P00565 · KCRM_CHICK
- ProteinCreatine kinase M-type
- GeneCKM
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids381 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate) (PubMed:8525081).
Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa (Probable)
Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa (Probable)
Catalytic activity
- ATP + creatine = ADP + H+ + N-phosphocreatineThis reaction proceeds in the forward direction.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 128-132 | ATP (UniProtKB | ChEBI) | ||||
Sequence: SSRVR | ||||||
Binding site | 191 | ATP (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Binding site | 236 | ATP (UniProtKB | ChEBI) | ||||
Sequence: R | ||||||
Binding site | 292 | ATP (UniProtKB | ChEBI) | ||||
Sequence: R | ||||||
Binding site | 320-325 | ATP (UniProtKB | ChEBI) | ||||
Sequence: RGTGGV | ||||||
Binding site | 335 | ATP (UniProtKB | ChEBI) | ||||
Sequence: D |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Cellular Component | extracellular space | |
Molecular Function | ATP binding | |
Molecular Function | creatine kinase activity | |
Biological Process | phosphocreatine biosynthetic process | |
Biological Process | response to heat |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameCreatine kinase M-type
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Archelosauria > Archosauria > Dinosauria > Saurischia > Theropoda > Coelurosauria > Aves > Neognathae > Galloanserae > Galliformes > Phasianidae > Phasianinae > Gallus
Accessions
- Primary accessionP00565
Proteomes
Organism-specific databases
Subcellular Location
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000211980 | 1-381 | Creatine kinase M-type | |||
Sequence: MPFSSTHNKHKLKFSAEEEFPDLSKHNNHMAKVLTPELYKRLRDKETPSGFTLDDVIQTGVDNPGHPFIMTVGCVAGDEESYEVFKDLFDPVIQDRHGGYKPTDKHRTDLNHENLKGGDDLDPKYVLSSRVRTGRSIKGYSLPPHCSRGERRAVEKLSVEALNSLEGEFKGRYYPLKAMTEQEQQQLIDDHFLFDKPVSPLLLASGMARDWPDARGIWHNDNKTFLVWVNEEDHLRVISMEKGGNMKEVFRRFCVGLKKIEEIFKKAGHPFMWTEHLGYILTCPSNLGTGLRGGVHVKLPKLSQHPKFEEILHRLRLQKRGTGGVDTAAVGAVFDISNADRLGFSEVEQVQMVVDGVKLMVEMEKKLEQNQPIDDMIPAQK |
PTM databases
Expression
Tissue specificity
Predominantly found in skeletal muscle, but not in the heart.
Interaction
Subunit
Dimer of identical or non-identical chains, which can be either B (brain type) or M (muscle type). With MM being the major form in skeletal muscle and myocardium, MB existing in myocardium, and BB existing in many tissues, especially brain.
Structure
Family & Domains
Features
Showing features for domain, region.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 11-98 | Phosphagen kinase N-terminal | ||||
Sequence: KLKFSAEEEFPDLSKHNNHMAKVLTPELYKRLRDKETPSGFTLDDVIQTGVDNPGHPFIMTVGCVAGDEESYEVFKDLFDPVIQDRHG | ||||||
Region | 99-118 | Disordered | ||||
Sequence: GYKPTDKHRTDLNHENLKGG | ||||||
Domain | 125-367 | Phosphagen kinase C-terminal | ||||
Sequence: YVLSSRVRTGRSIKGYSLPPHCSRGERRAVEKLSVEALNSLEGEFKGRYYPLKAMTEQEQQQLIDDHFLFDKPVSPLLLASGMARDWPDARGIWHNDNKTFLVWVNEEDHLRVISMEKGGNMKEVFRRFCVGLKKIEEIFKKAGHPFMWTEHLGYILTCPSNLGTGLRGGVHVKLPKLSQHPKFEEILHRLRLQKRGTGGVDTAAVGAVFDISNADRLGFSEVEQVQMVVDGVKLMVEMEKKL |
Sequence similarities
Belongs to the ATP:guanido phosphotransferase family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length381
- Mass (Da)43,328
- Last updated1986-07-21 v1
- Checksum192C236BB46E2531
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
M10012 EMBL· GenBank· DDBJ | AAA48689.1 EMBL· GenBank· DDBJ | mRNA | ||
X00954 EMBL· GenBank· DDBJ | CAA25465.1 EMBL· GenBank· DDBJ | mRNA | ||
X00954 EMBL· GenBank· DDBJ | CAA25466.2 EMBL· GenBank· DDBJ | mRNA |