P03517 · GP_PTPV

  • Protein
    Envelopment polyprotein
  • Gene
    GP
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    5/5

Function

function

Glycoprotein N

Structural component of the virion that interacts with glycoprotein C (By similarity).
It shields the hydrophobic fusion loops of the glycoprotein C, preventing premature fusion (By similarity).
The glycoprotein protrusions are arranged on an icosahedral lattice, with T=12 triangulation (By similarity).
They are able to attach the virion to the host cell receptor CD209/DC-SIGN and to promote fusion of membranes with the late endosome after endocytosis of the virion (PubMed:21767814).
Plays a role in the packaging of ribonucleoproteins and polymerase during virus assembly (By similarity).

Glycoprotein C

Structural component of the virion that interacts with glycoprotein N (By similarity).
Acts as a class II fusion protein that is activated upon acidification and subsequent repositioning of the glycoprotein N. The glycoprotein protrusions are arranged on an icosahedral lattice, with T=12 triangulation (By similarity).
They are able to attach the virion to the host cell receptor CD209/DC-SIGN and to promote fusion of membranes with the late endosome after endocytosis of the virion (PubMed:21767814).

NSm-Gn protein

Plays a role for virus dissemination in mosquitoes.

Features

Showing features for site.

TypeIDPosition(s)Description
Site271-272Cleavage; by host signal peptidase
Site809-810Cleavage; by host signal peptidase
Site1311Important for glycoprotein C and glycoprotein N subcellular location

GO annotations

AspectTerm
Cellular Componenthost cell endoplasmic reticulum membrane
Cellular Componenthost cell Golgi membrane
Cellular Componentmembrane
Cellular Componentvirion membrane
Biological Processentry receptor-mediated virion attachment to host cell
Biological Processfusion of virus membrane with host endosome membrane
Biological Processsymbiont entry into host cell

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    Envelopment polyprotein
  • Alternative names
    • M polyprotein
  • Cleaved into 3 chains

Gene names

    • Name
      GP

Organism names

Accessions

  • Primary accession
    P03517

Subcellular Location

Glycoprotein N

Virion membrane
; Single-pass type I membrane protein
Host Golgi apparatus membrane
; Single-pass type I membrane protein
Host endoplasmic reticulum membrane
; Single-pass type I membrane protein
Note: Interaction between Glycoprotein N and Glycoprotein C is essential for proper targeting of Glycoprotein C to the Golgi complex, where virion budding occurs.

Glycoprotein C

Virion membrane
; Single-pass type I membrane protein
Host Golgi apparatus membrane
; Single-pass type I membrane protein
Note: Interaction between Glycoprotein N and Glycoprotein C is essential for proper targeting of Glycoprotein C to the Golgi complex, where virion budding occurs.

Features

Showing features for topological domain, transmembrane.

TypeIDPosition(s)Description
Topological domain18-712Lumenal
Transmembrane713-733Helical
Topological domain734-791Cytoplasmic
Topological domain810-1278Lumenal
Transmembrane1279-1299Helical
Topological domain1300-1313Cytoplasmic

Keywords

PTM/Processing

Features

Showing features for signal, chain, glycosylation, disulfide bond.

TypeIDPosition(s)Description
Signal1-17
ChainPRO_000003684418-270NSm-Gn protein
ChainPRO_000024700818-1313Envelopment polyprotein
Glycosylation76N-linked (GlcNAc...) asparagine; by host
Glycosylation102N-linked (GlcNAc...) asparagine; by host
ChainPRO_0000036845271-809Glycoprotein N
Disulfide bond303↔312
Disulfide bond352↔362
Disulfide bond373↔404
Disulfide bond394↔407
Disulfide bond432↔579
Disulfide bond450↔460
Glycosylation496N-linked (GlcNAc...) asparagine; by host
Disulfide bond501↔557
Disulfide bond525↔536
Disulfide bond543↔548
Disulfide bond602↔605
Disulfide bond609↔679
Disulfide bond629↔634
Disulfide bond810↔850
ChainPRO_0000036846810-1313Glycoprotein C
Disulfide bond823↔832
Disulfide bond875↔971
Disulfide bond890↔1084
Disulfide bond896↔944
Disulfide bond902↔951
Disulfide bond907↔933
Disulfide bond937↔942
Disulfide bond1053↔1066
Disulfide bond1148↔1220
Glycosylation1154N-linked (GlcNAc...) asparagine; by host
Disulfide bond1158↔1161
Disulfide bond1168↔1202
Glycosylation1243N-linked (GlcNAc...) asparagine; by host

Post-translational modification

Envelopment polyprotein

Specific enzymatic cleavages in vivo yield mature proteins including NSm protein, Glycoprotein C, and Glycoprotein N.

Glycoprotein N

Glycosylated. The glycans can attach to host CD209/DC-SIGN, and may play a role in virus entry into dendritic cells.

Glycoprotein C

Glycosylated. The glycans can attach to host CD209/DC-SIGN, and may play a role in virus entry into dendritic cells.

Glycoprotein C

Palmitoylated.

Keywords

PTM databases

Interaction

Subunit

Glycoprotein N

Heterodimer with glycoprotein C (By similarity).
Interacts with nucleocapsid protein N and with the polymerase L in order to package them into virus particles (By similarity).

Glycoprotein C

Heterodimer with glycoprotein C. Homotrimer (postfusion) (By similarity).
Interacts with nucleocapsid protein N and with the polymerase L in order to package them into virus particles. Interacts with host E3 ubiquitin-protein ligase UBR4; this interaction is important for viral RNA production (By similarity).

Structure

3D structure databases

Family & Domains

Features

Showing features for coiled coil, region.

TypeIDPosition(s)Description
Coiled coil178-237
Region250-270Internal signal sequence for glycoprotein N
Region731-773Golgi retention signal
Region769-773Important for correct targeting of the glycoproteins to the Golgi complex but not for heterodimerization
Region793-809Internal signal sequence for glycoprotein C
Region896-902Fusion loop
Region938-949Fusion loop

Domain

Glycoprotein N

Contains a Golgi retention signal on its C-terminus. The cytoplasmic tail specifically interacts with the ribonucleoproteins and is critical for genome packaging.

Sequence similarities

Keywords

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Sequence processing
    The displayed sequence is further processed into a mature form.
  • Length
    1,313
  • Mass (Da)
    146,375
  • Last updated
    1986-07-21 v1
  • Checksum
    72072F27735A74A2
MIFTILNVLTRAMLVMSMYSLTTWDSTSRNDMCFSNDSPLEGLVYYWETHSKRHDYKKQESQRCRVGDSDKKMITNVTIISLISEIQKSISELSLSCVNDDNSTGQVLTFNGLEDTIRGDYIVDCVTGLYQSDIGVGVGLGRTHHGHQQMKNKAVVIDEKERMISLLETQQSENDIKMQVLMSEIEQLKNQLSKKRNERGQEKRDAEKVMSDLMARNSDLRKHNDILTAEISQMKNKNTIQRNKNTVSTTVVPAILSVALLSSSVAPIIAAPPDSPMINPWPHAKNRVGTGMYKYDENDDSGCRPIRYGVSCIGFDFMLKMDKYPFFNAFIGHKTPLESFADKIIEKEEETCEIGTNKEFKCFEERAYIKGTCPTNINAVHYIDNKGKLRYVKCKENLEMTEDCAFCRKIKKKAGQSVQVQKTSVPLQDAICQENSDTYSGPKIPFKGVCKIGLIKYKECKFKTSSYETVSFITLKEKGKIYIEHLMLKNIEVVTNVSFVCYEHVGQDEQEVEHRALKRVSVNDCKIVDNSKQKICTGDHVFCEKYDCSTSYPDVTCIHAPGSGPLYINLMGSWIKPQCVGYERVLVDREVKQPLLAPEQNCDTCVSECLDEGVHIKSTGFEITSAVACSHGSCISAHQEPSTSVIVPYPGLLASVGGRIGIHLSHTSDSASVHMVVVCPPRDSCAAHNCLLCYHGILNYQCHSTLSAILTSFLLILFIYTVFSVTTNILYVLRLIPKQLKSPVGWLKLFINWLLTALRIKTRNVMRRINQRIGWVDHHDVERPRHREPMRRFKTTLLLTLIMMTGGNACSNTVVANSKQTRCVQEGSNTKCSITATITLRAGVIGAESCFIIKGPMENQQKTISIKTISSETVCREGSSFWTSLYIPSCLSSRRCHLVGDCVGNKCQSWRDDQLSREFSGVKDNHIMNENKCFEQCGAIGCGCFNINPSCLYVHAYLKSARNEAVRVFSCSDWVHRVSFEVKGPDGETELVTLGSPGTKFLNWGTLSLSLDAEGISGTNSISFLESSKGGFALYDEGYNEIPREGFLGEIRCSSESAAISAHKSCIRAPGLIKYKPMTDQIECTASLVDPFAIFLKGSLPQTRNGQTFTSTKDKKTVQAFTNGAIKALLSINLDDHEIVFINKVKNCDATFLNVSGCYSCDYGAHVCVKVKSSESADFFAESEDKTTVLSFPIQSGTHDYCQVLHFQKPLVDERLSYSCGSEPKLIVIKGTLVCMGVYDFRNKTGGSSTVVNPSEGAWSISNWFSGLLDWLGGPMKAILKILGFIAIGIVCFVLFMILIRIAVNSINIKKKN

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
M11156
EMBL· GenBank· DDBJ
AAA47110.1
EMBL· GenBank· DDBJ
Genomic RNA

Similar Proteins

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