P00888 · AROF_ECOLI
- ProteinPhospho-2-dehydro-3-deoxyheptonate aldolase, Tyr-sensitive
- GenearoF
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids356 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
Stereospecific condensation of phosphoenolpyruvate (PEP) and D-erythrose-4-phosphate (E4P) giving rise to 3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP).
Miscellaneous
There are 3 DAHP synthases, AroF is feedback-inhibited by Tyr. The other 2 DAHP synthases are Phe- and Trp-sensitive, respectively.
Catalytic activity
- D-erythrose 4-phosphate + phosphoenolpyruvate + H2O = 7-phospho-2-dehydro-3-deoxy-D-arabino-heptonate + phosphate
Cofactor
Activity regulation
Specifically feedback inhibited by tyrosine with 50% inhibition observed at 9 microM tyrosine, pH 7.0.
Pathway
Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 1/7.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | 3-deoxy-7-phosphoheptulonate synthase activity | |
Molecular Function | identical protein binding | |
Biological Process | amino acid biosynthetic process | |
Biological Process | aromatic amino acid family biosynthetic process | |
Biological Process | chorismate biosynthetic process |
Keywords
- Molecular function
- Biological process
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended namePhospho-2-dehydro-3-deoxyheptonate aldolase, Tyr-sensitive
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strains
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Enterobacterales > Enterobacteriaceae > Escherichia
Accessions
- Primary accessionP00888
- Secondary accessions
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Structure
Sequence
- Sequence statusComplete
- Length356
- Mass (Da)38,804
- Last updated1987-08-13 v1
- ChecksumA675537531FBC37D
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
K01989 EMBL· GenBank· DDBJ | AAA23489.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
M10431 EMBL· GenBank· DDBJ | AAA24332.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
U00096 EMBL· GenBank· DDBJ | AAC75650.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AP009048 EMBL· GenBank· DDBJ | BAA16487.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
M60890 EMBL· GenBank· DDBJ | AAA23491.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
K03453 EMBL· GenBank· DDBJ | AAA23490.1 EMBL· GenBank· DDBJ | Genomic DNA |