O93922 · ATG7_PICPA

Function

function

E1-like activating enzyme involved in the 2 ubiquitin-like systems required for cytoplasm to vacuole transport (Cvt) and autophagy. Activates ATG12 for its conjugation with ATG5 and ATG8 for its conjugation with phosphatidylethanolamine. Both systems are needed for the ATG8 association to Cvt vesicles and autophagosomes membranes. Autophagy is essential for maintenance of amino acid levels and protein synthesis under nitrogen starvation. Required for selective autophagic degradation of the nucleus (nucleophagy) as well as for mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Plays a role in the regulation of filamentous growth and chronological longevity (By similarity).
Involved in glucose-induced micropexophagy

Features

Showing features for active site.

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TypeIDPosition(s)Description
Active site518Glycyl thioester intermediate

GO annotations

all annotationsall molecular functionvirus receptor activitydna bindingrna bindingcytoskeletal motor activitycatalytic activitygtpase activitystructural molecule activitytransporter activitycytoskeletal protein bindinglipid bindingcyclase activityantioxidant activityoxidoreductase activitytransferase activityhydrolase activitylyase activityisomerase activityligase activityprotein tag activitycargo receptor activityhistone bindingprotein folding chaperonetranslation regulator activitynutrient reservoir activityreceptor ligand activitymolecular transducer activitymolecular adaptor activitytoxin activitycell adhesion mediator activitymolecular function regulator activityvirus coreceptor activitycatalytic activity, acting on a proteincatalytic activity, acting on dnacatalytic activity, acting on rnamolecular carrier activitytranscription regulator activitygeneral transcription initiation factor activitymolecular sensor activitymolecular sequestering activityatp-dependent activityother molecular functionall biological processmitotic cell cyclecytokinesiscytoplasmic translationimmune system processmuscle system processcirculatory system processrenal system processrespiratory system processcarbohydrate metabolic processgeneration of precursor metabolites and energydna replicationdna repairdna recombinationchromatin organizationdna-templated transcriptionregulation of dna-templated transcriptiontrna metabolic processprotein foldingprotein glycosylationamino acid metabolic processmodified amino acid metabolic processlipid metabolic processvitamin metabolic processsulfur compound metabolic processintracellular protein transportnucleocytoplasmic transportautophagyinflammatory responsemitochondrion organizationcytoskeleton organizationmicrotubule-based movementperoxisome organizationlysosome organizationchromosome segregationcell adhesionestablishment or maintenance of cell polarityprogrammed cell deathphotosynthesismrna metabolic processsnrna metabolic processvesicle-mediated transportreproductive processdigestive system processsignalingcell differentiationprotein catabolic processextracellular matrix organizationregulatory ncrna-mediated gene silencingtelomere organizationcell junction organizationwound healingribosome biogenesiscilium organizationanatomical structure developmentcell motilitynervous system processendocrine processprotein maturationtransmembrane transportnucleobase-containing small molecule metabolic processhepaticobiliary system processmembrane organizationprotein-containing complex assemblycell wall organization or biogenesisnitrogen cycle metabolic processprotein localization to plasma membranedefense response to other organismdetoxificationmeiotic nuclear divisionmitotic nuclear divisionmitochondrial gene expressioncarbohydrate derivative metabolic processother biological processall cellular componentnuclear chromosomeextracellular regionextracellular spacecell wallnucleusnuclear envelopenucleoplasmchromosomenucleolusmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuslipid dropletmicrotubule organizing centercytosolribosomecytoskeletonplasma membraneciliumplastidthylakoidexternal encapsulating structureextracellular matrixcytoplasmic vesicleorganelleother cellular component
Cell color indicative of number of GO terms
AspectTerm
Cellular Componentphagophore assembly site
Molecular FunctionAtg12 activating enzyme activity
Molecular FunctionAtg8 activating enzyme activity
Biological Processautophagosome assembly
Biological Processautophagy of mitochondrion
Biological Processcellular response to nitrogen starvation
Biological Processmicropexophagy
Biological Processpexophagy
Biological Processpiecemeal microautophagy of the nucleus
Biological Processprotein modification by small protein conjugation
Biological Processprotein transport

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    Ubiquitin-like modifier-activating enzyme ATG7
  • Alternative names
    • ATG12-activating enzyme E1 ATG7
    • Autophagy-related protein 7
    • Glucose-induced selective autophagy protein 7
    • Pexophagy zeocin-resistant mutant protein 12

Gene names

    • Name
      ATG7
    • Synonyms
      GSA7, PAZ12

Organism names

Accessions

  • Primary accession
    O93922

Subcellular Location

Keywords

Phenotypes & Variants

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis518No more micropexophagy.
Mutagenesis562Normal micropexophagy.

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00002128191-654Ubiquitin-like modifier-activating enzyme ATG7

Interaction

Subunit

Homodimer. Interacts with ATG8 through a thioester bond between Cys-518 and the C-terminal 'Gly-116' of ATG8 (By similarity).

Structure

Family & Domains

Features

Showing features for motif, region.

TypeIDPosition(s)Description
Motif332-337GXGXXG motif
Region615-654Homodimerization

Domain

The C-terminal 40 residues are required for homodimerization.
The GxGxxG motif is important for the function, possibly through binding with ATP.

Sequence similarities

Belongs to the ATG7 family.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    654
  • Mass (Da)
    74,293
  • Last updated
    1999-05-01 v1
  • Checksum
    893937F5FBB30D8F
MTSMDIPYSQISSFVNSSFFQKVSQLKLNKYRLDDTDKAIVGSVDFKFIGKNQPTSLSVDESSFNDNITYTHAQFPVKGILKNLNTVEDFRKVDKNEFLQSQGLVVHKSIQDRSCLKDLSKLTQFFILSFSDLKGFKFIYWFGFPSLVSRWKVNKLSGLTESQIEPYESKLNEWLNARLPIEQKQAFIIDNLEFKPFEQLSSFSPDDQLNIGFIDTSSILNKCSTQLRNILYMLAYYGFENIKVYNFRFNNTTSFTLDITLAEPLTSEPKTTGWERTAQGKLGPKLADIGALVDPARLADQSVDLNLKLMKWRVMPELDLDIIKNSKVLLLGAGTLGSYVSRVLLGYGVRHITFVDNGKVSFSNPVRQPLFNFTDCLEGGAPKAETAAKALKLIFPLITSQGYNLEVPMAGHPVTDEKRQYEDYQRLVTLIKEHDVVFLLMDSRETRWLPTVLCNVFDKICITAALGFDSYLVMRHGNLFNTEHIEAEENSHRLGCYFCNDIIAPKDSTTDRTLDQMCTVTRPGVALLASSLAAELFVSILQHPLKSHAPASLHDNATVLGCLPQQLRGFLHNFETSKLEANNYEYCSACSIQVLNEYKSRTWDFVKDALNENNYLEDLTGLTKVKQESEIAEKKFQEFENGLEFSDEDSEWIN

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AF098976
EMBL· GenBank· DDBJ
AAD14610.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

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