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P04631 · S100B_RAT

Function

function

Small zinc- and- and calcium-binding protein that is highly expressed in astrocytes and constitutes one of the most abundant soluble proteins in brain (PubMed:14621986, PubMed:15823027, PubMed:18949447, PubMed:20351179).
Weakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer (PubMed:15823027).
Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites (By similarity).
Acts as a neurotrophic factor that promotes astrocytosis and axonal proliferation (By similarity).
Involved in innervation of thermogenic adipose tissue by acting as an adipocyte-derived neurotrophic factor that promotes sympathetic innervation of adipose tissue (By similarity).
Binds to and initiates the activation of STK38 by releasing autoinhibitory intramolecular interactions within the kinase (By similarity).
Interaction with AGER after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling (PubMed:19910580).
Could assist ATAD3A cytoplasmic processing, preventing aggregation and favoring mitochondrial localization (By similarity).
May mediate calcium-dependent regulation on many physiological processes by interacting with other proteins, such as TPR-containing proteins, and modulating their activity (By similarity).

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site16Zn2+ (UniProtKB | ChEBI)
Binding site19Ca2+ 1 (UniProtKB | ChEBI); low affinity
Binding site22Ca2+ 1 (UniProtKB | ChEBI); low affinity
Binding site26Zn2+ (UniProtKB | ChEBI)
Binding site27Ca2+ 1 (UniProtKB | ChEBI); low affinity
Binding site32Ca2+ 1 (UniProtKB | ChEBI); low affinity
Binding site62Ca2+ 2 (UniProtKB | ChEBI); high affinity
Binding site64Ca2+ 2 (UniProtKB | ChEBI); high affinity
Binding site66Ca2+ 2 (UniProtKB | ChEBI); high affinity
Binding site68Ca2+ 2 (UniProtKB | ChEBI); high affinity
Binding site73Ca2+ 2 (UniProtKB | ChEBI); high affinity
Binding site86Zn2+ (UniProtKB | ChEBI)
Binding site91Zn2+ (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytoplasm
Cellular Componentextracellular region
Cellular Componentextracellular space
Cellular Componentneuronal cell body
Cellular Componentnucleus
Cellular Componentperinuclear region of cytoplasm
Cellular Componentruffle
Molecular Functioncalcium ion binding
Molecular Functioncalcium-dependent protein binding
Molecular Functionidentical protein binding
Molecular Functionprotein homodimerization activity
Molecular FunctionRAGE receptor binding
Molecular FunctionS100 protein binding
Molecular Functionsignaling receptor binding
Molecular Functiontau protein binding
Molecular Functionzinc ion binding
Biological Processadaptive thermogenesis
Biological Processastrocyte differentiation
Biological Processcell adhesion
Biological Processcellular response to hypoxia
Biological Processlearning or memory
Biological Processlong-term synaptic potentiation
Biological Processmemory
Biological Processnegative regulation of skeletal muscle cell differentiation
Biological Processneuron projection extension
Biological Processpositive regulation of apoptotic process
Biological Processpositive regulation of canonical NF-kappaB signal transduction
Biological Processpositive regulation of cell population proliferation
Biological Processpositive regulation of myelination
Biological Processpositive regulation of neuron differentiation
Biological Processpositive regulation of synaptic transmission
Biological Processregulation of cell shape
Biological Processregulation of neuronal synaptic plasticity
Biological Processresponse to glucocorticoid
Biological Processresponse to methylmercury
Biological Processsympathetic neuron projection extension

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Protein S100-B
  • Alternative names
    • S-100 protein beta chain
    • S-100 protein subunit beta
    • S100 calcium-binding protein B

Gene names

    • Name
      S100b

Organism names

  • Taxonomic identifier
  • Strain
    • Sprague-Dawley
  • Taxonomic lineage
    Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Rattus

Accessions

  • Primary accession
    P04631

Proteomes

Organism-specific databases

Subcellular Location

Cytoplasm
Nucleus
Secreted
Note: Secretion into the medium is promoted by interaction with isoform CLSTN3beta of CLSTN3.

Keywords

Phenotypes & Variants

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis16Decreased zinc-binding.
Mutagenesis26Decreased zinc-binding.
Mutagenesis43Does not affect zinc-binding.
Mutagenesis69Does not affect zinc-binding.
Mutagenesis85Decreased zinc-binding.
Mutagenesis86Decreased zinc-binding.
Mutagenesis91Decreased zinc-binding.

Chemistry

PTM/Processing

Features

Showing features for initiator methionine, modified residue, chain.

Type
IDPosition(s)Description
Initiator methionine1Removed
Modified residue2N-acetylserine
ChainPRO_00001439692-92Protein S100-B

Keywords

Proteomic databases

PTM databases

Expression

Tissue specificity

Although predominant among the water-soluble brain proteins, S100 is also found in a variety of other tissues.

Induction

Up-regulated in periinfarct ventricular myocardium.

Gene expression databases

Interaction

Subunit

Dimer of either two alpha chains, or two beta chains, or one alpha and one beta chain (By similarity).
The S100B dimer binds two molecules of STK38 (By similarity).
Interacts with CACYBP in a calcium-dependent manner (PubMed:12042313).
Interacts with ATAD3A; this interaction probably occurs in the cytosol prior to ATAD3A mitochondrial targeting (PubMed:20351179).
Interacts with S100A6 (By similarity).
The S100B dimer interacts with two molecules of CAPZA1 (PubMed:12470955).
Interacts with AGER (PubMed:19910580).
Interacts with PPP5C (via TPR repeats); the interaction is calcium-dependent and modulates PPP5C activity (By similarity).
Interacts with TPPP; this interaction inhibits TPPP dimerization (By similarity).
Interacts with isoform CLSTN3beta of CLSTN3; interaction promotes secretion (By similarity).

Binary interactions

TypeEntry 1Entry 2Number of experimentsIntAct
BINARY P04631Ager Q634953EBI-2696631, EBI-6479195

Protein-protein interaction databases

Chemistry

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain13-48EF-hand 1
Domain49-84EF-hand 2

Sequence similarities

Belongs to the S-100 family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    92
  • Mass (Da)
    10,744
  • Last updated
    2007-01-23 v2
  • MD5 Checksum
    D2C1CA37FDDD73CA81E7C01EE30D76EC
MSELEKAMVALIDVFHQYSGREGDKHKLKKSELKELINNELSHFLEEIKEQEVVDKVMETLDEDGDGECDFQEFMAFVSMVTTACHEFFEHE

Computationally mapped potential isoform sequences

There are 2 potential isoforms mapped to this entry

View all
EntryEntry nameGene nameLength
A0A8I6A0X7A0A8I6A0X7_RATS100b185
A0A8L2PZQ2A0A8L2PZQ2_RATS100b187

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
X01090
EMBL· GenBank· DDBJ
CAA25567.1
EMBL· GenBank· DDBJ
mRNA
M54919
EMBL· GenBank· DDBJ
AAA42096.1
EMBL· GenBank· DDBJ
mRNA
S53527
EMBL· GenBank· DDBJ
-Genomic DNA No translation available.
S53522
EMBL· GenBank· DDBJ
-Genomic DNA No translation available.
BC087026
EMBL· GenBank· DDBJ
AAH87026.1
EMBL· GenBank· DDBJ
mRNA
M15705
EMBL· GenBank· DDBJ
-mRNA No translation available.

Genome annotation databases

Similar Proteins

Disclaimer

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