P04392 · DMA_BPT4

Function

function

An alpha subtype methylase, recognizes the double-stranded sequence 5'-GATC-3' and methylates A-2. Also acts on 5-hydroxymethylcytosine (hmC)-containing DNA, the normal base in this virus (PubMed:12654995, PubMed:2510127).
May prevent degradation of viral DNA by the host restriction-modification antiviral defense system (Probable)

Catalytic activity

Kinetics

KM SUBSTRATE pH TEMPERATURE[C] NOTES EVIDENCE
0.1 μMS-adenosylmethionine

pH Dependence

Optimum pH is 7.0-8.5.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site7S-adenosyl-L-methionine (UniProtKB | ChEBI)
Binding site11S-adenosyl-L-methionine (UniProtKB | ChEBI)
Binding site32-37S-adenosyl-L-methionine (UniProtKB | ChEBI)
Binding site50S-adenosyl-L-methionine (UniProtKB | ChEBI)
Binding site156-157S-adenosyl-L-methionine (UniProtKB | ChEBI)
Binding site171S-adenosyl-L-methionine (UniProtKB | ChEBI)
Binding site181S-adenosyl-L-methionine (UniProtKB | ChEBI)

GO annotations

AspectTerm
Molecular FunctionDNA binding
Molecular FunctionDNA-methyltransferase activity
Molecular Functionsite-specific DNA-methyltransferase (adenine-specific) activity
Biological ProcessDNA replication
Biological ProcessDNA restriction-modification system
Biological Processmethylation
Biological Processsymbiont-mediated evasion of host restriction-modification system
Biological Processsymbiont-mediated suppression of host innate immune response
Biological Processvirus-mediated perturbation of host defense response

Keywords

Enzyme and pathway databases

Protein family/group databases

Names & Taxonomy

Protein names

  • Recommended name
    DNA adenine methylase
  • EC number
  • Alternative names
    • DNA-(N(6)-adenine)-methyltransferase
    • Deoxyadenosyl-methyltransferase
    • Orphan methyltransferase M.EcoT4Dam
      (M.EcoT4Dam
      )

Gene names

    • Name
      DAM

Organism names

Accessions

  • Primary accession
    P04392

Proteomes

Phenotypes & Variants

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis126Hypermethylates DNA.
Mutagenesis126Loss of methylase activity.
Mutagenesis126In damh; hypermethylating mutant.
Mutagenesis127No longer methylates hmC-DNA-containing DNA.

Chemistry

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00000879901-259DNA adenine methylase

Interaction

Subunit

Monomer.

Structure

Family & Domains

Sequence similarities

Belongs to the N(4)/N(6)-methyltransferase family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    259
  • Mass (Da)
    30,417
  • Last updated
    1987-03-20 v1
  • Checksum
    A49401D059A4388D
MLGAIAYTGNKQSLLPELKSHFPKYNRFVDLFCGGLSVSLNVNGPVLANDIQEPIIEMYKRLINVSWDDVLKVIKQYKLSKTSKEEFLKLREDYNKTRDPLLLYVLHFHGFSNMIRINDKGNFTTPFGKRTINKNSEKQYNHFKQNCDKIIFSSLHFKDVKILDGDFVYVDPPYLITVADYNKFWSEDEEKDLLNLLDSLNDRGIKFGQSNVLEHHGKENTLLKEWSKKYNVKHLNKKYVFNIYHSKEKNGTDEVYIFN

Features

Showing features for sequence conflict.

TypeIDPosition(s)Description
Sequence conflict139-140in Ref. 2; X17641
Sequence conflict209in Ref. 2; X17641

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
X01416
EMBL· GenBank· DDBJ
CAA25660.1
EMBL· GenBank· DDBJ
Genomic DNA
X17641
EMBL· GenBank· DDBJ
-Genomic DNA No translation available.
K03113
EMBL· GenBank· DDBJ
AAA32555.1
EMBL· GenBank· DDBJ
Genomic DNA
AF158101
EMBL· GenBank· DDBJ
AAD42553.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

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