P04631 · S100B_RAT
- ProteinProtein S100-B
- GeneS100b
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Small zinc- and- and calcium-binding protein that is highly expressed in astrocytes and constitutes one of the most abundant soluble proteins in brain (PubMed:14621986, PubMed:15823027, PubMed:18949447, PubMed:20351179).
Weakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer (PubMed:15823027).
Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites (By similarity).
Acts as a neurotrophic factor that promotes astrocytosis and axonal proliferation (By similarity).
Involved in innervation of thermogenic adipose tissue by acting as an adipocyte-derived neurotrophic factor that promotes sympathetic innervation of adipose tissue (By similarity).
Binds to and initiates the activation of STK38 by releasing autoinhibitory intramolecular interactions within the kinase (By similarity).
Interaction with AGER after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling (PubMed:19910580).
Could assist ATAD3A cytoplasmic processing, preventing aggregation and favoring mitochondrial localization (By similarity).
May mediate calcium-dependent regulation on many physiological processes by interacting with other proteins, such as TPR-containing proteins, and modulating their activity (By similarity).
Weakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer (PubMed:15823027).
Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites (By similarity).
Acts as a neurotrophic factor that promotes astrocytosis and axonal proliferation (By similarity).
Involved in innervation of thermogenic adipose tissue by acting as an adipocyte-derived neurotrophic factor that promotes sympathetic innervation of adipose tissue (By similarity).
Binds to and initiates the activation of STK38 by releasing autoinhibitory intramolecular interactions within the kinase (By similarity).
Interaction with AGER after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling (PubMed:19910580).
Could assist ATAD3A cytoplasmic processing, preventing aggregation and favoring mitochondrial localization (By similarity).
May mediate calcium-dependent regulation on many physiological processes by interacting with other proteins, such as TPR-containing proteins, and modulating their activity (By similarity).
Features
Showing features for binding site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 16 | Zn2+ (UniProtKB | ChEBI) | |||
Binding site | 19 | Ca2+ 1 (UniProtKB | ChEBI); low affinity | |||
Binding site | 22 | Ca2+ 1 (UniProtKB | ChEBI); low affinity | |||
Binding site | 26 | Zn2+ (UniProtKB | ChEBI) | |||
Binding site | 27 | Ca2+ 1 (UniProtKB | ChEBI); low affinity | |||
Binding site | 32 | Ca2+ 1 (UniProtKB | ChEBI); low affinity | |||
Binding site | 62 | Ca2+ 2 (UniProtKB | ChEBI); high affinity | |||
Binding site | 64 | Ca2+ 2 (UniProtKB | ChEBI); high affinity | |||
Binding site | 66 | Ca2+ 2 (UniProtKB | ChEBI); high affinity | |||
Binding site | 68 | Ca2+ 2 (UniProtKB | ChEBI); high affinity | |||
Binding site | 73 | Ca2+ 2 (UniProtKB | ChEBI); high affinity | |||
Binding site | 86 | Zn2+ (UniProtKB | ChEBI) | |||
Binding site | 91 | Zn2+ (UniProtKB | ChEBI) | |||
GO annotations
Keywords
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameProtein S100-B
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Rattus
Accessions
- Primary accessionP04631
Proteomes
Organism-specific databases
Phenotypes & Variants
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Mutagenesis | 16 | Decreased zinc-binding. | |||
Mutagenesis | 26 | Decreased zinc-binding. | |||
Mutagenesis | 43 | Does not affect zinc-binding. | |||
Mutagenesis | 69 | Does not affect zinc-binding. | |||
Mutagenesis | 85 | Decreased zinc-binding. | |||
Mutagenesis | 86 | Decreased zinc-binding. | |||
Mutagenesis | 91 | Decreased zinc-binding. | |||
Chemistry
PTM/Processing
Features
Showing features for initiator methionine, modified residue, chain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Initiator methionine | 1 | Removed | |||
Modified residue | 2 | N-acetylserine | |||
Chain | PRO_0000143969 | 2-92 | Protein S100-B | ||
Keywords
- PTM
Proteomic databases
PTM databases
Expression
Tissue specificity
Although predominant among the water-soluble brain proteins, S100 is also found in a variety of other tissues.
Induction
Up-regulated in periinfarct ventricular myocardium.
Gene expression databases
Interaction
Subunit
Dimer of either two alpha chains, or two beta chains, or one alpha and one beta chain (By similarity).
The S100B dimer binds two molecules of STK38 (By similarity).
Interacts with CACYBP in a calcium-dependent manner (PubMed:12042313).
Interacts with ATAD3A; this interaction probably occurs in the cytosol prior to ATAD3A mitochondrial targeting (PubMed:20351179).
Interacts with S100A6 (By similarity).
The S100B dimer interacts with two molecules of CAPZA1 (PubMed:12470955).
Interacts with AGER (PubMed:19910580).
Interacts with PPP5C (via TPR repeats); the interaction is calcium-dependent and modulates PPP5C activity (By similarity).
Interacts with TPPP; this interaction inhibits TPPP dimerization (By similarity).
Interacts with isoform CLSTN3beta of CLSTN3; interaction promotes secretion (By similarity).
The S100B dimer binds two molecules of STK38 (By similarity).
Interacts with CACYBP in a calcium-dependent manner (PubMed:12042313).
Interacts with ATAD3A; this interaction probably occurs in the cytosol prior to ATAD3A mitochondrial targeting (PubMed:20351179).
Interacts with S100A6 (By similarity).
The S100B dimer interacts with two molecules of CAPZA1 (PubMed:12470955).
Interacts with AGER (PubMed:19910580).
Interacts with PPP5C (via TPR repeats); the interaction is calcium-dependent and modulates PPP5C activity (By similarity).
Interacts with TPPP; this interaction inhibits TPPP dimerization (By similarity).
Interacts with isoform CLSTN3beta of CLSTN3; interaction promotes secretion (By similarity).
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | IntAct | |
---|---|---|---|---|---|
BINARY | P04631 | Ager Q63495 | 3 | EBI-2696631, EBI-6479195 |
Protein-protein interaction databases
Chemistry
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Domain | 13-48 | EF-hand 1 | |||
Domain | 49-84 | EF-hand 2 | |||
Sequence similarities
Belongs to the S-100 family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length92
- Mass (Da)10,744
- Last updated2007-01-23 v2
- MD5 ChecksumD2C1CA37FDDD73CA81E7C01EE30D76EC
Computationally mapped potential isoform sequences
There are 2 potential isoforms mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
A0A8I6A0X7 | A0A8I6A0X7_RAT | S100b | 185 | ||
A0A8L2PZQ2 | A0A8L2PZQ2_RAT | S100b | 187 |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
X01090 EMBL· GenBank· DDBJ | CAA25567.1 EMBL· GenBank· DDBJ | mRNA | ||
M54919 EMBL· GenBank· DDBJ | AAA42096.1 EMBL· GenBank· DDBJ | mRNA | ||
S53527 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. | |
S53522 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. | |
BC087026 EMBL· GenBank· DDBJ | AAH87026.1 EMBL· GenBank· DDBJ | mRNA | ||
M15705 EMBL· GenBank· DDBJ | - | mRNA | No translation available. |