P06960 · OTC2_ECOLI
- ProteinOrnithine carbamoyltransferase subunit F
- GeneargF
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids334 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Reversibly catalyzes the transfer of the carbamoyl group from carbamoyl phosphate (CP) to the N(epsilon) atom of ornithine (ORN) to produce L-citrulline, which is a substrate for argininosuccinate synthetase, the enzyme involved in the final step in arginine biosynthesis.
Miscellaneous
ArgF might be the product of a relatively recent event (duplication or transposition) peculiar to E.coli strain K-12.
Catalytic activity
- carbamoyl phosphate + L-ornithine = H+ + L-citrulline + phosphate
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
0.02 mM | carbamoyl phosphate | |||||
5 mM | L-ornithine |
Pathway
Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 1/3.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 56-59 | carbamoyl phosphate (UniProtKB | ChEBI) | ||||
Sequence: STRT | ||||||
Binding site | 83 | carbamoyl phosphate (UniProtKB | ChEBI) | ||||
Sequence: Q | ||||||
Binding site | 107 | carbamoyl phosphate (UniProtKB | ChEBI) | ||||
Sequence: R | ||||||
Binding site | 134-137 | carbamoyl phosphate (UniProtKB | ChEBI) | ||||
Sequence: HPTQ | ||||||
Binding site | 168 | L-ornithine (UniProtKB | ChEBI) | ||||
Sequence: N | ||||||
Binding site | 232 | L-ornithine (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 236-237 | L-ornithine (UniProtKB | ChEBI) | ||||
Sequence: SM | ||||||
Binding site | 274-275 | carbamoyl phosphate (UniProtKB | ChEBI) | ||||
Sequence: CL | ||||||
Binding site | 320 | carbamoyl phosphate (UniProtKB | ChEBI) | ||||
Sequence: R |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | amino acid binding | |
Molecular Function | metal ion binding | |
Molecular Function | ornithine carbamoyltransferase activity | |
Biological Process | arginine biosynthetic process via ornithine | |
Biological Process | citrulline biosynthetic process | |
Biological Process | DNA damage response |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameOrnithine carbamoyltransferase subunit F
- EC number
- Short namesOTCase-2
Gene names
Organism names
- Organism
- Strains
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Enterobacterales > Enterobacteriaceae > Escherichia
Accessions
- Primary accessionP06960
- Secondary accessions
Proteomes
Subcellular Location
PTM/Processing
Features
Showing features for initiator methionine, chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Initiator methionine | 1 | Removed | ||||
Sequence: M | ||||||
Chain | PRO_0000112919 | 2-334 | Ornithine carbamoyltransferase subunit F | |||
Sequence: SDLYKKHFLKLLDFTPAQFTSLLTLAAQLKADKKNGKEVQKLTGKNIALIFEKDSTRTRCSFEVAAFDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYDGIQYRGHGQEVVETLAQYAGVPVWNGLTNEFHPTQLLADLMTMQEHLPGKAFNEMTLVYAGDARNNMGNSMLEAAALTGLDLRLLAPKACWPEESLVAECSALAEKHGGKITLTEDVAAGVKGADFIYTDVWVSMGEAKEKWAERIALLRGYQVNAQMMALTDNPNVKFLHCLPAFHDDQTTLGKQMAKEFDLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMMATLGE |
Proteomic databases
Interaction
Subunit
In E.coli strain K12, trimer of identical or non-identical chains are composed of ArgI (I) and/or ArgF (F). The trimer has the following composition: FFI, FFF, FII, III. E.coli strains B and W, which are known to contain only ArgI, produce only a trimer of identical chains (III).
Protein-protein interaction databases
Structure
Sequence
- Sequence statusComplete
- Length334
- Mass (Da)36,827
- Last updated2007-01-23 v4
- ChecksumC1EA07CD2AFC39C4
Features
Showing features for sequence conflict.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Sequence conflict | 121 | in Ref. 1; CAA25329 | ||||
Sequence: A → P |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
X00759 EMBL· GenBank· DDBJ | CAA25329.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
U70214 EMBL· GenBank· DDBJ | AAB08694.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
U00096 EMBL· GenBank· DDBJ | AAC73376.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AP009048 EMBL· GenBank· DDBJ | BAE76057.1 EMBL· GenBank· DDBJ | Genomic DNA |