P00905 · TRPGD_SALTY

Function

function

Part of a heterotetrameric complex that catalyzes the two-step biosynthesis of anthranilate, an intermediate in the biosynthesis of L-tryptophan. In the first step, the glutamine-binding beta subunit (TrpG) of anthranilate synthase (AS) provides the glutamine amidotransferase activity which generates ammonia as a substrate that, along with chorismate, is used in the second step, catalyzed by the large alpha subunit of AS (TrpE) to produce anthranilate. In the absence of TrpG, TrpE can synthesize anthranilate directly from chorismate and high concentrations of ammonia. In addition to synthesizing anthranilate, it also catalyzes the second step of the pathway, the transfer of the phosphoribosyl group of 5-phosphorylribose-1-pyrophosphate (PRPP) to anthranilate.

Catalytic activity

Activity regulation

Cooperatively feedback inhibited by tryptophan.

Pathway

Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 1/5.
Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 2/5.

Features

Showing features for binding site, active site.

TypeIDPosition(s)Description
Binding site57-59L-glutamine (UniProtKB | ChEBI)
Active site84Nucleophile; for GATase activity
Binding site88L-glutamine (UniProtKB | ChEBI)
Binding site134-135L-glutamine (UniProtKB | ChEBI)
Active site170For GATase activity
Active site172For GATase activity

GO annotations

AspectTerm
Molecular Functionanthranilate phosphoribosyltransferase activity
Molecular Functionanthranilate synthase activity
Molecular Functionmagnesium ion binding
Biological Processglutamine metabolic process
Biological Processphenazine biosynthetic process
Biological Processtryptophan biosynthetic process

Keywords

Enzyme and pathway databases

Protein family/group databases

Names & Taxonomy

Protein names

  • Recommended name
    Bifunctional protein TrpGD

Including 2 domains:

  • Recommended name
    Anthranilate synthase component 2
  • EC number
  • Short names
    AS; ASII
  • Alternative names
    • Anthranilate synthase, glutamine amidotransferase component
  • Recommended name
    Anthranilate phosphoribosyltransferase
  • EC number

Gene names

    • Name
      trpGD
    • Synonyms
      trpD
    • Ordered locus names
      STM1724

Organism names

Accessions

  • Primary accession
    P00905

Proteomes

PTM/Processing

Features

Showing features for initiator methionine, chain.

TypeIDPosition(s)Description
Initiator methionine1Removed
ChainPRO_00000568972-531Bifunctional protein TrpGD

Proteomic databases

Interaction

Subunit

Monomer. Heterotetramer consisting of two non-identical subunits: a beta subunit (TrpG) and a large alpha subunit (TrpE) (Probable).

Protein-protein interaction databases

Family & Domains

Features

Showing features for domain, region.

TypeIDPosition(s)Description
Domain3-196Glutamine amidotransferase type-1
Region202-531Anthranilate phosphoribosyltransferase

Sequence similarities

In the C-terminal section; belongs to the anthranilate phosphoribosyltransferase family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    531
  • Mass (Da)
    56,918
  • Last updated
    2007-01-23 v4
  • Checksum
    256D2409062CD4C7
MADILLLDNIDSFTWNLADQLRTNGHNVVIYRNHIPAQTLIDRLATMKNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAGEILHGKASSIEHDGQAMFAGLANPLPVARYHSLVGSNVPAGLTINAHFNGMVMAVRHDADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPTNTLQPILEKLYQAQTLTQQESHQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPEYLFADIVGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNADKSRQALDELGVCFLFAPKYHTGLRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGVYSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGEIKSYQLTAEDFGLTPYHQDQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGQEDLKANAQTVLDVLRNGTAYDRVTALAARG

Features

Showing features for sequence conflict.

TypeIDPosition(s)Description
Sequence conflict191-192in Ref. 3; AAA57312
Sequence conflict511in Ref. 1; AAA27236

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AH000942
EMBL· GenBank· DDBJ
AAA27236.1
EMBL· GenBank· DDBJ
Genomic DNA
AE006468
EMBL· GenBank· DDBJ
AAL20642.1
EMBL· GenBank· DDBJ
Genomic DNA
J01811
EMBL· GenBank· DDBJ
AAA57312.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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