P00888 · AROF_ECOLI

  • Protein
    Phospho-2-dehydro-3-deoxyheptonate aldolase, Tyr-sensitive
  • Gene
    aroF
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at protein level
  • Annotation score
    4/5

Function

function

Stereospecific condensation of phosphoenolpyruvate (PEP) and D-erythrose-4-phosphate (E4P) giving rise to 3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP).

Miscellaneous

There are 3 DAHP synthases, AroF is feedback-inhibited by Tyr. The other 2 DAHP synthases are Phe- and Trp-sensitive, respectively.

Catalytic activity

Cofactor

a divalent metal cation (UniProtKB | Rhea| CHEBI:60240 )

Activity regulation

Specifically feedback inhibited by tyrosine with 50% inhibition observed at 9 microM tyrosine, pH 7.0.

Pathway

Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 1/7.

GO annotations

AspectTerm
Cellular Componentcytoplasm
Molecular Function3-deoxy-7-phosphoheptulonate synthase activity
Molecular Functionidentical protein binding
Biological Processamino acid biosynthetic process
Biological Processaromatic amino acid family biosynthetic process
Biological Processchorismate biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Phospho-2-dehydro-3-deoxyheptonate aldolase, Tyr-sensitive
  • EC number
  • Alternative names
    • 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase
    • DAHP synthase
    • Phospho-2-keto-3-deoxyheptonate aldolase

Gene names

    • Name
      aroF
    • Ordered locus names
      b2601, JW2582

Organism names

  • Taxonomic identifier
  • Strains
    • K12 / W3110 / ATCC 27325 / DSM 5911
    • K12 / MG1655 / ATCC 47076
  • Taxonomic lineage
    Bacteria > Pseudomonadota > Gammaproteobacteria > Enterobacterales > Enterobacteriaceae > Escherichia

Accessions

  • Primary accession
    P00888
  • Secondary accessions
    • Q47061

Proteomes

Subcellular Location

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00001408311-356Phospho-2-dehydro-3-deoxyheptonate aldolase, Tyr-sensitive

Proteomic databases

Interaction

Protein-protein interaction databases

Family & Domains

Sequence similarities

Belongs to the class-I DAHP synthase family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    356
  • Mass (Da)
    38,804
  • Last updated
    1987-08-13 v1
  • Checksum
    A675537531FBC37D
MQKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKSISDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTTVGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSWSAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHRFVGINQAGQVALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDYRRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWEMTDALLREIHQDLNGQLTARVA

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
K01989
EMBL· GenBank· DDBJ
AAA23489.1
EMBL· GenBank· DDBJ
Genomic DNA
M10431
EMBL· GenBank· DDBJ
AAA24332.1
EMBL· GenBank· DDBJ
Genomic DNA
U00096
EMBL· GenBank· DDBJ
AAC75650.1
EMBL· GenBank· DDBJ
Genomic DNA
AP009048
EMBL· GenBank· DDBJ
BAA16487.1
EMBL· GenBank· DDBJ
Genomic DNA
M60890
EMBL· GenBank· DDBJ
AAA23491.1
EMBL· GenBank· DDBJ
Genomic DNA
K03453
EMBL· GenBank· DDBJ
AAA23490.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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