P03023 · LACI_ECOLI

Function

function

Repressor of the lactose operon. Binds allolactose as an inducer.

Miscellaneous

Removing residues 1-59 results in loss of DNA-binding activity but retains tetrameric structure and inducer-binding activity. Deleting residues 340-360 results in loss of tetramer formation, but retains dimer formation, inducer-binding activity, and DNA-binding activity (if residues 1-59 are present).

Features

Showing features for dna binding.

136050100150200250300350
TypeIDPosition(s)Description
DNA binding6-25H-T-H motif

GO annotations

all annotationsall molecular functionvirus receptor activitydna bindingrna bindingcytoskeletal motor activitycatalytic activitygtpase activitystructural molecule activitytransporter activitycytoskeletal protein bindinglipid bindingcyclase activityantioxidant activityoxidoreductase activitytransferase activityhydrolase activitylyase activityisomerase activityligase activityprotein tag activitycargo receptor activityhistone bindingprotein folding chaperonetranslation regulator activitynutrient reservoir activityreceptor ligand activitymolecular transducer activitymolecular adaptor activitytoxin activitycell adhesion mediator activitymolecular function regulator activityvirus coreceptor activitycatalytic activity, acting on a proteincatalytic activity, acting on dnacatalytic activity, acting on rnamolecular carrier activitytranscription regulator activitygeneral transcription initiation factor activitymolecular sensor activitymolecular sequestering activityatp-dependent activityother molecular functionall biological processmitotic cell cyclecytokinesiscytoplasmic translationimmune system processmuscle system processcirculatory system processrenal system processrespiratory system processcarbohydrate metabolic processgeneration of precursor metabolites and energydna replicationdna repairdna recombinationchromatin organizationdna-templated transcriptionregulation of dna-templated transcriptiontrna metabolic processprotein foldingprotein glycosylationamino acid metabolic processmodified amino acid metabolic processlipid metabolic processvitamin metabolic processsulfur compound metabolic processintracellular protein transportnucleocytoplasmic transportautophagyinflammatory responsemitochondrion organizationcytoskeleton organizationmicrotubule-based movementperoxisome organizationlysosome organizationchromosome segregationcell adhesionestablishment or maintenance of cell polarityprogrammed cell deathphotosynthesismrna metabolic processsnrna metabolic processvesicle-mediated transportreproductive processdigestive system processsignalingcell differentiationprotein catabolic processextracellular matrix organizationregulatory ncrna-mediated gene silencingtelomere organizationcell junction organizationwound healingribosome biogenesiscilium organizationanatomical structure developmentcell motilitynervous system processendocrine processprotein maturationtransmembrane transportnucleobase-containing small molecule metabolic processhepaticobiliary system processmembrane organizationprotein-containing complex assemblycell wall organization or biogenesisnitrogen cycle metabolic processprotein localization to plasma membranedefense response to other organismdetoxificationmeiotic nuclear divisionmitotic nuclear divisionmitochondrial gene expressioncarbohydrate derivative metabolic processother biological processall cellular componentnuclear chromosomeextracellular regionextracellular spacecell wallnucleusnuclear envelopenucleoplasmchromosomenucleolusmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuslipid dropletmicrotubule organizing centercytosolribosomecytoskeletonplasma membraneciliumplastidthylakoidexternal encapsulating structureextracellular matrixcytoplasmic vesicleorganelleother cellular component
Cell color indicative of number of GO terms
AspectTerm
Cellular Componentcytosol
Molecular Functioncis-regulatory region sequence-specific DNA binding
Molecular FunctionDNA-binding transcription factor activity
Molecular FunctionDNA-binding transcription repressor activity
Molecular Functionidentical protein binding
Molecular Functiontranscription cis-regulatory region binding
Biological Processnegative regulation of DNA-templated transcription
Biological Processregulation of DNA-templated transcription

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Lactose operon repressor

Gene names

    • Name
      lacI
    • Ordered locus names
      b0345, JW0336

Organism names

  • Taxonomic identifier
  • Strains
    • K12 / MG1655 / ATCC 47076
    • K12 / W3110 / ATCC 27325 / DSM 5911
  • Taxonomic lineage
    Bacteria > Pseudomonadota > Gammaproteobacteria > Enterobacterales > Enterobacteriaceae > Escherichia

Accessions

  • Primary accession
    P03023
  • Secondary accessions
    • O09196
    • P71309
    • Q2MC79
    • Q47338

Proteomes

Subcellular Location

Phenotypes & Variants

Features

Showing features for mutagenesis, natural variant.

TypeIDPosition(s)Description
Mutagenesis17Broadening of specificity.
Mutagenesis22Recognizes an operator variant.
Natural variant282in T41 mutant

Variants

We now provide the "Disease & Variants" viewer in its own tab.

The viewer provides 1 variant from UniProt as well as other sources including ClinVar and dbSNP.

Go to variant viewer

Chemistry

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00001079631-360Lactose operon repressor

Proteomic databases

Interaction

Subunit

Homotetramer.

Binary interactions

TypeEntry 1Entry 2Number of experimentsIntact
BINARY P03023lacI P030233EBI-909231, EBI-909231

Protein-protein interaction databases

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain1-58HTH lacI-type

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    360
  • Mass (Da)
    38,590
  • Last updated
    2003-07-19 v3
  • Checksum
    347A8DEE92D736CB
MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ

Sequence caution

The sequence AAB18069.1 differs from that shown. Reason: Erroneous initiation
The sequence AAB47270.1 differs from that shown. Reason: Erroneous initiation

Features

Showing features for sequence conflict.

TypeIDPosition(s)Description
Sequence conflict286in Ref. 1, 4 and 7

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
V00294
EMBL· GenBank· DDBJ
CAA23569.1
EMBL· GenBank· DDBJ
Genomic DNA
X58469
EMBL· GenBank· DDBJ
CAA41383.1
EMBL· GenBank· DDBJ
Genomic DNA
U86347
EMBL· GenBank· DDBJ
AAB47270.1
EMBL· GenBank· DDBJ
Genomic DNA Different initiation
J01636
EMBL· GenBank· DDBJ
AAA24052.1
EMBL· GenBank· DDBJ
Genomic DNA
U72488
EMBL· GenBank· DDBJ
AAB36549.1
EMBL· GenBank· DDBJ
Genomic DNA
U78872
EMBL· GenBank· DDBJ
AAB37348.1
EMBL· GenBank· DDBJ
Genomic DNA
U78873
EMBL· GenBank· DDBJ
AAB37351.1
EMBL· GenBank· DDBJ
Genomic DNA
U78874
EMBL· GenBank· DDBJ
AAB37354.1
EMBL· GenBank· DDBJ
Genomic DNA
U73857
EMBL· GenBank· DDBJ
AAB18069.1
EMBL· GenBank· DDBJ
Genomic DNA Different initiation
U00096
EMBL· GenBank· DDBJ
AAC73448.1
EMBL· GenBank· DDBJ
Genomic DNA
AP009048
EMBL· GenBank· DDBJ
BAE76127.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

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