P00609 · PA2B5_NOTSC
- ProteinBasic phospholipase A2 notechis II-5
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids119 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
Snake venom phospholipase A2 (PLA2) that inhibits neuromuscular transmission by blocking acetylcholine release from the nerve termini. Notechis II-5 is less toxic than notexin but has a higher specific phospholipase activity. PLA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides.
Catalytic activity
- a 1,2-diacyl-sn-glycero-3-phosphocholine + H2O = a 1-acyl-sn-glycero-3-phosphocholine + a fatty acid + H+
Cofactor
Note: Binds 1 Ca2+ ion.
Features
Showing features for binding site, active site.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | extracellular region | |
Molecular Function | calcium ion binding | |
Molecular Function | calcium-dependent phospholipase A2 activity | |
Molecular Function | phospholipid binding | |
Molecular Function | toxin activity | |
Biological Process | arachidonic acid secretion | |
Biological Process | lipid catabolic process | |
Biological Process | phospholipid metabolic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameBasic phospholipase A2 notechis II-5
- EC number
- Short namessvPLA2
- Alternative names
Organism names
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Lepidosauria > Squamata > Bifurcata > Unidentata > Episquamata > Toxicofera > Serpentes > Colubroidea > Elapidae > Acanthophiinae > Notechis
Accessions
- Primary accessionP00609
Subcellular Location
Phenotypes & Variants
Toxic dose
LD50 is 0.045 mg/kg by intravenous injection.
PTM/Processing
Features
Showing features for chain, disulfide bond.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000161676 | 1-119 | Basic phospholipase A2 notechis II-5 | |||
Sequence: NLVQFSYLIQCANHGRRPTRHYMDYGCYCGWGGSGTPVDELDRCCKIHDDCYSDAEKKGCSPKMSAYDYYCGENGPYCRNIKKKCLRFVCDCDVEAAFCFAKAPYNNANWNIDTKKRCQ | ||||||
Disulfide bond | 11↔71 | |||||
Sequence: CANHGRRPTRHYMDYGCYCGWGGSGTPVDELDRCCKIHDDCYSDAEKKGCSPKMSAYDYYC | ||||||
Disulfide bond | 27↔118 | |||||
Sequence: CYCGWGGSGTPVDELDRCCKIHDDCYSDAEKKGCSPKMSAYDYYCGENGPYCRNIKKKCLRFVCDCDVEAAFCFAKAPYNNANWNIDTKKRC | ||||||
Disulfide bond | 29↔45 | |||||
Sequence: CGWGGSGTPVDELDRCC | ||||||
Disulfide bond | 44↔99 | |||||
Sequence: CCKIHDDCYSDAEKKGCSPKMSAYDYYCGENGPYCRNIKKKCLRFVCDCDVEAAFC | ||||||
Disulfide bond | 51↔92 | |||||
Sequence: CYSDAEKKGCSPKMSAYDYYCGENGPYCRNIKKKCLRFVCDC | ||||||
Disulfide bond | 60↔85 | |||||
Sequence: CSPKMSAYDYYCGENGPYCRNIKKKC | ||||||
Disulfide bond | 78↔90 | |||||
Sequence: CRNIKKKCLRFVC |
Keywords
- PTM
Expression
Tissue specificity
Expressed by the venom gland.
Structure
Family & Domains
Sequence similarities
Family and domain databases
Sequence
- Sequence statusComplete
- Length119
- Mass (Da)13,676
- Last updated1986-07-21 v1
- Checksum032C9EEBF62BEE26
Keywords
- Technical term