P00942 · TPIS_YEAST
- ProteinTriosephosphate isomerase
- GeneTPI1
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids248 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
Miscellaneous
Present with 207000 molecules/cell in log phase SD medium.
Catalytic activity
- D-glyceraldehyde 3-phosphate = dihydroxyacetone phosphate
Pathway
Carbohydrate biosynthesis; gluconeogenesis.
Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate from glycerone phosphate: step 1/1.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 10 | substrate | ||||
Sequence: N | ||||||
Binding site | 12 | substrate | ||||
Sequence: K | ||||||
Active site | 95 | Electrophile | ||||
Sequence: H | ||||||
Active site | 165 | Proton acceptor | ||||
Sequence: E |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Cellular Component | cytosol | |
Cellular Component | mitochondrion | |
Cellular Component | plasma membrane | |
Molecular Function | triose-phosphate isomerase activity | |
Biological Process | gluconeogenesis | |
Biological Process | glyceraldehyde-3-phosphate biosynthetic process | |
Biological Process | glycerol catabolic process | |
Biological Process | glycolytic process |
Keywords
- Molecular function
- Biological process
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameTriosephosphate isomerase
- EC number
- Short namesTIM
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Fungi > Dikarya > Ascomycota > Saccharomycotina > Saccharomycetes > Saccharomycetales > Saccharomycetaceae > Saccharomyces
Accessions
- Primary accessionP00942
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
PTM/Processing
Features
Showing features for initiator methionine, chain, modified residue, cross-link.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Initiator methionine | 1 | Removed | ||||
Sequence: M | ||||||
Chain | PRO_0000090169 | 2-248 | Triosephosphate isomerase | |||
Sequence: ARTFFVGGNFKLNGSKQSIKEIVERLNTASIPENVEVVICPPATYLDYSVSLVKKPQVTVGAQNAYLKASGAFTGENSVDQIKDVGAKWVILGHSERRSYFHEDDKFIADKTKFALGQGVGVILCIGETLEEKKAGKTLDVVERQLNAVLEEVKDWTNVVVAYEPVWAIGTGLAATPEDAQDIHASIRKFLASKLGDKAASELRILYGGSANGSNAVTFKDKADVDGFLVGGASLKPEFVDIINSRN | ||||||
Modified residue | 4 | Phosphothreonine | ||||
Sequence: T | ||||||
Modified residue | 71 | Phosphoserine | ||||
Sequence: S | ||||||
Modified residue | 215 | Phosphoserine | ||||
Sequence: S | ||||||
Cross-link | 223 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) | ||||
Sequence: K |
Keywords
- PTM
Proteomic databases
PTM databases
Structure
Sequence
- Sequence statusComplete
- Length248
- Mass (Da)26,795
- Last updated2007-01-23 v2
- Checksum8429251C4DF51F86
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
J01366 EMBL· GenBank· DDBJ | AAA88757.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
Z49209 EMBL· GenBank· DDBJ | CAA89080.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AY557654 EMBL· GenBank· DDBJ | AAS55980.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BK006938 EMBL· GenBank· DDBJ | DAA11897.1 EMBL· GenBank· DDBJ | Genomic DNA |