P00459 · NIFH1_AZOVI
- ProteinNitrogenase iron protein 1
- GenenifH1
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids290 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
The key enzymatic reactions in nitrogen fixation are catalyzed by the nitrogenase complex, which has 2 components: the iron protein (component 2) and a component 1 which is either a molybdenum-iron protein, a vanadium-iron, or an iron-iron protein.
Miscellaneous
This subunit is associated with the molybdenum-iron nitrogenase component 2.
Catalytic activity
- 16 ATP + 16 H2O + N2 + 8 reduced [2Fe-2S]-[ferredoxin] = 16 ADP + 6 H+ + H2 + 2 NH4+ + 8 oxidized [2Fe-2S]-[ferredoxin] + 16 phosphate
16 CHEBI:30616 + 16 CHEBI:15377 + CHEBI:17997 + 8 RHEA-COMP:10001 = 16 CHEBI:456216 + 6 CHEBI:15378 + CHEBI:18276 + 2 CHEBI:28938 + 8 RHEA-COMP:10000 + 16 CHEBI:43474
Cofactor
Note: Binds 1 [4Fe-4S] cluster per dimer.
Activity regulation
Nitrogenase holoenzyme is subject to 'conformational protection' by FeSII; under oxidizing conditions FeSII binds to the holoenzyme and reversibly protects it from oxidation (PubMed:7830548).
Features
Showing features for binding site.
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | 4 iron, 4 sulfur cluster binding | |
Molecular Function | ATP binding | |
Molecular Function | carbonyl sulfide nitrogenase activity | |
Molecular Function | metal ion binding | |
Molecular Function | nitrogenase activity | |
Biological Process | nitrogen fixation |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameNitrogenase iron protein 1
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Pseudomonadales > Pseudomonadaceae > Azotobacter
Accessions
- Primary accessionP00459
Phenotypes & Variants
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 16 | Loss of nitrogen fixation. | ||||
Sequence: K → Q or P |
PTM/Processing
Features
Showing features for initiator methionine, chain, modified residue.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Initiator methionine | 1 | Removed | ||||
Sequence: M | ||||||
Chain | PRO_0000139490 | 2-290 | Nitrogenase iron protein 1 | |||
Sequence: AMRQCAIYGKGGIGKSTTTQNLVAALAEMGKKVMIVGCDPKADSTRLILHSKAQNTIMEMAAEAGTVEDLELEDVLKAGYGGVKCVESGGPEPGVGCAGRGVITAINFLEEEGAYEDDLDFVFYDVLGDVVCGGFAMPIRENKAQEIYIVCSGEMMAMYAANNISKGIVKYANSGSVRLGGLICNSRNTDREDELIIALANKLGTQMIHFVPRDNVVQRAEIRRMTVIEYDPKAKQADEYRALARKVVDNKLLVIPNPITMDELEELLMEFGIMEVEDESIVGKTAEEV | ||||||
Modified residue | 101 | ADP-ribosylarginine; by dinitrogenase reductase ADP-ribosyltransferase | ||||
Sequence: R |
Post-translational modification
The reversible ADP-ribosylation of Arg-101 inactivates the nitrogenase reductase and regulates nitrogenase activity.
Keywords
- PTM
Expression
Induction
Constitutively expressed during log and stationary phase in sucrose-limited cultures, its levels decrease during stationary phase (at protein level).
Interaction
Subunit
Homodimer.
Structure
Sequence
- Sequence statusComplete
- Length290
- Mass (Da)31,516
- Last updated2007-01-23 v2
- ChecksumC36EFC0D9A27F64D
Features
Showing features for sequence conflict.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Sequence conflict | 145 | in Ref. 2; AAA22142 | ||||
Sequence: A → P |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
M20568 EMBL· GenBank· DDBJ | AAA64709.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
M11579 EMBL· GenBank· DDBJ | AAA22142.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
X06886 EMBL· GenBank· DDBJ | CAA30003.1 EMBL· GenBank· DDBJ | Genomic DNA |