P03872 · REP2_YEAST

Function

function

Part of the plasmid partitioning system, which ensures the equal distribution of replicated plasmid molecules to daughter cells. The plasmids exist as well-organized plasmid foci within the nucleus that stay together throughout the cell-cycle and act as entity during segregation, effetively reducing copy number to one. Plasmid partitioning requires the proteins REP1, REP2, and a cis-acting locus STB (REP3). REP1-REP2 stably associate with CSE4-containing chromatin at STB during S-phase, marking the locus with a centromeric tag, and thereby probably catching mitotic spindle microtubules to the plasmid cluster and coupling plasmid segregation to chromosome segregation. REP1-REP2 are required to recruit the cohesin complex to the STB locus for pairing of the replicated plasmid cluster, a prerequisite for successful plasmid segregation. REP1-REP2 also negatively regulate expression of site-specific recombinase FLP and of RAF1.

Miscellaneous

The plasmid 2-micron circle is a extrachromosomal element that resides in the nucleus and propagates itself stably in host cell populations. It provides no obvious advantage to the host but imposes no significant disadvantage either at its steady-state copy number of 40-60 molecules/cell.

GO annotations

all annotationsall molecular functionvirus receptor activitydna bindingrna bindingcytoskeletal motor activitycatalytic activitygtpase activitystructural molecule activitytransporter activitycytoskeletal protein bindinglipid bindingcyclase activityantioxidant activityoxidoreductase activitytransferase activityhydrolase activitylyase activityisomerase activityligase activityprotein tag activitycargo receptor activityhistone bindingprotein folding chaperonetranslation regulator activitynutrient reservoir activityreceptor ligand activitymolecular transducer activitymolecular adaptor activitytoxin activitycell adhesion mediator activitymolecular function regulator activityvirus coreceptor activitycatalytic activity, acting on a proteincatalytic activity, acting on dnacatalytic activity, acting on rnamolecular carrier activitytranscription regulator activitygeneral transcription initiation factor activitymolecular sensor activitymolecular sequestering activityatp-dependent activityother molecular functionall biological processmitotic cell cyclecytokinesiscytoplasmic translationimmune system processmuscle system processcirculatory system processrenal system processrespiratory system processcarbohydrate metabolic processgeneration of precursor metabolites and energydna replicationdna repairdna recombinationchromatin organizationdna-templated transcriptionregulation of dna-templated transcriptiontrna metabolic processprotein foldingprotein glycosylationamino acid metabolic processmodified amino acid metabolic processlipid metabolic processvitamin metabolic processsulfur compound metabolic processintracellular protein transportnucleocytoplasmic transportautophagyinflammatory responsemitochondrion organizationcytoskeleton organizationmicrotubule-based movementperoxisome organizationlysosome organizationchromosome segregationcell adhesionestablishment or maintenance of cell polarityprogrammed cell deathphotosynthesismrna metabolic processsnrna metabolic processvesicle-mediated transportreproductive processdigestive system processsignalingcell differentiationprotein catabolic processextracellular matrix organizationregulatory ncrna-mediated gene silencingtelomere organizationcell junction organizationwound healingribosome biogenesiscilium organizationanatomical structure developmentcell motilitynervous system processendocrine processprotein maturationtransmembrane transportnucleobase-containing small molecule metabolic processhepaticobiliary system processmembrane organizationprotein-containing complex assemblycell wall organization or biogenesisnitrogen cycle metabolic processprotein localization to plasma membranedefense response to other organismdetoxificationmeiotic nuclear divisionmitotic nuclear divisionmitochondrial gene expressioncarbohydrate derivative metabolic processother biological processall cellular componentnuclear chromosomeextracellular regionextracellular spacecell wallnucleusnuclear envelopenucleoplasmchromosomenucleolusmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuslipid dropletmicrotubule organizing centercytosolribosomecytoskeletonplasma membraneciliumplastidthylakoidexternal encapsulating structureextracellular matrixcytoplasmic vesicleorganelleother cellular component
Cell color indicative of number of GO terms
AspectTerm
Cellular Componentnucleus
Molecular Functionidentical protein binding
Biological Process2-micrometer plasmid partitioning
Biological Processprotein stabilization

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    Partitioning protein REP2
  • Short names
    R2
  • Alternative names
    • Protein Charlie
    • Trans-acting factor C

Gene names

    • Name
      REP2
    • Ordered locus names
      R0040C

Encoded on

  • Plasmid 2-micron

Organism names

Accessions

  • Primary accession
    P03872

Proteomes

Organism-specific databases

Subcellular Location

Nucleus
Note: Colocalizes with the STB locus of the 2-micron plasmid as foci in the nucleus near the spindle pole body. Is expelled from STB during a short interval between late G1 and early S phases.

Keywords

PTM/Processing

Features

Showing features for modified residue, chain.

TypeIDPosition(s)Description
Modified residue1N-acetylmethionine
ChainPRO_00001508981-296Partitioning protein REP2

Keywords

Proteomic databases

PTM databases

Interaction

Subunit

Interacts with REP1.

Binary interactions

TypeEntry 1Entry 2Number of experimentsIntact
BINARY P03872REP1 P038717EBI-2125362, EBI-14929
BINARY P03872REP2 P038725EBI-2125362, EBI-2125362

Protein-protein interaction databases

Miscellaneous

Family & Domains

Features

Showing features for region, compositional bias.

TypeIDPosition(s)Description
Region1-57Interaction with REP1
Region58-296DNA-binding, and self-association
Compositional bias228-256Basic and acidic residues
Region228-296Disordered
Region276-296Nuclear localization
Compositional bias277-296Basic residues

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    296
  • Mass (Da)
    33,196
  • Last updated
    1986-07-21 v1
  • Checksum
    FF54CBC6B90F5FAF
MDDIETAKNLTVKARTAYSVWDVCRLFIEMIAPDVDIDIESKRKSDELLFPGYVIRPMESLTTGRPYGLDSSAEDSSVSSDSSAEVILPAAKMVKERFDSIGNGMLSSQEASQAAIDLMLQNNKLLDNRKQLYKSIAIIIGRLPEKDKKRATEMLMRKMDCTQLLVPPAPTEEDVMKLVSVVTQLLTLVPPDRQAALIGDLFIPESLKDIFNSFNELAAENRLQQKKSELEGRTEVNHANTNEEVPSRRTRSRDTNARGAYKLQNTITEGPKAVPTKKRRVATRVRGRKSRNTSRV

Features

Showing features for compositional bias.

TypeIDPosition(s)Description
Compositional bias228-256Basic and acidic residues
Compositional bias277-296Basic residues

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
J01347
EMBL· GenBank· DDBJ
AAB59343.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

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