P02992 · EFTU_YEAST
- ProteinElongation factor Tu, mitochondrial
- GeneTUF1
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids437 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
G-protein that, in its active GTP-bound form, binds to and delivers aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. In the presence of a correct codon-anticodon match between the aminoacyl-tRNA and the A-site codon of the ribosome-bound mRNA, the ribosome acts as a GTPase activator and the GTP is hydrolyzed. The inactive GDP-bound form leaves the ribosome and must be recycled before binding another molecule of aminoacyl-tRNA. Required for mitochondrial protein biosynthesis and maintenance of mitochondrial DNA.
Miscellaneous
Present with 58527 molecules/cell in log phase SD medium.
Pathway
Protein biosynthesis; polypeptide chain elongation.
Features
Showing features for binding site.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | mitochondrion | |
Molecular Function | GTP binding | |
Molecular Function | GTPase activity | |
Molecular Function | translation elongation factor activity | |
Biological Process | mitochondrial translation | |
Biological Process | mitochondrial translational elongation |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameElongation factor Tu, mitochondrial
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Fungi > Dikarya > Ascomycota > Saccharomycotina > Saccharomycetes > Saccharomycetales > Saccharomycetaceae > Saccharomyces
Accessions
- Primary accessionP02992
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
PTM/Processing
Features
Showing features for transit peptide, chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Transit peptide | 1-38 | Mitochondrion | ||||
Sequence: MSALLPRLLTRTAFKASGKLLRLSSVISRTFSQTTTSY | ||||||
Chain | PRO_0000007465 | 39-437 | Elongation factor Tu, mitochondrial | |||
Sequence: AAAFDRSKPHVNIGTIGHVDHGKTTLTAAITKTLAAKGGANFLDYAAIDKAPEERARGITISTAHVEYETAKRHYSHVDCPGHADYIKNMITGAAQMDGAIIVVAATDGQMPQTREHLLLARQVGVQHIVVFVNKVDTIDDPEMLELVEMEMRELLNEYGFDGDNAPIIMGSALCALEGRQPEIGEQAIMKLLDAVDEYIPTPERDLNKPFLMPVEDIFSISGRGTVVTGRVERGNLKKGEELEIVGHNSTPLKTTVTGIEMFRKELDSAMAGDNAGVLLRGIRRDQLKRGMVLAKPGTVKAHTKILASLYILSKEEGGRHSGFGENYRPQMFIRTADVTVVMRFPKEVEDHSMQVMPGDNVEMECDLIHPTPLEVGQRFNIREGGRTVGTGLITRIIE |
Post-translational modification
The precursor is processed in two steps involving mitochondrial intermediate peptidase (MIP) and mitochondrial processing peptidase (MPP).
Proteomic databases
PTM databases
Structure
Family & Domains
Features
Showing features for domain, region.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 46-242 | tr-type G | ||||
Sequence: KPHVNIGTIGHVDHGKTTLTAAITKTLAAKGGANFLDYAAIDKAPEERARGITISTAHVEYETAKRHYSHVDCPGHADYIKNMITGAAQMDGAIIVVAATDGQMPQTREHLLLARQVGVQHIVVFVNKVDTIDDPEMLELVEMEMRELLNEYGFDGDNAPIIMGSALCALEGRQPEIGEQAIMKLLDAVDEYIPTPE | ||||||
Region | 55-62 | G1 | ||||
Sequence: GHVDHGKT | ||||||
Region | 96-100 | G2 | ||||
Sequence: GITIS | ||||||
Region | 117-120 | G3 | ||||
Sequence: DCPG | ||||||
Region | 172-175 | G4 | ||||
Sequence: NKVD | ||||||
Region | 210-212 | G5 | ||||
Sequence: SAL |
Sequence similarities
Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-Tu/EF-1A subfamily.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Sequence processingThe displayed sequence is further processed into a mature form.
- Length437
- Mass (Da)47,972
- Last updated1986-07-21 v1
- Checksum26F4DFE805D1A93B
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
K00428 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. | |
Z75095 EMBL· GenBank· DDBJ | CAA99396.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BK006948 EMBL· GenBank· DDBJ | DAA10959.1 EMBL· GenBank· DDBJ | Genomic DNA |