P00507 · AATM_RAT

  • Protein
    Aspartate aminotransferase, mitochondrial
  • Gene
    Got2
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at protein level
  • Annotation score
    5/5

Function

function

Catalyzes the irreversible transamination of the L-tryptophan metabolite L-kynurenine to form kynurenic acid (KA). As a member of the malate-aspartate shuttle, it has a key role in the intracellular NAD(H) redox balance. Is important for metabolite exchange between mitochondria and cytosol, and for amino acid metabolism. Facilitates cellular uptake of long-chain free fatty acids (By similarity).

Miscellaneous

In eukaryotes there are cytoplasmic, mitochondrial and chloroplastic isozymes.

Catalytic activity

Cofactor

pyridoxal 5'-phosphate (UniProtKB | Rhea| CHEBI:597326 )

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site65substrate
Binding site162substrate
Binding site215substrate
Binding site407substrate

GO annotations

AspectTerm
Cellular Componentcell surface
Cellular Componentmitochondrial inner membrane
Cellular Componentmitochondrial matrix
Cellular Componentmitochondrion
Cellular Componentperikaryon
Cellular Componentplasma membrane
Cellular Componentprotein-containing complex
Cellular Componentsarcolemma
Cellular ComponentT-tubule
Molecular Functionamino acid binding
Molecular Functioncarboxylic acid binding
Molecular Functionenzyme binding
Molecular Functionidentical protein binding
Molecular Functionkynurenine-oxoglutarate transaminase activity
Molecular FunctionL-aspartate:2-oxoglutarate aminotransferase activity
Molecular Functionphospholipid binding
Molecular Functionpyridoxal phosphate binding
Biological Process2-oxoglutarate metabolic process
Biological Processamino acid metabolic process
Biological Processaspartate biosynthetic process
Biological Processaspartate catabolic process
Biological Processaspartate metabolic process
Biological Processdicarboxylic acid metabolic process
Biological Processfatty acid transport
Biological Processfemale pregnancy
Biological Processglutamate catabolic process to 2-oxoglutarate
Biological Processglutamate catabolic process to aspartate
Biological Processglutamate metabolic process
Biological Processlactation
Biological Processoxaloacetate metabolic process
Biological Processresponse to ethanol
Biological Processresponse to insulin
Biological Processresponse to muscle activity

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Aspartate aminotransferase, mitochondrial
  • EC number
  • Short names
    mAspAT
  • Alternative names
    • Fatty acid-binding protein (FABP-1)
    • Glutamate oxaloacetate transaminase 2
    • Kynurenine aminotransferase 4
    • Kynurenine aminotransferase IV
    • Kynurenine--oxoglutarate transaminase 4

Gene names

    • Name
      Got2
    • Synonyms
      Maat

Organism names

  • Taxonomic identifier
  • Strains
    • Wistar
    • Sprague-Dawley
  • Taxonomic lineage
    Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Rattus

Accessions

  • Primary accession
    P00507
  • Secondary accessions
    • Q64551
    • Q9QV50

Proteomes

Organism-specific databases

Subcellular Location

Mitochondrion matrix
Cell membrane
Note: Located in the mitochondria of liver, pancreas, spleen, heart, pituitary gland and submandibular gland cells. In kidney, located in the mitochondria, on the cell surface of regions with protrusions in distal tubules, on the apical cell surface of protrusions along the microvilli in cortical collecting ducts, in condensing vacuoles, on the cell surface at cell boundaries of adjoining kidney cells and on the cell surface of endothelial cells lining capillaries in the glomerulus. Also located at the cell surface of endothelial cells lining arterioles and on the cell surface of lymphocytes.

Keywords

Phenotypes & Variants

PTM/Processing

Features

Showing features for transit peptide, chain, modified residue.

TypeIDPosition(s)Description
Transit peptide1-29Mitochondrion
ChainPRO_000000121830-430Aspartate aminotransferase, mitochondrial
Modified residue48Phosphothreonine
Modified residue59N6-acetyllysine
Modified residue73N6-acetyllysine; alternate
Modified residue73N6-succinyllysine; alternate
Modified residue82N6-acetyllysine
Modified residue90N6-acetyllysine; alternate
Modified residue90N6-succinyllysine; alternate
Modified residue963'-nitrotyrosine; alternate
Modified residue96Phosphotyrosine; alternate
Modified residue107N6-acetyllysine; alternate
Modified residue107N6-succinyllysine; alternate
Modified residue122N6-acetyllysine; alternate
Modified residue122N6-succinyllysine; alternate
Modified residue143Phosphoserine
Modified residue159N6-acetyllysine; alternate
Modified residue159N6-succinyllysine; alternate
Modified residue185N6-acetyllysine; alternate
Modified residue185N6-succinyllysine; alternate
Modified residue227N6-succinyllysine
Modified residue234N6-acetyllysine
Modified residue279N6-(pyridoxal phosphate)lysine; alternate
Modified residue279N6-acetyllysine; alternate
Modified residue296N6-acetyllysine; alternate
Modified residue296N6-succinyllysine; alternate
Modified residue302N6-acetyllysine
Modified residue309N6-acetyllysine; alternate
Modified residue309N6-succinyllysine; alternate
Modified residue313Asymmetric dimethylarginine
Modified residue338N6-acetyllysine; alternate
Modified residue338N6-succinyllysine; alternate
Modified residue345N6-acetyllysine
Modified residue363N6-acetyllysine; alternate
Modified residue363N6-succinyllysine; alternate
Modified residue364N6-acetyllysine
Modified residue387N6-acetyllysine
Modified residue396N6-acetyllysine; alternate
Modified residue396N6-succinyllysine; alternate
Modified residue404N6-acetyllysine; alternate
Modified residue404N6-succinyllysine; alternate

Keywords

Proteomic databases

PTM databases

Expression

Tissue specificity

Expressed in all tissues tested: liver, pancreas, kidney, heart, spleen, arterioles, and lymphocytes.

Gene expression databases

Interaction

Subunit

Homodimer.

Protein-protein interaction databases

Structure

Family & Domains

Sequence similarities

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Sequence processing
    The displayed sequence is further processed into a mature form.
  • Length
    430
  • Mass (Da)
    47,314
  • Last updated
    1989-07-01 v2
  • Checksum
    EDC8B862A20DB736
MALLHSGRVLSGMAAAFHPGLAAAASARASSWWTHVEMGPPDPILGVTEAFKRDTNSKKMNLGVGAYRDDNGKPYVLPSVRKAEAQIAGKNLDKEYLPIGGLADFCKASAELALGENSEVLKSGRFVTVQTISGTGALRVGASFLQRFFKFSRDVFLPKPSWGNHTPIFRDAGMQLQGYRYYDPKTCGFDFSGALEDISKIPEQSVLLLHACAHNPTGVDPRPEQWKEMAAVVKKKNLFAFFDMAYQGFASGDGDKDAWAVRHFIEQGINVCLCQSYAKNMGLYGERVGAFTVVCKDAEEAKRVESQLKILIRPLYSNPPLNGARIAATILTSPDLRKQWLQEVKGMADRIISMRTQLVSNLKKEGSSHNWQHITDQIGMFCFTGLKPEQVERLTKEFSVYMTKDGRISVAGVTSGNVGYLAHAIHQVTK

Computationally mapped potential isoform sequences

There are 2 potential isoforms mapped to this entry

View all
EntryEntry nameGene nameLength
A0A8L2Q7Q0A0A8L2Q7Q0_RATGot2426
A0A8I6GLH0A0A8I6GLH0_RATGot2410

Features

Showing features for sequence conflict.

TypeIDPosition(s)Description
Sequence conflict30in Ref. 3; AAC13868
Sequence conflict162in Ref. 4; AA sequence and 5; AA sequence
Sequence conflict167-169in Ref. 4; AA sequence and 5; AA sequence
Sequence conflict177in Ref. 4; AA sequence and 5; AA sequence
Sequence conflict232in Ref. 4; AA sequence and 5; AA sequence
Sequence conflict255in Ref. 4; AA sequence and 5; AA sequence
Sequence conflict338-339in Ref. 4; AA sequence and 5; AA sequence
Sequence conflict352in Ref. 4; AA sequence and 5; AA sequence
Sequence conflict386in Ref. 4; AA sequence and 5; AA sequence
Sequence conflict400in Ref. 4; AA sequence and 5; AA sequence

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
M18467
EMBL· GenBank· DDBJ
AAB54275.1
EMBL· GenBank· DDBJ
mRNA
BC061792
EMBL· GenBank· DDBJ
AAH61792.1
EMBL· GenBank· DDBJ
mRNA
U21158
EMBL· GenBank· DDBJ
AAC13868.1
EMBL· GenBank· DDBJ
Genomic DNA
M12709
EMBL· GenBank· DDBJ
AAA41267.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

Disclaimer

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