P07024 · USHA_ECOLI
- ProteinProtein UshA
- GeneushA
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids550 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Degradation of external UDP-glucose to uridine monophosphate and glucose-1-phosphate, which can then be used by the cell.
Catalytic activity
- a ribonucleoside 5'-phosphate + H2O = a ribonucleoside + phosphate
Cofactor
Note: Binds 2 Zn2+ ions per subunit.
Activity regulation
The activity of this protein is inhibited by an intracellular protein inhibitor.
Features
Showing features for binding site, site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 41 | Zn2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 43 | Zn2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Binding site | 84 | Zn2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 84 | Zn2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 116 | Zn2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: N | ||||||
Site | 117 | Transition state stabilizer | ||||
Sequence: H | ||||||
Site | 120 | Transition state stabilizer | ||||
Sequence: D | ||||||
Binding site | 217 | Zn2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Binding site | 252 | Zn2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Binding site | 254 | Zn2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: Q | ||||||
Binding site | 375-379 | substrate | ||||
Sequence: RDKVR | ||||||
Binding site | 498-504 | substrate | ||||
Sequence: FNATGGD |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | outer membrane-bounded periplasmic space | |
Molecular Function | 5'-nucleotidase activity | |
Molecular Function | metal ion binding | |
Molecular Function | nucleotide binding | |
Molecular Function | UDP-sugar diphosphatase activity | |
Molecular Function | XMP 5'-nucleosidase activity | |
Biological Process | nucleotide catabolic process |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameProtein UshA
Including 2 domains:
- Recommended nameUDP-sugar hydrolase
- EC number
- Alternative names
- Recommended name5'-nucleotidase
- EC number
- Short names5'-NT
Gene names
Organism names
- Organism
- Strains
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Enterobacterales > Enterobacteriaceae > Escherichia
Accessions
- Primary accessionP07024
- Secondary accessions
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Note: Exported from the cell, except a small proportion that is internally localized.
Keywords
- Cellular component
Phenotypes & Variants
Chemistry
PTM/Processing
Features
Showing features for signal, chain, disulfide bond.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Signal | 1-25 | |||||
Sequence: MKLLQRGVALALLTTFTLASETALA | ||||||
Chain | PRO_0000000031 | 26-550 | Protein UshA | |||
Sequence: YEQDKTYKITVLHTNDHHGHFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFRGMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKRQDLKIAVIGLTTDDTAKIGNPEYFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHMGHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCKPDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVLYTPEIAENQQMISLLSPFQNKGKAQLEVKIGETNGRLEGDRDKVRFVQTNMGRLILAAQMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAVAQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPRLDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ | ||||||
Disulfide bond | 258↔275 | |||||
Sequence: CMAAENKKQVDYVPGTPC |
Keywords
- PTM
Proteomic databases
Structure
Sequence
- Sequence statusComplete
- Sequence processingThe displayed sequence is further processed into a mature form.
- Length550
- Mass (Da)60,824
- Last updated1997-11-01 v2
- ChecksumDBA07B1C40C6C075
Features
Showing features for sequence conflict.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Sequence conflict | 256 | in Ref. 1; CAA27532 | ||||
Sequence: P → T |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
X03895 EMBL· GenBank· DDBJ | CAA27532.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
U82664 EMBL· GenBank· DDBJ | AAB40234.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
U00096 EMBL· GenBank· DDBJ | AAC73582.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AP009048 EMBL· GenBank· DDBJ | BAE76259.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
D73370 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. |