P02981 · TCR3_ECOLX
- ProteinTetracycline resistance protein, class C
- GenetetA
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids396 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score3/5
Function
function
Resistance to tetracycline by an active tetracycline efflux. This is an energy-dependent process that decreases the accumulation of the antibiotic in whole cells. This protein functions as a metal-tetracycline/H+ antiporter.
Biotechnology
This protein is used as a marker in many commonly used cloning vectors, such as pBR322 and pACYC184.
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | plasma membrane | |
Molecular Function | antiporter activity | |
Biological Process | proton transmembrane transport | |
Biological Process | response to antibiotic |
Keywords
- Biological process
Protein family/group databases
Names & Taxonomy
Protein names
- Recommended nameTetracycline resistance protein, class C
- Short namesTetA(C)
Gene names
Encoded on
- Plasmid pSC101
Organism names
- Organism
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Enterobacterales > Enterobacteriaceae > Escherichia
Accessions
- Primary accessionP02981
Subcellular Location
UniProt Annotation
GO Annotation
Cell inner membrane ; Multi-pass membrane protein
Features
Showing features for topological domain, transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Topological domain | 1-6 | Cytoplasmic | ||||
Sequence: MKSNNA | ||||||
Transmembrane | 7-27 | Helical | ||||
Sequence: LIVILGTVTLDAVGIGLVMPV | ||||||
Topological domain | 28-45 | Periplasmic | ||||
Sequence: LPGLLRDIVHSDSIASHY | ||||||
Transmembrane | 46-66 | Helical | ||||
Sequence: GVLLALYALMQFLCAPVLGAL | ||||||
Topological domain | 67-79 | Cytoplasmic | ||||
Sequence: SDRFGRRPVLLAS | ||||||
Transmembrane | 80-100 | Helical | ||||
Sequence: LLGATIDYAIMATTPVLWILY | ||||||
Topological domain | 101-103 | Periplasmic | ||||
Sequence: AGR | ||||||
Transmembrane | 104-124 | Helical | ||||
Sequence: IVAGITGATGAVAGAYIADIT | ||||||
Topological domain | 125-138 | Cytoplasmic | ||||
Sequence: DGEDRARHFGLMSA | ||||||
Transmembrane | 139-159 | Helical | ||||
Sequence: CFGVGMVAGPVAGGLLGAISL | ||||||
Topological domain | 160 | Periplasmic | ||||
Sequence: H | ||||||
Transmembrane | 161-181 | Helical | ||||
Sequence: APFLAAAVLNGLNLLLGCFLM | ||||||
Topological domain | 182-210 | Cytoplasmic | ||||
Sequence: QESHKGERRPMPLRAFNPVSSFRWARGMT | ||||||
Transmembrane | 211-231 | Helical | ||||
Sequence: IVAALMTVFFIMQLVGQVPAA | ||||||
Topological domain | 232-246 | Periplasmic | ||||
Sequence: LWVIFGEDRFRWSAT | ||||||
Transmembrane | 247-267 | Helical | ||||
Sequence: MIGLSLAVFGILHALAQAFVT | ||||||
Topological domain | 268-277 | Cytoplasmic | ||||
Sequence: GPATKRFGEK | ||||||
Transmembrane | 278-298 | Helical | ||||
Sequence: QAIIAGMAADALGYVLLAFAT | ||||||
Topological domain | 299 | Periplasmic | ||||
Sequence: R | ||||||
Transmembrane | 300-320 | Helical | ||||
Sequence: GWMAFPIMILLASGGIGMPAL | ||||||
Topological domain | 321-339 | Cytoplasmic | ||||
Sequence: QAMLSRQVDDDHQGQLQGS | ||||||
Transmembrane | 340-360 | Helical | ||||
Sequence: LAALTSLTSITGPLIVTAIYA | ||||||
Topological domain | 361-364 | Periplasmic | ||||
Sequence: ASAS | ||||||
Transmembrane | 365-385 | Helical | ||||
Sequence: TWNGLAWIVGAALYLVCLPAL | ||||||
Topological domain | 386-396 | Cytoplasmic | ||||
Sequence: RRGAWSRATST |
Keywords
- Cellular component
Phenotypes & Variants
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000173394 | 1-396 | Tetracycline resistance protein, class C | |||
Sequence: MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHYGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST |
Structure
Sequence
- Sequence statusComplete
- Length396
- Mass (Da)41,510
- Last updated1986-07-21 v1
- Checksum0B9C82E811A2F7DF
Keywords
- Technical term