGGLVENIPRAIPDHLQARVDMSTWEVPRVFKWFGQAGNVPHDDILRTFNMGVGMVLIVKRENVKAVCDSLTEEGEIIWELGSLQERPKDAPGCVIENGTKLY

Features

Showing features for sequence conflict.

TypeIDPosition(s)Description
Sequence conflict28in Ref. 4; AAT93005

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
X04337
EMBL· GenBank· DDBJ
CAA27867.1
EMBL· GenBank· DDBJ
Genomic DNA
Z72756
EMBL· GenBank· DDBJ
CAA96952.1
EMBL· GenBank· DDBJ
Genomic DNA
AY692986
EMBL· GenBank· DDBJ
AAT93005.1
EMBL· GenBank· DDBJ
Genomic DNA
BK006941
EMBL· GenBank· DDBJ
DAA07884.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

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