O97581 · LHX3_PIG
- ProteinLIM/homeobox protein Lhx3
- GeneLHX3
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids383 (go to sequence)
- Protein existenceEvidence at transcript level
- Annotation score4/5
Function
function
Transcription factor (PubMed:10195693).
Recognizes and binds to the consensus sequence motif 5'-AATTAATTA-3' in the regulatory elements of target genes, such as glycoprotein hormones alpha chain CGA and visual system homeobox CHX10, positively modulating transcription; transcription can be co-activated by LDB2 (By similarity).
Synergistically enhances transcription from the prolactin promoter in cooperation with POU1F1/Pit-1 (PubMed:10195693).
Required for the establishment of the specialized cells of the pituitary gland and the nervous system (By similarity).
Involved in the development of interneurons and motor neurons in cooperation with LDB1 and ISL1 (By similarity).
Recognizes and binds to the consensus sequence motif 5'-AATTAATTA-3' in the regulatory elements of target genes, such as glycoprotein hormones alpha chain CGA and visual system homeobox CHX10, positively modulating transcription; transcription can be co-activated by LDB2 (By similarity).
Synergistically enhances transcription from the prolactin promoter in cooperation with POU1F1/Pit-1 (PubMed:10195693).
Required for the establishment of the specialized cells of the pituitary gland and the nervous system (By similarity).
Involved in the development of interneurons and motor neurons in cooperation with LDB1 and ISL1 (By similarity).
Features
Showing features for dna binding.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
DNA binding | 142-201 | Homeobox | ||||
Sequence: AKRPRTTITAKQLETLKSAYNTSPKPARHVREQLSSETGLDMRVVQVWFQNRRAKEKRLK |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | nucleus | |
Molecular Function | DNA binding | |
Molecular Function | DNA-binding transcription factor activity, RNA polymerase II-specific | |
Molecular Function | RNA polymerase II transcription regulatory region sequence-specific DNA binding | |
Molecular Function | zinc ion binding | |
Biological Process | neuron differentiation | |
Biological Process | positive regulation of DNA-templated transcription | |
Biological Process | regulation of DNA-templated transcription | |
Biological Process | regulation of transcription by RNA polymerase II |
Keywords
- Molecular function
- Biological process
- Ligand
Names & Taxonomy
Protein names
- Recommended nameLIM/homeobox protein Lhx3
- Short namesLIM homeobox protein 3
- Alternative names
Gene names
Organism names
- Organism
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Laurasiatheria > Artiodactyla > Suina > Suidae > Sus
Accessions
- Primary accessionO97581
Proteomes
Subcellular Location
PTM/Processing
Features
Showing features for chain, modified residue.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000075783 | 1-383 | LIM/homeobox protein Lhx3 | |||
Sequence: WEGRPQELGGKEIPLCAGCDQHILDRFILKALDRHWHSKCLKCSDCHTPLAERCFSRGESLYCKDDFFKRFGTKCAACQLGIPPTQVVRRAQDFVYHLHCFACVVCKRQLATGDEFYLMEDSRLVCKADYETAKQREAEATAKRPRTTITAKQLETLKSAYNTSPKPARHVREQLSSETGLDMRVVQVWFQNRRAKEKRLKKDAGRQRWGQYFRNMKRARGGSKSDKDSVQEEGQDSDAEVSFTDEPSMAEMGPANGLYGGLGEPAPALGRPSGAPGSFPLEHGGLAGPEQYGELRPSSPYGVPSSPAALQSLPGPQPLLSSLVYPEAGLGLVPAGPPGGPPPMRVLAGNGPSSDLSTGSSGGYPDFPASPASWLDEVDHAQF | ||||||
Modified residue | 48 | Phosphothreonine | ||||
Sequence: T | ||||||
Modified residue | 56 | Phosphoserine | ||||
Sequence: S | ||||||
Modified residue | 212 | Phosphotyrosine | ||||
Sequence: Y | ||||||
Modified residue | 223 | Phosphoserine | ||||
Sequence: S |
Keywords
- PTM
Interaction
Subunit
Interacts with POU1F1 (By similarity).
At neuronal promoters, interacts with LDB1, in motor neurons LDB1 is displaced by ISL1 and a ternary complex is formed in which ISL1 contacts both LHX3 and LDB1; allosteric structural changes in the DNA binding domain of LHX3, induced by the ISL1-LHX3 interaction, may explain differences in sequence specificity of the different complexes. Interacts with LDB2. May interact with CITED2/MRG1 (By similarity).
At neuronal promoters, interacts with LDB1, in motor neurons LDB1 is displaced by ISL1 and a ternary complex is formed in which ISL1 contacts both LHX3 and LDB1; allosteric structural changes in the DNA binding domain of LHX3, induced by the ISL1-LHX3 interaction, may explain differences in sequence specificity of the different complexes. Interacts with LDB2. May interact with CITED2/MRG1 (By similarity).
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for domain, region, compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 14-73 | LIM zinc-binding 1 | ||||
Sequence: PLCAGCDQHILDRFILKALDRHWHSKCLKCSDCHTPLAERCFSRGESLYCKDDFFKRFGT | ||||||
Domain | 73-136 | LIM zinc-binding 2 | ||||
Sequence: TKCAACQLGIPPTQVVRRAQDFVYHLHCFACVVCKRQLATGDEFYLMEDSRLVCKADYETAKQR | ||||||
Region | 197-383 | Disordered | ||||
Sequence: EKRLKKDAGRQRWGQYFRNMKRARGGSKSDKDSVQEEGQDSDAEVSFTDEPSMAEMGPANGLYGGLGEPAPALGRPSGAPGSFPLEHGGLAGPEQYGELRPSSPYGVPSSPAALQSLPGPQPLLSSLVYPEAGLGLVPAGPPGGPPPMRVLAGNGPSSDLSTGSSGGYPDFPASPASWLDEVDHAQF | ||||||
Compositional bias | 219-234 | Basic and acidic residues | ||||
Sequence: ARGGSKSDKDSVQEEG | ||||||
Compositional bias | 304-318 | Polar residues | ||||
Sequence: PSSPAALQSLPGPQP |
Domain
The LIM domain specifically interacts with the Pit-1 POU domain and is required for synergistic interactions with Pit-1, but not for basal transcriptional activation events.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusFragment
- Length383
- Mass (Da)41,825
- Last updated1999-05-01 v1
- Checksum0331CC13D8DA0EA7
Features
Showing features for non-terminal residue, compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Non-terminal residue | 1 | |||||
Sequence: W | ||||||
Compositional bias | 219-234 | Basic and acidic residues | ||||
Sequence: ARGGSKSDKDSVQEEG | ||||||
Compositional bias | 304-318 | Polar residues | ||||
Sequence: PSSPAALQSLPGPQP |
Keywords
- Technical term