P05532 · KIT_MOUSE
- ProteinMast/stem cell growth factor receptor Kit
- GeneKit
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids979 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Miscellaneous
Catalytic activity
- ATP + L-tyrosyl-[protein] = ADP + H+ + O-phospho-L-tyrosyl-[protein]
Activity regulation
Features
Showing features for binding site, active site, site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 571 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: Y | ||||||
Binding site | 599-606 | ATP (UniProtKB | ChEBI) | ||||
Sequence: GAGAFGKV | ||||||
Binding site | 626 | ATP (UniProtKB | ChEBI) | ||||
Sequence: K | ||||||
Binding site | 674-680 | ATP (UniProtKB | ChEBI) | ||||
Sequence: EYCCYGD | ||||||
Active site | 794 | Proton acceptor | ||||
Sequence: D | ||||||
Binding site | 798 | ATP (UniProtKB | ChEBI) | ||||
Sequence: R | ||||||
Binding site | 799 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: N | ||||||
Binding site | 812 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Site | 938 | Important for interaction with phosphotyrosine-binding proteins | ||||
Sequence: Y |
GO annotations
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameMast/stem cell growth factor receptor Kit
- EC number
- Short namesSCFR
- Alternative names
- CD Antigen Name
Gene names
Organism names
- Organism
- Strains
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Mus > Mus
Accessions
- Primary accessionP05532
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
Isoform 1
Isoform 2
Isoform 3
Features
Showing features for topological domain, transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Topological domain | 25-527 | Extracellular | ||||
Sequence: SQPSASPGEPSPPSIHPAQSELIVEAGDTLSLTCIDPDFVRWTFKTYFNEMVENKKNEWIQEKAEATRTGTYTCSNSNGLTSSIYVFVRDPAKLFLVGLPLFGKEDSDALVRCPLTDPQVSNYSLIECDGKSLPTDLTFVPNPKAGITIKNVKRAYHRLCVRCAAQRDGTWLHSDKFTLKVRAAIKAIPVVSVPETSHLLKKGDTFTVVCTIKDVSTSVNSMWLKMNPQPQHIAQVKHNSWHRGDFNYERQETLTISSARVDDSGVFMCYANNTFGSANVTTTLKVVEKGFINISPVKNTTVFVTDGENVDLVVEYEAYPKPEHQQWIYMNRTSANKGKDYVKSDNKSNIRYVNQLRLTRLKGTEGGTYTFLVSNSDASASVTFNVYVNTKPEILTYDRLINGMLQCVAEGFPEPTIDWYFCTGAEQRCTTPVSPVDVQVQNVSVSPFGKLVVQSSIDSSVFRHNGTVECKASNDVGKSSAFFNFAFKGNNKEQIQAHTLFTP | ||||||
Transmembrane | 528-548 | Helical | ||||
Sequence: LLIGFVVAAGAMGIIVMVLTY | ||||||
Topological domain | 549-979 | Cytoplasmic | ||||
Sequence: KYLQKPMYEVQWKVVEEINGNNYVYIDPTQLPYDHKWEFPRNRLSFGKTLGAGAFGKVVEATAYGLIKSDAAMTVAVKMLKPSAHLTEREALMSELKVLSYLGNHMNIVNLLGACTVGGPTLVITEYCCYGDLLNFLRRKRDSFIFSKQEEQAEAALYKNLLHSTEPSCDSSNEYMDMKPGVSYVVPTKTDKRRSARIDSYIERDVTPAIMEDDELALDLDDLLSFSYQVAKGMAFLASKNCIHRDLAARNILLTHGRITKICDFGLARDIRNDSNYVVKGNARLPVKWMAPESIFSCVYTFESDVWSYGIFLWELFSLGSSPYPGMPVDSKFYKMIKEGFRMVSPEHAPAEMYDVMKTCWDADPLKRPTFKQVVQLIEKQISDSTKHIYSNLANCNPNPENPVVVDHSVRVNSVGSSASSTQPLLVHEDA |
Keywords
- Cellular component
Phenotypes & Variants
Involvement in disease
Features
Showing features for natural variant, mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Natural variant | 207 | loss-of-function mutation abolishing ligand binding | ||||
Sequence: A → E | ||||||
Mutagenesis | 571 | Abolishes interaction with PTPN11/SHP-2. | ||||
Sequence: Y → F | ||||||
Mutagenesis | 573 | Abolishes interaction with PTPN6/SHP-1. | ||||
Sequence: Y → F | ||||||
Mutagenesis | 573 | Abolishes interaction with PTPN6/SHP-1. | ||||
Sequence: Missing | ||||||
Natural variant | 586 | in W37; impaired protein stability and loss of kinase activity | ||||
Sequence: E → K | ||||||
Natural variant | 664 | in Wv | ||||
Sequence: T → M | ||||||
Mutagenesis | 723 | Abolishes interaction with PIK3R1. | ||||
Sequence: Y → F | ||||||
Natural variant | 794 | in W42; loss of kinase activity and impaired internalization after exposure to KITLG/SCF | ||||
Sequence: D → N | ||||||
Natural variant | 835 | in W41; decreased kinase activity | ||||
Sequence: V → M | ||||||
Mutagenesis | 860 | Mice display white fur, hearing loss, anemia and mast cell deficiency, plus sterility in both males and females. | ||||
Sequence: F → S |
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 63 variants from UniProt as well as other sources including ClinVar and dbSNP.
Keywords
- Disease
Chemistry
PTM/Processing
Features
Showing features for signal, chain, disulfide bond, glycosylation, modified residue.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Signal | 1-24 | |||||
Sequence: MRGARGAWDLLCVLLVLLRGQTAT | ||||||
Chain | PRO_0000016755 | 25-979 | Mast/stem cell growth factor receptor Kit | |||
Sequence: SQPSASPGEPSPPSIHPAQSELIVEAGDTLSLTCIDPDFVRWTFKTYFNEMVENKKNEWIQEKAEATRTGTYTCSNSNGLTSSIYVFVRDPAKLFLVGLPLFGKEDSDALVRCPLTDPQVSNYSLIECDGKSLPTDLTFVPNPKAGITIKNVKRAYHRLCVRCAAQRDGTWLHSDKFTLKVRAAIKAIPVVSVPETSHLLKKGDTFTVVCTIKDVSTSVNSMWLKMNPQPQHIAQVKHNSWHRGDFNYERQETLTISSARVDDSGVFMCYANNTFGSANVTTTLKVVEKGFINISPVKNTTVFVTDGENVDLVVEYEAYPKPEHQQWIYMNRTSANKGKDYVKSDNKSNIRYVNQLRLTRLKGTEGGTYTFLVSNSDASASVTFNVYVNTKPEILTYDRLINGMLQCVAEGFPEPTIDWYFCTGAEQRCTTPVSPVDVQVQNVSVSPFGKLVVQSSIDSSVFRHNGTVECKASNDVGKSSAFFNFAFKGNNKEQIQAHTLFTPLLIGFVVAAGAMGIIVMVLTYKYLQKPMYEVQWKVVEEINGNNYVYIDPTQLPYDHKWEFPRNRLSFGKTLGAGAFGKVVEATAYGLIKSDAAMTVAVKMLKPSAHLTEREALMSELKVLSYLGNHMNIVNLLGACTVGGPTLVITEYCCYGDLLNFLRRKRDSFIFSKQEEQAEAALYKNLLHSTEPSCDSSNEYMDMKPGVSYVVPTKTDKRRSARIDSYIERDVTPAIMEDDELALDLDDLLSFSYQVAKGMAFLASKNCIHRDLAARNILLTHGRITKICDFGLARDIRNDSNYVVKGNARLPVKWMAPESIFSCVYTFESDVWSYGIFLWELFSLGSSPYPGMPVDSKFYKMIKEGFRMVSPEHAPAEMYDVMKTCWDADPLKRPTFKQVVQLIEKQISDSTKHIYSNLANCNPNPENPVVVDHSVRVNSVGSSASSTQPLLVHEDA | ||||||
Disulfide bond | 58↔98 | |||||
Sequence: CIDPDFVRWTFKTYFNEMVENKKNEWIQEKAEATRTGTYTC | ||||||
Disulfide bond | 137↔187 | |||||
Sequence: CPLTDPQVSNYSLIECDGKSLPTDLTFVPNPKAGITIKNVKRAYHRLCVRC | ||||||
Glycosylation | 146 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Disulfide bond | 152↔184 | |||||
Sequence: CDGKSLPTDLTFVPNPKAGITIKNVKRAYHRLC | ||||||
Disulfide bond | 234↔293 | |||||
Sequence: CTIKDVSTSVNSMWLKMNPQPQHIAQVKHNSWHRGDFNYERQETLTISSARVDDSGVFMC | ||||||
Glycosylation | 296 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Glycosylation | 303 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Glycosylation | 323 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Glycosylation | 355 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Glycosylation | 370 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Disulfide bond | 431↔494 | |||||
Sequence: CVAEGFPEPTIDWYFCTGAEQRCTTPVSPVDVQVQNVSVSPFGKLVVQSSIDSSVFRHNGTVEC | ||||||
Glycosylation | 466 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Glycosylation | 489 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Modified residue | 550 | Phosphotyrosine; by autocatalysis | ||||
Sequence: Y | ||||||
Modified residue | 556 | Phosphotyrosine; by autocatalysis | ||||
Sequence: Y | ||||||
Modified residue | 571 | Phosphotyrosine | ||||
Sequence: Y | ||||||
Modified residue | 573 | Phosphotyrosine | ||||
Sequence: Y | ||||||
Modified residue | 706 | Phosphotyrosine | ||||
Sequence: Y | ||||||
Modified residue | 720 | Phosphoserine | ||||
Sequence: S | ||||||
Modified residue | 723 | Phosphotyrosine; by autocatalysis | ||||
Sequence: Y | ||||||
Modified residue | 732 | Phosphotyrosine; by autocatalysis | ||||
Sequence: Y | ||||||
Modified residue | 743 | Phosphoserine; by PKC/PRKCA | ||||
Sequence: S | ||||||
Modified residue | 748 | Phosphoserine; by PKC/PRKCA | ||||
Sequence: S | ||||||
Modified residue | 823 | Phosphoserine | ||||
Sequence: S | ||||||
Modified residue | 825 | Phosphotyrosine; by autocatalysis | ||||
Sequence: Y | ||||||
Modified residue | 893 | Phosphoserine | ||||
Sequence: S | ||||||
Modified residue | 902 | Phosphotyrosine; by autocatalysis | ||||
Sequence: Y | ||||||
Modified residue | 938 | Phosphotyrosine | ||||
Sequence: Y | ||||||
Modified residue | 962 | Phosphoserine | ||||
Sequence: S |
Post-translational modification
Keywords
- PTM
Proteomic databases
PTM databases
Expression
Tissue specificity
Gene expression databases
Interaction
Subunit
Interacts with the protein kinase FES/FPS. Interacts with PLCG1. Interacts (via phosphorylated tyrosine residues) with PIK3R1 and PIK3 catalytic subunit. Interacts (KITLG/SCF-bound) with IL1RL1. Interacts with IL1RAP (independent of stimulation with KITLG/SCF). A mast cell-specific KITLG/SCF-induced interleukin-33 signaling complex contains IL1RL1, IL1RAP, KIT and MYD88
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | Intact | |
---|---|---|---|---|---|
BINARY | P05532 | Csf2rb P26955 | 4 | EBI-8559255, EBI-1810026 |
Protein-protein interaction databases
Chemistry
Miscellaneous
Structure
Family & Domains
Features
Showing features for domain, region.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 31-117 | Ig-like C2-type 1 | ||||
Sequence: PGEPSPPSIHPAQSELIVEAGDTLSLTCIDPDFVRWTFKTYFNEMVENKKNEWIQEKAEATRTGTYTCSNSNGLTSSIYVFVRDPAK | ||||||
Domain | 126-210 | Ig-like C2-type 2 | ||||
Sequence: FGKEDSDALVRCPLTDPQVSNYSLIECDGKSLPTDLTFVPNPKAGITIKNVKRAYHRLCVRCAAQRDGTWLHSDKFTLKVRAAIK | ||||||
Domain | 217-315 | Ig-like C2-type 3 | ||||
Sequence: VPETSHLLKKGDTFTVVCTIKDVSTSVNSMWLKMNPQPQHIAQVKHNSWHRGDFNYERQETLTISSARVDDSGVFMCYANNTFGSANVTTTLKVVEKGF | ||||||
Domain | 324-417 | Ig-like C2-type 4 | ||||
Sequence: TTVFVTDGENVDLVVEYEAYPKPEHQQWIYMNRTSANKGKDYVKSDNKSNIRYVNQLRLTRLKGTEGGTYTFLVSNSDASASVTFNVYVNTKPE | ||||||
Domain | 420-514 | Ig-like C2-type 5 | ||||
Sequence: TYDRLINGMLQCVAEGFPEPTIDWYFCTGAEQRCTTPVSPVDVQVQNVSVSPFGKLVVQSSIDSSVFRHNGTVECKASNDVGKSSAFFNFAFKGN | ||||||
Region | 571-573 | Important for interaction with phosphotyrosine-binding proteins | ||||
Sequence: YVY | ||||||
Domain | 592-939 | Protein kinase | ||||
Sequence: LSFGKTLGAGAFGKVVEATAYGLIKSDAAMTVAVKMLKPSAHLTEREALMSELKVLSYLGNHMNIVNLLGACTVGGPTLVITEYCCYGDLLNFLRRKRDSFIFSKQEEQAEAALYKNLLHSTEPSCDSSNEYMDMKPGVSYVVPTKTDKRRSARIDSYIERDVTPAIMEDDELALDLDDLLSFSYQVAKGMAFLASKNCIHRDLAARNILLTHGRITKICDFGLARDIRNDSNYVVKGNARLPVKWMAPESIFSCVYTFESDVWSYGIFLWELFSLGSSPYPGMPVDSKFYKMIKEGFRMVSPEHAPAEMYDVMKTCWDADPLKRPTFKQVVQLIEKQISDSTKHIYS |
Sequence similarities
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence & Isoforms
- Sequence statusComplete
- Sequence processingThe displayed sequence is further processed into a mature form.
This entry describes 3 isoforms produced by Alternative splicing.
P05532-1
This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
- Name1
- SynonymsGNNK(+), KitA(+)
- Length979
- Mass (Da)109,343
- Last updated2011-10-19 v3
- Checksum03FB4D672248585E
P05532-2
- Name2
- SynonymsGNNK(-), Kit(+)
- Differences from canonical
- 512-515: Missing
P05532-3
- Name3
- SynonymsTr-kit, Truncated
Features
Showing features for alternative sequence, sequence conflict.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Alternative sequence | VSP_041868 | 1-12 | in isoform 3 | |||
Sequence: MRGARGAWDLLC → MAVAVFPFLPQQ | ||||||
Alternative sequence | VSP_041869 | 13-789 | in isoform 3 | |||
Sequence: Missing | ||||||
Alternative sequence | VSP_041870 | 512-515 | in isoform 2 | |||
Sequence: Missing | ||||||
Sequence conflict | 551 | in Ref. 6; AAH52457 | ||||
Sequence: L → F | ||||||
Sequence conflict | 781 | in Ref. 1; CAA68772 and 2; CAA46799 | ||||
Sequence: G → A | ||||||
Sequence conflict | 860 | in Ref. 4; AAS45607 | ||||
Sequence: F → S |
Keywords
- Coding sequence diversity
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
Y00864 EMBL· GenBank· DDBJ | CAA68772.1 EMBL· GenBank· DDBJ | mRNA | ||
AK046795 EMBL· GenBank· DDBJ | BAC32872.1 EMBL· GenBank· DDBJ | mRNA | ||
X65997 EMBL· GenBank· DDBJ | CAA46798.1 EMBL· GenBank· DDBJ | mRNA | ||
X65998 EMBL· GenBank· DDBJ | CAA46799.1 EMBL· GenBank· DDBJ | Genomic DNA | Sequence problems. | |
X65998 EMBL· GenBank· DDBJ | CAA46800.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AY536430 EMBL· GenBank· DDBJ | AAS45606.1 EMBL· GenBank· DDBJ | mRNA | ||
AY536431 EMBL· GenBank· DDBJ | AAS45607.1 EMBL· GenBank· DDBJ | mRNA | ||
AC013622 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. | |
AC115853 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. | |
BC052457 EMBL· GenBank· DDBJ | AAH52457.1 EMBL· GenBank· DDBJ | mRNA | ||
BC075716 EMBL· GenBank· DDBJ | AAH75716.1 EMBL· GenBank· DDBJ | mRNA | ||
L11358 EMBL· GenBank· DDBJ | AAA37420.1 EMBL· GenBank· DDBJ | Genomic DNA |