P03872 · REP2_YEAST
- ProteinPartitioning protein REP2
- GeneREP2
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids296 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
Part of the plasmid partitioning system, which ensures the equal distribution of replicated plasmid molecules to daughter cells. The plasmids exist as well-organized plasmid foci within the nucleus that stay together throughout the cell-cycle and act as entity during segregation, effetively reducing copy number to one. Plasmid partitioning requires the proteins REP1, REP2, and a cis-acting locus STB (REP3). REP1-REP2 stably associate with CSE4-containing chromatin at STB during S-phase, marking the locus with a centromeric tag, and thereby probably catching mitotic spindle microtubules to the plasmid cluster and coupling plasmid segregation to chromosome segregation. REP1-REP2 are required to recruit the cohesin complex to the STB locus for pairing of the replicated plasmid cluster, a prerequisite for successful plasmid segregation. REP1-REP2 also negatively regulate expression of site-specific recombinase FLP and of RAF1.
Miscellaneous
The plasmid 2-micron circle is a extrachromosomal element that resides in the nucleus and propagates itself stably in host cell populations. It provides no obvious advantage to the host but imposes no significant disadvantage either at its steady-state copy number of 40-60 molecules/cell.
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | nucleus | |
Molecular Function | identical protein binding | |
Biological Process | 2-micrometer plasmid partitioning | |
Biological Process | protein stabilization |
Keywords
- Biological process
Names & Taxonomy
Protein names
- Recommended namePartitioning protein REP2
- Short namesR2
- Alternative names
Gene names
Encoded on
- Plasmid 2-micron
Organism names
- Strain
- Taxonomic lineageEukaryota > Fungi > Dikarya > Ascomycota > Saccharomycotina > Saccharomycetes > Saccharomycetales > Saccharomycetaceae > Saccharomyces
Accessions
- Primary accessionP03872
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Note: Colocalizes with the STB locus of the 2-micron plasmid as foci in the nucleus near the spindle pole body. Is expelled from STB during a short interval between late G1 and early S phases.
Keywords
- Cellular component
PTM/Processing
Features
Showing features for modified residue, chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Modified residue | 1 | N-acetylmethionine | ||||
Sequence: M | ||||||
Chain | PRO_0000150898 | 1-296 | Partitioning protein REP2 | |||
Sequence: MDDIETAKNLTVKARTAYSVWDVCRLFIEMIAPDVDIDIESKRKSDELLFPGYVIRPMESLTTGRPYGLDSSAEDSSVSSDSSAEVILPAAKMVKERFDSIGNGMLSSQEASQAAIDLMLQNNKLLDNRKQLYKSIAIIIGRLPEKDKKRATEMLMRKMDCTQLLVPPAPTEEDVMKLVSVVTQLLTLVPPDRQAALIGDLFIPESLKDIFNSFNELAAENRLQQKKSELEGRTEVNHANTNEEVPSRRTRSRDTNARGAYKLQNTITEGPKAVPTKKRRVATRVRGRKSRNTSRV |
Keywords
- PTM
Proteomic databases
PTM databases
Interaction
Subunit
Interacts with REP1.
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | Intact | |
---|---|---|---|---|---|
BINARY | P03872 | REP1 P03871 | 7 | EBI-2125362, EBI-14929 | |
BINARY | P03872 | REP2 P03872 | 5 | EBI-2125362, EBI-2125362 |
Protein-protein interaction databases
Miscellaneous
Structure
Family & Domains
Features
Showing features for region, compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 1-57 | Interaction with REP1 | ||||
Sequence: MDDIETAKNLTVKARTAYSVWDVCRLFIEMIAPDVDIDIESKRKSDELLFPGYVIRP | ||||||
Region | 58-296 | DNA-binding, and self-association | ||||
Sequence: MESLTTGRPYGLDSSAEDSSVSSDSSAEVILPAAKMVKERFDSIGNGMLSSQEASQAAIDLMLQNNKLLDNRKQLYKSIAIIIGRLPEKDKKRATEMLMRKMDCTQLLVPPAPTEEDVMKLVSVVTQLLTLVPPDRQAALIGDLFIPESLKDIFNSFNELAAENRLQQKKSELEGRTEVNHANTNEEVPSRRTRSRDTNARGAYKLQNTITEGPKAVPTKKRRVATRVRGRKSRNTSRV | ||||||
Compositional bias | 228-256 | Basic and acidic residues | ||||
Sequence: SELEGRTEVNHANTNEEVPSRRTRSRDTN | ||||||
Region | 228-296 | Disordered | ||||
Sequence: SELEGRTEVNHANTNEEVPSRRTRSRDTNARGAYKLQNTITEGPKAVPTKKRRVATRVRGRKSRNTSRV | ||||||
Region | 276-296 | Nuclear localization | ||||
Sequence: TKKRRVATRVRGRKSRNTSRV | ||||||
Compositional bias | 277-296 | Basic residues | ||||
Sequence: KKRRVATRVRGRKSRNTSRV |
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length296
- Mass (Da)33,196
- Last updated1986-07-21 v1
- ChecksumFF54CBC6B90F5FAF
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 228-256 | Basic and acidic residues | ||||
Sequence: SELEGRTEVNHANTNEEVPSRRTRSRDTN | ||||||
Compositional bias | 277-296 | Basic residues | ||||
Sequence: KKRRVATRVRGRKSRNTSRV |
Keywords
- Technical term