P05195 · 3DHQ_NEUCR

Function

function

3-dehydroquinate dehydratase; part of the qa gene cluster that mediates the catabolism of quinic acid (QA) and as such, allows the use of QA as a sole carbon source (By similarity) (PubMed:126226, PubMed:143663, PubMed:2525625, PubMed:6458044).
Catalyzes the second reaction in the inducible quinic acid catabolic pathway by converting 3-dehydroquinate into 3-dehydroshikimate (PubMed:143663). The qa cluster encodes 3 inducible enymes (qa-2, qa-3 and qa-4) catalyzing the first three reactions in the catabolism of quinic acid to protocatechuic acid (also known as 3,4-Dihydroxybenzoic acid) (Probable)

Catalytic activity

Kinetics

KM SUBSTRATE pH TEMPERATURE[C] NOTES EVIDENCE
450 μMquinate
1.7 mMshikimate
150 μMNAD+

Pathway

Aromatic compound metabolism; 3,4-dihydroxybenzoate biosynthesis; 3,4-dihydroxybenzoate from 3-dehydroquinate: step 1/2.

Features

Showing features for site, active site, binding site.

TypeIDPosition(s)Description
Site21Transition state stabilizer
Active site26Proton acceptor
Binding site102substrate
Binding site108substrate
Binding site115substrate
Active site128Proton donor
Binding site129-130substrate
Binding site139substrate

GO annotations

AspectTerm
Molecular Function3-dehydroquinate dehydratase activity
Biological Process3,4-dihydroxybenzoate biosynthetic process
Biological Processquinate catabolic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Catabolic 3-dehydroquinase
  • EC number
  • Short names
    cDHQase
  • Alternative names
    • 3-dehydroquinate dehydratase
    • Quinic acid degradation cluster protein 2f

Gene names

    • Name
      qa-2
    • ORF names
      NCU06023

Organism names

Accessions

  • Primary accession
    P05195
  • Secondary accessions
    • Q7RVA3

Proteomes

    • Identifier
    • Component
      Chromosome 7, Linkage Group VII

Organism-specific databases

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00001599481-173Catabolic 3-dehydroquinase

Proteomic databases

Expression

Induction

Expression is induced in the presence of quinic acid (PubMed:144915, PubMed:6458044).
The quinic acid (qa) gene cluster is subject to two levels of gene control: a primary system which responds to the presence of quinic acid via the qa-1S repressor protein that blocks the qa-1F activator, and a secondary system which represses transcription of qa genes in the presence of a preferred carbon source such as glucose (PubMed:12477937, PubMed:17597928, PubMed:19236936, PubMed:6458044).

Interaction

Subunit

Homododecamer. Adopts a ring-like structure, composed of an arrangement of two hexameric rings stacked on top of one another.

Protein-protein interaction databases

Structure

Family & Domains

Sequence similarities

Belongs to the type-II 3-dehydroquinase family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    173
  • Mass (Da)
    18,272
  • Last updated
    1989-10-01 v2
  • Checksum
    6A6EC7594DD46F1B
MASPRHILLINGPNLNLLGTREPQIYGSTTLHDIEQASQTLASSLGLRLTTFQSNHEGAIIDRIHQAAGFVPSPPSPSPSSAATTTEAGLGPGDKVSAIIINPGAYTHTSIGIRDALLGTGIPFVEVHVSNVHAREAFRHHSYLSDKAVAVICGLGPFGYSAALDFLGRHMKF

Features

Showing features for sequence conflict.

TypeIDPosition(s)Description
Sequence conflict25-29in Ref. 3; CAA24237

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
X14603
EMBL· GenBank· DDBJ
CAA32749.1
EMBL· GenBank· DDBJ
Genomic DNA
V00869
EMBL· GenBank· DDBJ
CAA24237.1
EMBL· GenBank· DDBJ
Genomic DNA
CM002242
EMBL· GenBank· DDBJ
EAA30377.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

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