O93801 · AKT2_ALTAL

Function

function

Abhydrolase domain-containing protein; part of the gene clusters that mediate the biosynthesis of the host-selective toxins (HSTs) AK-toxins responsible for Japanese pear black spot disease by the Japanese pear pathotype (PubMed:10432635, PubMed:20348386).
AK-toxins are esters of 9,10-epoxy 8-hydroxy 9-methyldecatrienoic acid (EDA) (PubMed:22846083).
On cellular level, AK-toxins affect plasma membrane of susceptible cells and cause a sudden increase in loss of K+ after a few minutes of toxin treatment (PubMed:22846083).
The acyl-CoA ligase AKT1, the hydrolase AKT2 and enoyl-CoA hydratase AKT3 are all involved in the biosynthesis of the AK-, AF- and ACT-toxin common 9,10-epoxy-8-hydroxy-9-methyl-decatrienoic acid (EDA) structural moiety (PubMed:10432635, PubMed:10975654, PubMed:22846083).
Part of the EDA biosynthesis occurs in the peroxisome since these 3 enzymes are localized in peroxisomes (PubMed:20348386).
The exact roles of the 3 enzymes, as well as of additional AK-toxin clusters enzymes, including AKT4, AKT6 and AKTS1, have still to be elucidated (PubMed:10432635, PubMed:10975654, PubMed:22846083).
The Cytochrome P450 monooxygenase AKT7 on the other side functions to limit production of EDA and AK-toxin, probably via the catalysis of a side reaction of EDA or its precursor (PubMed:24611558).

Miscellaneous

Gene clusters encoding host-selective toxins (HSTs) are localized on conditionally dispensable chromosomes (CDCs), also called supernumerary chromosomes, where they are present in multiple copies (PubMed:10975654).
The CDCs are not essential for saprophytic growth but controls host-selective pathogenicity (PubMed:10975654).

Pathway

Mycotoxin biosynthesis.

GO annotations

AspectTerm
Cellular Componentperoxisome
Molecular Functionhydrolase activity

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Abhydrolase domain-containing protein AKT2
  • EC number
  • Alternative names
    • AF-toxin biosynthesis protein 2

Gene names

    • Name
      AKT2
    • Synonyms
      AKT2-1

Organism names

Accessions

  • Primary accession
    O93801

Organism-specific databases

Subcellular Location

Peroxisome
Note: The peroxisomal location requires the C-terminal tripeptide peroxisomal targeting signal.

Keywords

Phenotypes & Variants

Disruption phenotype

Abolishes the production of AK-toxin and impairs the pathogenicity.

Miscellaneous

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00004448251-262Abhydrolase domain-containing protein AKT2

Structure

Family & Domains

Features

Showing features for motif.

TypeIDPosition(s)Description
Motif260-262Peroxisomal targeting signal type 1

Sequence similarities

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    262
  • Mass (Da)
    29,432
  • Last updated
    1999-05-01 v1
  • MD5 Checksum
    E2A8A3DA2303FC6350DF8244F74B13B6
MQQPIIGVGHSMGGCQIATLSVTSRRMFSTMILLDPAIGPPDMGLATLDLGQLTLRRRTQWPTREDAEKALRTSFSTWDSQVLNLLIKHSIHSDKQSIEMEDGPVSLVTGRYQELVNYIKPSFIRSGKVNGQELIHQTGPVDMYHMLGLVTCSALYLCGGESTLSVPRVRDLWLNRTAKLSYSKEPGETRKVDERIVPDTGHFLPMEEPKKCADIIADWIEKDKCIAWNCCVGKRGKTWCELSNASKEMSAEAWMKYLQSKL

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AB015352
EMBL· GenBank· DDBJ
BAA36589.1
EMBL· GenBank· DDBJ
Genomic DNA
AB872924
EMBL· GenBank· DDBJ
BAO10614.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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