P03706 · ENLYS_LAMBD
- ProteinEndolysin
- GeneR
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids158 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Endolysin with transglycosylase activity that degrades host peptidoglycans and participates with the holin and spanin proteins in the sequential events which lead to the programmed host cell lysis releasing the mature viral particles. Once the holin has permeabilized the host cell membrane, the endolysin can reach the periplasm and break down the peptidoglycan layer.
Catalytic activity
Activity regulation
Inactivated by zinc.
pH Dependence
Optimum pH is 6.6.
Features
Showing features for active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Active site | 19 | |||||
Sequence: E |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | host cell cytoplasm | |
Molecular Function | lysozyme activity | |
Molecular Function | lytic transglycosylase activity | |
Biological Process | defense response to bacterium | |
Biological Process | peptidoglycan catabolic process | |
Biological Process | viral release from host cell by cytolysis |
Keywords
- Molecular function
- Biological process
Enzyme and pathway databases
Protein family/group databases
Names & Taxonomy
Protein names
- Recommended nameEndolysin
- EC number
- Alternative names
Gene names
Organism names
- Taxonomic lineageViruses > Duplodnaviria > Heunggongvirae > Uroviricota > Caudoviricetes > Lambdavirus > Lambdavirus lambda
- Virus hosts
Accessions
- Primary accessionP03706
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Note: The endolysin is cytoplasmic, but can reach the periplasmic space with the help of the holins which disrupt the host cell membrane.
Keywords
- Cellular component
Phenotypes & Variants
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 31 | No effect on lytic activity. | ||||
Sequence: H → D | ||||||
Mutagenesis | 31 | No effect on lytic activity; when associated with N-137. | ||||
Sequence: H → N | ||||||
Mutagenesis | 48 | 97% loss of lytic activity. | ||||
Sequence: H → N | ||||||
Mutagenesis | 137 | No effect on lytic activity; when associated with N-31. | ||||
Sequence: H → N |
Chemistry
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000218108 | 1-158 | Endolysin | |||
Sequence: MVEINNQRKAFLDMLAWSEGTDNGRQKTRNHGYDVIVGGELFTDYSDHPRKLVTLNPKLKSTGAGRYQLLSRWWDAYRKQLGLKDFSPKSQDAVALQQIKERGALPMIDRGDIRQAIDRCSNIWASLPGAGYGQFEHKADSLIAKFKEAGGTVREIDV |
Interaction
Structure
Sequence
- Sequence statusComplete
- Length158
- Mass (Da)17,825
- Last updated1986-07-21 v1
- Checksum94F8E9A512EAA03E
Features
Showing features for sequence conflict.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Sequence conflict | 74 | in Ref. 2; AA sequence | ||||
Sequence: Missing |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
J02459 EMBL· GenBank· DDBJ | AAA96598.1 EMBL· GenBank· DDBJ | Genomic DNA |