P04586 · POL_HV1Z6
- ProteinGag-Pol polyprotein
- Genegag-pol
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids148 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score3/5
Function
function
Integrase
Catalyzes viral DNA integration into the host chromosome, by performing a series of DNA cutting and joining reactions. This enzyme activity takes place after virion entry into a cell and reverse transcription of the RNA genome in dsDNA. The first step in the integration process is 3' processing. This step requires a complex comprising the viral genome, matrix protein, Vpr and integrase. This complex is called the pre-integration complex (PIC). The integrase protein removes 2 nucleotides from each 3' end of the viral DNA, leaving recessed CA OH's at the 3' ends. In the second step, the PIC enters cell nucleus. This process is mediated through integrase and Vpr proteins, and allows the virus to infect a non dividing cell. This ability to enter the nucleus is specific of lentiviruses, other retroviruses cannot and rely on cell division to access cell chromosomes. In the third step, termed strand transfer, the integrase protein joins the previously processed 3' ends to the 5' ends of strands of target cellular DNA at the site of integration. The 5'-ends are produced by integrase-catalyzed staggered cuts, 5 bp apart. A Y-shaped, gapped, recombination intermediate results, with the 5'-ends of the viral DNA strands and the 3' ends of target DNA strands remaining unjoined, flanking a gap of 5 bp. The last step is viral DNA integration into host chromosome. This involves host DNA repair synthesis in which the 5 bp gaps between the unjoined strands are filled in and then ligated. Since this process occurs at both cuts flanking the HIV genome, a 5 bp duplication of host DNA is produced at the ends of HIV-1 integration. Alternatively, Integrase may catalyze the excision of viral DNA just after strand transfer, this is termed disintegration.
Features
Showing features for binding site, dna binding.
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | DNA binding | |
Molecular Function | endonuclease activity | |
Molecular Function | lipid binding | |
Molecular Function | metal ion binding | |
Molecular Function | nucleotidyltransferase activity | |
Biological Process | DNA integration | |
Biological Process | DNA recombination | |
Biological Process | establishment of integrated proviral latency | |
Biological Process | symbiont entry into host cell | |
Biological Process | symbiont-mediated suppression of host gene expression | |
Biological Process | viral genome integration into host DNA |
Keywords
- Molecular function
- Biological process
- Ligand
Protein family/group databases
Names & Taxonomy
Protein names
- Recommended nameGag-Pol polyprotein
- Alternative names
- Cleaved into 1 chains
Gene names
Organism names
- Taxonomic lineageViruses > Riboviria > Pararnavirae > Artverviricota > Revtraviricetes > Ortervirales > Retroviridae > Orthoretrovirinae > Lentivirus > Human immunodeficiency virus 1
- Virus hosts
Accessions
- Primary accessionP04586
Phenotypes & Variants
Keywords
- Disease
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000261289 | 1-148 | Gag-Pol polyprotein | |||
Sequence: IPYNPQSQGVVESMNKELKKIIGQVRDQAEHLKTAVQMAVFIHNFKRKGGIGGYSAGERIIDIIATDIQTKELQKQITKIQNFRVYYRDSRDPIWKGPAKLLWKGEGAVVIQDNSDIKVVPRRKVKIIRDYGKQMAGDDCVASRQDED | ||||||
Chain | PRO_0000042448 | 1-148 | Integrase | |||
Sequence: IPYNPQSQGVVESMNKELKKIIGQVRDQAEHLKTAVQMAVFIHNFKRKGGIGGYSAGERIIDIIATDIQTKELQKQITKIQNFRVYYRDSRDPIWKGPAKLLWKGEGAVVIQDNSDIKVVPRRKVKIIRDYGKQMAGDDCVASRQDED |
Post-translational modification
Specific enzymatic cleavages by the viral protease yield mature proteins.
Interaction
Subunit
Integrase
Homotetramer; may further associate as a homohexadecamer (By similarity).
Part of the pre-integration complex (PIC) which is composed of viral genome, matrix protein, Vpr and integrase. Interacts with human SMARCB1/INI1 and human PSIP1/LEDGF isoform 1. Interacts with human KPNA3; this interaction might play a role in nuclear import of the pre-integration complex (By similarity).
Interacts with human NUP153; this interaction might play a role in nuclear import of the pre-integration complex (By similarity).
Part of the pre-integration complex (PIC) which is composed of viral genome, matrix protein, Vpr and integrase. Interacts with human SMARCB1/INI1 and human PSIP1/LEDGF isoform 1. Interacts with human KPNA3; this interaction might play a role in nuclear import of the pre-integration complex (By similarity).
Interacts with human NUP153; this interaction might play a role in nuclear import of the pre-integration complex (By similarity).
Structure
Sequence
- Sequence statusFragment
- Length148
- Mass (Da)16,840
- Last updated1987-08-13 v1
- ChecksumA0DD31618CF0FC0E
Features
Showing features for non-terminal residue.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Non-terminal residue | 1 | |||||
Sequence: I |
Keywords
- Technical term