P03129 · VE7_HPV16
- ProteinProtein E7
- GeneE7
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Plays a role in viral genome replication by driving entry of quiescent cells into the cell cycle. Stimulation of progression from G1 to S phase allows the virus to efficiently use the cellular DNA replicating machinery to achieve viral genome replication. E7 protein has both transforming and trans-activating activities. Induces the disassembly of the E2F1 transcription factor from RB1, with subsequent transcriptional activation of E2F1-regulated S-phase genes. Interferes with host histone deacetylation mediated by HDAC1 and HDAC2, leading to transcription activation. Also plays a role in the inhibition of both antiviral and antiproliferative functions of host interferon alpha. Interaction with host TMEM173/STING impairs the ability of TMEM173/STING to sense cytosolic DNA and promote the production of type I interferon (IFN-alpha and IFN-beta).
Miscellaneous
HPV16, in comparison to HPV types 6 and 11, is more often associated with malignant genital cancers in humans.
Part of the antigens expressed and presented within HPV-positive head and neck tumors.
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Keywords
- Molecular function
- Biological process
- Ligand
Names & Taxonomy
Protein names
- Recommended nameProtein E7
Gene names
Organism names
- Organism
- Strains
- Taxonomic lineageViruses > Monodnaviria > Shotokuvirae > Cossaviricota > Papovaviricetes > Zurhausenvirales > Papillomaviridae > Firstpapillomavirinae > Alphapapillomavirus > Alphapapillomavirus 9
- Virus hosts
Accessions
- Primary accessionP03129
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Note: Predominantly found in the host nucleus.
Keywords
- Cellular component
Phenotypes & Variants
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 67 | Complete loss of interaction with HDAC1 and transformation ability. | ||||
Sequence: L → R | ||||||
Mutagenesis | 82-83 | Complete loss of interaction with HDAC1 and transformation ability. | ||||
Sequence: LL → RR | ||||||
Mutagenesis | 91 | Complete loss of interaction with HDAC1 and transformation ability. | ||||
Sequence: C → G |
Keywords
- Disease
Chemistry
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000133414 | 1-98 | Protein E7 | |||
Sequence: MHGDTPTLHEYMLDLQPETTDLYCYEQLNDSSEEEDEIDGPAGQAEPDRAHYNIVTFCCKCDSTLRLCVQSTHVDIRTLEDLLMGTLGIVCPICSQKP |
Post-translational modification
Highly phosphorylated.
PTM databases
Expression
Keywords
- Developmental stage
Interaction
Subunit
Homodimer (PubMed:11123931).
Homooligomer (PubMed:15035602).
Interacts with host RB1; this interaction induces dissociation of RB1-E2F1 complex thereby disrupting RB1 activity (PubMed:1316611).
Interacts with host EP300; this interaction represses EP300 transcriptional activity (PubMed:12970734).
Forms a complex with CHD4 and HDAC1, thereby altering the action of host histone deacetylation. A similar complex involving E7, CHD4 and HDAC2 may also form (PubMed:10228159).
Interacts with protein E2; this interaction inhibits E7 oncogenic activity (PubMed:16439535).
Homooligomer (PubMed:15035602).
Interacts with host RB1; this interaction induces dissociation of RB1-E2F1 complex thereby disrupting RB1 activity (PubMed:1316611).
Interacts with host EP300; this interaction represses EP300 transcriptional activity (PubMed:12970734).
Forms a complex with CHD4 and HDAC1, thereby altering the action of host histone deacetylation. A similar complex involving E7, CHD4 and HDAC2 may also form (PubMed:10228159).
Interacts with protein E2; this interaction inhibits E7 oncogenic activity (PubMed:16439535).
Binary interactions
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for region, motif, zinc finger.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 1-40 | E7 terminal domain | ||||
Sequence: MHGDTPTLHEYMLDLQPETTDLYCYEQLNDSSEEEDEIDG | ||||||
Motif | 22-26 | LXCXE motif; interaction with host RB1 and TMEM173/STING | ||||
Sequence: LYCYE | ||||||
Zinc finger | 58-94 | |||||
Sequence: CCKCDSTLRLCVQSTHVDIRTLEDLLMGTLGIVCPIC | ||||||
Motif | 76-84 | Nuclear export signal | ||||
Sequence: IRTLEDLLM |
Domain
The E7 terminal domain is an intrinsically disordered domain, whose flexibility and conformational transitions confer target adaptability to the oncoprotein. It allows adaptation to a variety of protein targets and exposes the PEST degradation sequence that regulates its turnover in the cell.
Sequence similarities
Belongs to the papillomaviridae E7 protein family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length98
- Mass (Da)11,022
- Last updated1986-07-21 v1
- Checksum9BD612534CD2C9EB
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
K02718 EMBL· GenBank· DDBJ | AAA46940.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
D00735 EMBL· GenBank· DDBJ | BAA00633.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AF003020 EMBL· GenBank· DDBJ | AAB70737.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AF003023 EMBL· GenBank· DDBJ | AAB70740.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AF003024 EMBL· GenBank· DDBJ | AAB70741.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AF003025 EMBL· GenBank· DDBJ | AAB70742.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AF003026 EMBL· GenBank· DDBJ | AAB70743.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
U76411 EMBL· GenBank· DDBJ | AAB18962.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
U76412 EMBL· GenBank· DDBJ | AAB18963.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
U76413 EMBL· GenBank· DDBJ | AAB18964.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AF536179 EMBL· GenBank· DDBJ | AAQ10713.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AF536180 EMBL· GenBank· DDBJ | AAQ10721.1 EMBL· GenBank· DDBJ | Genomic DNA |