P04363 · KITH_MONPZ
- ProteinThymidine kinase
- GeneOPG101
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids177 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Phosphorylates thymidine and thymidine analogs, such as azidothymidine (AZT). Part of the salvage pathway for pyrimidine deoxyribonucleotide synthesis.
Catalytic activity
- ATP + thymidine = ADP + dTMP + H+
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 11-18 | ATP (UniProtKB | ChEBI) | ||||
Sequence: GPMLSGKS | ||||||
Active site | 83 | Proton acceptor | ||||
Sequence: E | ||||||
Binding site | 113 | substrate | ||||
Sequence: F | ||||||
Binding site | 138 | Zn2+ (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 141 | Zn2+ (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 157-161 | substrate | ||||
Sequence: IEIIG | ||||||
Binding site | 170 | Zn2+ (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 173 | Zn2+ (UniProtKB | ChEBI) | ||||
Sequence: C |
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Molecular Function | ATP binding | |
Molecular Function | metal ion binding | |
Molecular Function | thymidine kinase activity | |
Biological Process | DNA biosynthetic process | |
Biological Process | thymidine metabolic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameThymidine kinase
- EC number
Gene names
Organism names
- Strain
- Taxonomic lineageViruses > Varidnaviria > Bamfordvirae > Nucleocytoviricota > Pokkesviricetes > Chitovirales > Poxviridae > Chordopoxvirinae > Orthopoxvirus > Monkeypox virus
- Virus hosts
Accessions
- Primary accessionP04363
- Secondary accessions
Proteomes
PTM/Processing
Features
Showing features for chain, disulfide bond.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000174931 | 1-177 | Thymidine kinase | |||
Sequence: MNGGHIQLIIGPMLSGKSTELIRRVRRYQIAQYKCVTIKYSNDNRYGTGLWTHDKNNFAALEVTKLCDVLEAITDFSVIGIDEGQFFPDIVEFCERMANEGKIVIVAALDGTFQRRPFNNILNLIPLSEMVVKLTAVCMKCFKEASFSKRLGAETEIEIIGGNDMYQSVCRKCYIDS | ||||||
Disulfide bond | 170 | Interchain (with C-173) | ||||
Sequence: C | ||||||
Disulfide bond | 173 | Interchain (with C-170) | ||||
Sequence: C |
Keywords
- PTM
Interaction
Subunit
Homotetramer. Two molecules of substrate bind to each enzyme tetramer.
Sequence
- Sequence statusComplete
- Length177
- Mass (Da)19,990
- Last updated2002-08-02 v2
- Checksum8A7BAAB3238C91D2
Features
Showing features for sequence conflict.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Sequence conflict | 14 | in Ref. 1; AAA80529 | ||||
Sequence: L → F | ||||||
Sequence conflict | 90 | in Ref. 1; AAA80529 | ||||
Sequence: I → V | ||||||
Sequence conflict | 153 | in Ref. 1; AAA80529 | ||||
Sequence: A → T |
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
K02025 EMBL· GenBank· DDBJ | AAA80529.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AF380138 EMBL· GenBank· DDBJ | AAL40544.1 EMBL· GenBank· DDBJ | Genomic DNA |