P01535 · STX3_ANESU
- ProteinDelta-actitoxin-Avd2a
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Specific arthropod (crab and insect) toxin that inhibits inactivation of voltage-gated sodium channels. It competes well with the site-3 toxin LqhalphaIT (from the scorpion L.quinquestriatus (AC P17728)) on binding to cockroach neuronal membranes (Ki=21.4 nM), and inhibits the inactivation of D.melanogaster channel (DmNav1), but not that of mammalian Navs expressed in Xenopus oocytes. Its activity is synergically enhanced by ligands of receptor site-4 (Bj-xtrIT (AC P56637)). Its ability to inhibit the channel mutant DmNav1[D1701R] only decreases 5-fold, whereas the inhibition activity is completely lost by LqhalphaIT and Av2 when tested on DmNav1[D1701R].
Miscellaneous
Negative results: is inactive on mammals, when tested by subcutaneous injection into mice at a high dose (up to 5940 nmol/kg of mouse). Does not inhibit rNav1.2/SCN2A, rNav1.4/SCN4A, hNav1.5/SCN5A and rNav1.6/SCN8A.
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | extracellular region | |
Cellular Component | nematocyst | |
Molecular Function | sodium channel inhibitor activity | |
Molecular Function | toxin activity |
Keywords
- Molecular function
Names & Taxonomy
Protein names
- Recommended nameDelta-actitoxin-Avd2a
- Short namesDelta-AITX-Avd2a
- Alternative names
Organism names
- Taxonomic lineageEukaryota > Metazoa > Cnidaria > Anthozoa > Hexacorallia > Actiniaria > Actiniidae > Anemonia
Accessions
- Primary accessionP01535
Subcellular Location
Phenotypes & Variants
Toxic dose
PD50 is 1.15 pmol/100 mg (native toxin) or PD50 is 2.65 pmol/100 mg (recombinant toxin) when injected inter-segmentally into blowfly larvae (S.falculata).
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 1 | Low decrease in binding affinity to cockroach neuronal membranes and in toxicity to blowfly larvae. | ||||
Sequence: R → A | ||||||
Mutagenesis | 2 | No change in binding affinity to cockroach neuronal membranes and in toxicity to blowfly larvae. | ||||
Sequence: S → A | ||||||
Mutagenesis | 5 | Low decrease in binding affinity to cockroach neuronal membranes and in toxicity to blowfly larvae. | ||||
Sequence: P → A | ||||||
Mutagenesis | 7 | Loss of binding affinity to cockroach neuronal membranes and of toxicity to blowfly larvae. | ||||
Sequence: Y → A | ||||||
Mutagenesis | 8 | Loss of binding affinity to cockroach neuronal membranes and of toxicity to blowfly larvae. | ||||
Sequence: W → A | ||||||
Mutagenesis | 12 | Loss of binding affinity to cockroach neuronal membranes and of toxicity to blowfly larvae. | ||||
Sequence: P → A | ||||||
Mutagenesis | 13 | Loss of binding affinity to cockroach neuronal membranes and of toxicity to blowfly larvae. | ||||
Sequence: W → A | ||||||
Mutagenesis | 15 | No change in binding affinity to cockroach neuronal membranes and in toxicity to blowfly larvae. | ||||
Sequence: Q → A | ||||||
Mutagenesis | 16 | No change in binding affinity to cockroach neuronal membranes and in toxicity to blowfly larvae. | ||||
Sequence: N → A | ||||||
Mutagenesis | 18 | Loss of binding affinity to cockroach neuronal membranes and of toxicity to blowfly larvae. | ||||
Sequence: Y → A | ||||||
Mutagenesis | 19 | Low decrease in binding affinity to cockroach neuronal membranes and in toxicity to blowfly larvae. | ||||
Sequence: P → A | ||||||
Mutagenesis | 20 | Low increase in binding affinity to cockroach neuronal membranes and in toxicity to blowfly larvae. | ||||
Sequence: E → A | ||||||
Mutagenesis | 23 | Low increase in binding affinity to cockroach neuronal membranes and in toxicity to blowfly larvae. | ||||
Sequence: S → A | ||||||
Mutagenesis | 25 | Low increase in binding affinity to cockroach neuronal membranes and in toxicity to blowfly larvae. | ||||
Sequence: P → A | ||||||
Mutagenesis | 26 | No change in binding affinity to cockroach neuronal membranes and in toxicity to blowfly larvae. | ||||
Sequence: K → A | ||||||
Mutagenesis | 27 | No change in binding affinity to cockroach neuronal membranes and in toxicity to blowfly larvae. | ||||
Sequence: V → A |
PTM/Processing
Features
Showing features for peptide, disulfide bond.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Peptide | PRO_0000044860 | 1-27 | Delta-actitoxin-Avd2a | |||
Sequence: RSCCPCYWGGCPWGQNCYPEGCSGPKV | ||||||
Disulfide bond | 3↔17 | |||||
Sequence: CCPCYWGGCPWGQNC | ||||||
Disulfide bond | 4↔11 | |||||
Sequence: CPCYWGGC | ||||||
Disulfide bond | 6↔22 | |||||
Sequence: CYWGGCPWGQNCYPEGC |
Keywords
- PTM
Structure
Sequence
- Sequence statusComplete
- Length27
- Mass (Da)2,938
- Last updated1986-07-21 v1
- ChecksumAA4E261FFAF34A7A
Features
Showing features for sequence conflict.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Sequence conflict | 22-23 | in Ref. 2; AA sequence | ||||
Sequence: CS → SC |
Keywords
- Technical term