P03518 · GP_RVFV

Function

function

Glycoprotein N

Structural component of the virion that interacts with glycoprotein C (By similarity).
It shields the hydrophobic fusion loops of the glycoprotein C, preventing premature fusion (By similarity).
The glycoprotein protrusions are arranged on an icosahedral lattice, with T=12 triangulation (PubMed:19193794, PubMed:23319635).
They are able to attach the virion to the host cell receptor CD209/DC-SIGN and to promote fusion of membranes with the late endosome after endocytosis of the virion (By similarity).
Plays a role in the packaging of ribonucleoproteins and polymerase during virus assembly (By similarity).

Glycoprotein C

Structural component of the virion that interacts with glycoprotein N (By similarity).
Acts as a class II fusion protein that is activated upon acidification and subsequent repositioning of the glycoprotein N (PubMed:23319635, PubMed:29097548).
The glycoprotein protrusions are arranged on an icosahedral lattice, with T=12 triangulation (PubMed:19193794, PubMed:23319635).
They are able to attach the virion to the host cell receptor CD209/DC-SIGN and to promote fusion of membranes with the late endosome after endocytosis of the virion (By similarity).

Isoform NSm protein

Plays a role for virus dissemination in the mosquito.

NSm-Gn protein

Plays a role for virus dissemination in mosquitoes.

Features

Showing features for site.

TypeIDPosition(s)Description
Site153-154Cleavage; by host signal peptidase
Site690-691Cleavage; by host signal peptidase

GO annotations

AspectTerm
Cellular Componenthost cell endoplasmic reticulum membrane
Cellular Componenthost cell Golgi membrane
Cellular Componenthost cell mitochondrial outer membrane
Cellular Componentmembrane
Cellular Componentvirion membrane
Biological Processentry receptor-mediated virion attachment to host cell
Biological Processfusion of virus membrane with host endosome membrane
Biological Processsymbiont entry into host cell

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    Envelopment polyprotein
  • Alternative names
    • M polyprotein
  • Cleaved into 3 chains

Gene names

    • Name
      GP

Organism names

Accessions

  • Primary accession
    P03518
  • Secondary accessions
    • Q86494
    • Q86495

Subcellular Location

Glycoprotein N

Virion membrane
; Single-pass type I membrane protein
Host Golgi apparatus membrane
; Single-pass type I membrane protein
Host endoplasmic reticulum membrane
; Single-pass type I membrane protein
Note: Interaction between Glycoprotein N and Glycoprotein C is essential for proper targeting of Glycoprotein C to the Golgi complex, where virion budding occurs.

Glycoprotein C

Virion membrane
; Single-pass type I membrane protein
Host Golgi apparatus membrane
; Single-pass type I membrane protein
Note: Interaction between Glycoprotein N and Glycoprotein C is essential for proper targeting of Glycoprotein C to the Golgi complex, where virion budding occurs.

Isoform NSm protein

Host mitochondrion outer membrane
; Single-pass type II membrane protein

Features

Showing features for topological domain, transmembrane.

TypeIDPosition(s)Description
Topological domain17-130Cytoplasmic
Topological domain154-582Lumenal
Transmembrane583-603Helical
Topological domain604-673Cytoplasmic
Topological domain691-1159Lumenal
Transmembrane1160-1180Helical
Topological domain1181-1206Cytoplasmic

Keywords

PTM/Processing

Features

Showing features for signal, chain, disulfide bond, glycosylation.

TypeIDPosition(s)Description
Signal1-16
ChainPRO_000003684717-690NSm-Gn protein
ChainPRO_000024700917-1206Envelopment polyprotein
ChainPRO_0000036848154-690Glycoprotein N
Disulfide bond179↔188
Disulfide bond229↔239
Disulfide bond250↔281
Disulfide bond271↔284
Disulfide bond304↔456
Disulfide bond322↔332
Disulfide bond374↔434
Disulfide bond402↔413
Disulfide bond420↔425
Disulfide bond479↔482
Disulfide bond486↔556
Disulfide bond506↔511
Disulfide bond691↔731
ChainPRO_0000036849691-1206Glycoprotein C
Disulfide bond704↔713
Disulfide bond756↔852
Disulfide bond771↔965
Disulfide bond777↔825
Disulfide bond783↔832
Disulfide bond788↔814
Glycosylation794N-linked (GlcNAc...) asparagine; by host
Disulfide bond818↔823
Disulfide bond934↔947
Disulfide bond1029↔1101
Glycosylation1035N-linked (GlcNAc...) asparagine; by host
Disulfide bond1039↔1042
Disulfide bond1049↔1083
Glycosylation1077N-linked (GlcNAc...) asparagine; by host

Post-translational modification

Envelopment polyprotein

Specific enzymatic cleavages in vivo yield mature proteins including NSm protein, Glycoprotein C, and Glycoprotein N.

Glycoprotein N

Glycosylated (By similarity).
The glycans can attach to host CD209/DC-SIGN, and may play a role in virus entry into dendritic cells (By similarity).

Glycoprotein C

Glycosylated (By similarity).
The glycans can attach to host CD209/DC-SIGN, and may play a role in virus entry into dendritic cells (By similarity).

Glycoprotein C

Palmitoylated.

Keywords

PTM databases

Interaction

Subunit

Glycoprotein N

Heterodimer with glycoprotein C (PubMed:19193794, PubMed:28827346).
Interacts with nucleocapsid protein N and with the polymerase L in order to package them into virus particles (By similarity).

Glycoprotein C

Heterodimer with glycoprotein C (PubMed:19193794, PubMed:28827346).
Homotrimer (postfusion) (PubMed:29097548).
Interacts with nucleocapsid protein N and with the polymerase L in order to package them into virus particles (By similarity).
Interacts with host E3 ubiquitin-protein ligase UBR4; this interaction is important for viral RNA production (By similarity).
Interacts with host LRP1; this interaction facilitates virus entry into the host cell (By similarity).

Structure

Family & Domains

Features

Showing features for region.

TypeIDPosition(s)Description
Region131-153Internal signal sequence for glycoprotein N
Region608-650Golgi retention signal
Region646-650Important for correct targeting of the glycoproteins to the Golgi complex but not for heterodimerization
Region675-690Internal signal sequence for glycoprotein C
Region777-783Fusion loop
Region819-830Fusion loop

Domain

Glycoprotein N

Contains a Golgi retention signal on its C-terminus (By similarity).
The cytoplasmic tail specifically interacts with the ribonucleoproteins and is critical for genome packaging (By similarity).

Sequence similarities

Keywords

Family and domain databases

Sequence & Isoforms

Align isoforms (3)
  • Sequence status
    Complete
  • Sequence processing
    The displayed sequence is further processed into a mature form.

This entry describes 3 isoforms produced by Alternative initiation.

P03518-1

This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

  • Length
    1,206
  • Mass (Da)
    132,053
  • Last updated
    1995-11-01 v2
  • Checksum
    D2E8017179285924
MYVLLTILISVLVCEAVIRVSLSSTREETCFGDSTNPEMIEGAWDSLREEEMPEELSCSISGIREVKTSSQELYRALKAIIAADGLNNITCHGKDPEDKISLIKGPPHKKRVGIVRCERRRDAKQIGRETMAGIAMTVLPALAVFALAPVVFAEDPHLRNRPGKGHNYIDGMTQEDATCKPVTYAGACSSFDVLLEKGKFPLFQSYAHHRTLLEAVHDTIIAKADPPSCDLQSAHGNPCMKEKLVMKTHCPNDYQSAHYLNNDGKMASVKCPPKYGLTEDCNFCRQMTGASLKKGSYPLQDLFCQSSEDDGSKLKTKMKGVCEVGVQAHKKCDGQLSTAHEVVPFAVFKNSKKVYLDKLDLKTEENLLPDSFVCFEHKGQYKGTMDSGQTKRELKSFDISQCPKIGGHGSKKCTGDAAFCSAYECTAQYANAYCSHANGSGIVQIQVSGVWKKPLCVGYERVVVKRELSAKPIQRVEPCTTCITKCEPHGLVVRSTGFKISSAVACASGVCVTGSQSPSTEITLKYPGISQSSGGDIGVHMAHDDQSVSSKIVAHCPPQDPCLVHGCIVCAHGLINYQCHTALSAFVVVFVFSSIAIICLAVLYRVLKCLKIAPRKVLNPLMWITAFIRWIYKKMVARVAHNINQVNREIGWMEGGQLVLGNPAPIPRHAPIPRYSTYLMLLLIVSYASACSELIQASSRITTCSTEGVNTKCRLSGTALIRAGSVGAEACLMLKGVKEDQTKFLKIKTVSSELSCREGQSYWTGSISPKCLSSRRCHLVGECHVNRCLSWRDNETSAEFSFVGESTTMRENKCFEQCGGWGCGCFNVNPSCLFVHTYLQSVRKEALRVFNCIDWVHKLTLEITDFDGSVSTIDLGASSSRFTNWGSVSLSLDAEGISGSNSFSFIESPSKGYAIVDEPFSEIPRQGFLGEIRCNSESSVLSAHESCLRAPNLISYKPMIDQLECTTNLIDPFVVFERGSLPQTRNDKTFAASKGNRGVQAFSKGSVQADLTLMFDNFEVDFVGAAVSCDAAFLNLTGCYSCNAGARVCLSITSTGTGSLSAHNKDGSLHIVLPSENGTKDQCQILHFTVPEVEEEFMYSCDGDERPLLVKGTLIAIDPFDDRREAGGESTVVNPKSGSWNFFDWFSGLMSWFGGPLKLYSSFACMLHYQLGSFSSLYILEEQASLKCGLLPLRRPHRSVRVKVIC

P03518-2

P03518-3

Features

Showing features for alternative sequence.

TypeIDPosition(s)Description
Alternative sequenceVSP_0579861-38in isoform NSm protein
Alternative sequenceVSP_0579851-51in isoform NSm' protein
Alternative sequenceVSP_057987154-1197in isoform NSm protein and isoform NSm' protein

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
M11157
EMBL· GenBank· DDBJ
AAA47450.1
EMBL· GenBank· DDBJ
Genomic RNA

Similar Proteins

Disclaimer

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