O94488 · MBO1_SCHPO
- ProteinGamma tubulin complex adapter mto1
- Genemto1
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids1115 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Spindle pole body (SPB) component that acts as the gamma-tubulin complex-binding protein of the SPB outer plaque (PubMed:15120067, PubMed:15659644, PubMed:19001497).
Promotes nucleation of all cytoplasmic microtubules by recruiting the gamma-tubulin complex to the spindle pole body (SPB), to the interphase microtubule organizing center (iMTOC), and to the equatorial MTOC (eMTOC) during anaphase (PubMed:15004232, PubMed:15120067, PubMed:15659644, PubMed:19001497).
Promotes nucleation of all cytoplasmic microtubules by recruiting the gamma-tubulin complex to the spindle pole body (SPB), to the interphase microtubule organizing center (iMTOC), and to the equatorial MTOC (eMTOC) during anaphase (PubMed:15004232, PubMed:15120067, PubMed:15659644, PubMed:19001497).
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Names & Taxonomy
Protein names
- Recommended nameGamma tubulin complex adapter mto1
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Fungi > Dikarya > Ascomycota > Taphrinomycotina > Schizosaccharomycetes > Schizosaccharomycetales > Schizosaccharomycetaceae > Schizosaccharomyces
Accessions
- Primary accessionO94488
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Note: Localizes at the interphase MTOC (iMTOC) and at the equatorial MTOC (eMTOC) at the end of mitosis.
Keywords
- Cellular component
Phenotypes & Variants
Disruption phenotype
Abolishes cytoplasmic microtubule (MT) nucleation from SPB and non-SPB sites (PubMed:15120067, PubMed:19001497).
Leads to MTs escaping from the nucleus to form fewer and thicker cytoplasmic MT bundles that curve around the cell tip (PubMed:15659644, PubMed:19001497).
Curved cell shape (PubMed:15659644, PubMed:19001497).
Leads to unclustering of centromeres at the nuclear periphery; normally, the centromeres cluster at the SPB during interphase (PubMed:17881496).
Leads to MTs escaping from the nucleus to form fewer and thicker cytoplasmic MT bundles that curve around the cell tip (PubMed:15659644, PubMed:19001497).
Curved cell shape (PubMed:15659644, PubMed:19001497).
Leads to unclustering of centromeres at the nuclear periphery; normally, the centromeres cluster at the SPB during interphase (PubMed:17881496).
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Mutagenesis | 259-267 | Abolishes cytoplasmic microtubule (MT) nucleation and leads to MTs escaping from the nucleus to form abnormal MT bundles that curve around the cell tip and a curved cell shape. Abolishes interactions with gamma-tubulin complex components. | |||
Mutagenesis | 267-275 | Abolishes cytoplasmic microtubule (MT) nucleation and leads to MTs escaping from the nucleus to form abnormal MT bundles that curve around the cell tip and a curved cell shape. Abolishes interactions with gamma-tubulin complex components. | |||
Mutagenesis | 288-296 | Mildly curved cell shape. Phenotypically mostly normal. | |||
Mutagenesis | 523-537 | Abolishes interaction with mto2 and with components of the gamma-tubulin complex. Abolishes cytoplasmic microtubule nucleation from non-SPB (spindle pole body) sites, but not from the SPB. Morphologically, cells are curved. | |||
PTM/Processing
Interaction
Subunit
Interacts with mto2; the interaction is direct and required for efficient binding to the gamma-tubulin complex (PubMed:15659644, PubMed:15800064, PubMed:19001497).
Interacts with gamma tubulin complex subunits alp4, alp6 and gtb1 (PubMed:19001497).
Interacts with mcp6 (PubMed:15120067).
Interacts with gamma tubulin complex subunits alp4, alp6 and gtb1 (PubMed:19001497).
Interacts with mcp6 (PubMed:15120067).
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for region, compositional bias, coiled coil.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Region | 25-180 | Disordered | |||
Compositional bias | 30-74 | Basic and acidic residues | |||
Compositional bias | 75-178 | Polar residues | |||
Coiled coil | 445-915 | ||||
Region | 523-537 | Required for interaction with mto2 | |||
Region | 1001-1037 | Disordered | |||
Compositional bias | 1067-1101 | Basic and acidic residues | |||
Region | 1067-1115 | Disordered | |||
Coiled coil | 1072-1102 | ||||
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length1,115
- Mass (Da)128,469
- Last updated1999-05-01 v1
- Checksum8CA5503EC141E3E0
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Compositional bias | 30-74 | Basic and acidic residues | |||
Compositional bias | 75-178 | Polar residues | |||
Compositional bias | 1067-1101 | Basic and acidic residues | |||
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CU329672 EMBL· GenBank· DDBJ | CAA22653.1 EMBL· GenBank· DDBJ | Genomic DNA |