P03252 · PRO_ADE02
- ProteinProtease
- GeneL3
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids204 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Cleaves viral precursor proteins (pTP, pIIIa, pVI, pVII, pVIII, and pX) inside newly assembled particles giving rise to mature virions. Protease complexed to its cofactor slides along the viral DNA to specifically locate and cleave the viral precursors. Mature virions have a weakened organization compared to the unmature virions, thereby facilitating subsequent uncoating. Without maturation, the particle lacks infectivity and is unable to uncoat. Late in adenovirus infection, in the cytoplasm, may participate in the cytoskeleton destruction. Cleaves host cell cytoskeletal keratins K7 and K18.
Miscellaneous
All late proteins expressed from the major late promoter are produced by alternative splicing and alternative polyadenylation of the same gene giving rise to non-overlapping ORFs. A leader sequence is present in the N-terminus of all these mRNAs and is recognized by the viral shutoff protein to provide expression although conventional translation via ribosome scanning from the cap has been shut off in the host cell.
Catalytic activity
Activity regulation
Requires DNA and protease cofactor for maximal activation. Inside nascent virions, becomes partially activated by binding to the viral DNA, allowing it to cleave the cofactor that binds to the protease and fully activates it. Actin, like the viral protease cofactor, seems to act as a cofactor in the cleavage of cytokeratin 18 and of actin itself.
Features
Showing features for site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Site | 51-52 | Cleavage; by autolysis | ||||
Sequence: GG | ||||||
Active site | 54 | |||||
Sequence: H | ||||||
Active site | 71 | |||||
Sequence: E | ||||||
Active site | 122 | |||||
Sequence: C |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | host cell nucleus | |
Cellular Component | virion component | |
Molecular Function | cysteine-type endopeptidase activity | |
Molecular Function | cysteine-type peptidase activity | |
Molecular Function | DNA binding | |
Biological Process | proteolysis |
Keywords
- Molecular function
Enzyme and pathway databases
Protein family/group databases
Names & Taxonomy
Protein names
- Recommended nameProtease
- EC number
- Alternative names
Gene names
Organism names
- Taxonomic lineageViruses > Varidnaviria > Bamfordvirae > Preplasmiviricota > Tectiliviricetes > Rowavirales > Adenoviridae > Mastadenovirus > Human mastadenovirus C
- Virus hosts
Accessions
- Primary accessionP03252
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Note: Present in about 10 copies per virion.
Keywords
- Cellular component
Phenotypes & Variants
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 137 | Loss of activity; gives rise to unprocessed capsids defective in endosomal escape. | ||||
Sequence: P → L |
PTM/Processing
Features
Showing features for chain, disulfide bond.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000218023 | 1-204 | Protease | |||
Sequence: MGSSEQELKAIVKDLGCGPYFLGTYDKRFPGFVSPHKLACAIVNTAGRETGGVHWMAFAWNPRSKTCYLFEPFGFSDQRLKQVYQFEYESLLRRSAIASSPDRCITLEKSTQSVQGPNSAACGLFCCMFLHAFANWPQTPMDHNPTMNLITGVPNSMLNSPQVQPTLRRNQEQLYSFLERHSPYFRSHSAQIRSATSFCHLKNM | ||||||
Disulfide bond | 104 | Interchain (with C-10 in cleaved protease cofactor pVI-C) | ||||
Sequence: C |
Keywords
- PTM
Expression
Induction
Expressed in the late phase of the viral replicative cycle.
Keywords
- Developmental stage
Interaction
Subunit
Interacts with protease cofactor pVI-C; this interaction is necessary for protease activation.
Structure
Sequence
- Sequence statusComplete
- Length204
- Mass (Da)23,087
- Last updated1986-07-21 v1
- ChecksumE43C7DDF14A589A2
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
J01917 EMBL· GenBank· DDBJ | AAA92216.1 EMBL· GenBank· DDBJ | Genomic DNA |