O88898 · P63_MOUSE
- ProteinTumor protein 63
- GeneTp63
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids680 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Acts as a sequence specific DNA binding transcriptional activator or repressor. The isoforms contain a varying set of transactivation and auto-regulating transactivation inhibiting domains thus showing an isoform specific activity. May be required in conjunction with TP73/p73 for initiation of p53/TP53 dependent apoptosis in response to genotoxic insults and the presence of activated oncogenes. Involved in Notch signaling by probably inducing JAG1 and JAG2. Activates transcription of the p21 promoter (By similarity).
Activates RIPK4 transcription. Plays a role in the regulation of epithelial morphogenesis. The ratio of DeltaN-type and TA*-type isoforms may govern the maintenance of epithelial stem cell compartments and regulate the initiation of epithelial stratification from the undifferentiated embryonal ectoderm. Required for limb formation from the apical ectodermal ridge
Activates RIPK4 transcription. Plays a role in the regulation of epithelial morphogenesis. The ratio of DeltaN-type and TA*-type isoforms may govern the maintenance of epithelial stem cell compartments and regulate the initiation of epithelial stratification from the undifferentiated embryonal ectoderm. Required for limb formation from the apical ectodermal ridge
Cofactor
Note: Binds 1 zinc ion per subunit.
Features
Showing features for dna binding, binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
DNA binding | 170-362 | |||||
Sequence: DYPGPHSFDVSFQQSSTAKSATWTYSTELKKLYCQIAKTCPIQIKVMTPPPQGAVIRAMPVYKKAEHVTEVVKRCPNHELSREFNEGQIAPPSHLIRVEGNSHAQYVEDPITGRQSVLVPYEPPQVGTEFTTVLYNFMCNSSCVGGMNRRPILIIVTLETRDGQVLGRRCFEARICACPGRDRKADEDSIRKQ | ||||||
Binding site | 244 | Zn2+ (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 247 | Zn2+ (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Binding site | 308 | Zn2+ (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 312 | Zn2+ (UniProtKB | ChEBI) | ||||
Sequence: C |
GO annotations
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameTumor protein 63
- Short namesp63
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Mus > Mus
Accessions
- Primary accessionO88898
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
Phenotypes & Variants
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 543 | Abolishes interaction with WWP1. | ||||
Sequence: Y → F | ||||||
Mutagenesis | 647 | Heterozygous mutant mice have reduced fertility, reduced ovary size and accelerated oocyte loss via apoptosis. | ||||
Sequence: R → C |
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 28 variants from UniProt as well as other sources including ClinVar and dbSNP.
PTM/Processing
Features
Showing features for chain, cross-link.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000185730 | 1-680 | Tumor protein 63 | |||
Sequence: MNFETSRCATLQYCPDPYIQRFIETPAHFSWKESYYRSAMSQSTQTSEFLSPEVFQHIWDFLEQPICSVQPIELNFVDEPSENGATNKIEISMDCIRMQDSDLSDPMWPQYTNLGLLNSMDQQIQNGSSSTSPYNTDHAQNSVTAPSPYAQPSSTFDALSPSPAIPSNTDYPGPHSFDVSFQQSSTAKSATWTYSTELKKLYCQIAKTCPIQIKVMTPPPQGAVIRAMPVYKKAEHVTEVVKRCPNHELSREFNEGQIAPPSHLIRVEGNSHAQYVEDPITGRQSVLVPYEPPQVGTEFTTVLYNFMCNSSCVGGMNRRPILIIVTLETRDGQVLGRRCFEARICACPGRDRKADEDSIRKQQVSDSAKNGDGTKRPFRQNTHGIQMTSIKKRRSPDDELLYLPVRGRETYEMLLKIKESLELMQYLPQHTIETYRQQQQQQHQHLLQKQTSMQSQSSYGNSSPPLNKMNSMNKLPSVSQLINPQQRNALTPTTMPEGMGANIPMMGTHMPMAGDMNGLSPTQALPPPLSMPSTSHCTPPPPYPTDCSIVSFLARLGCSSCLDYFTTQGLTTIYQIEHYSMDDLASLKIPEQFRHAIWKGILDHRQLHDFSSPPHLLRTPSGASTVSVGSSETRGERVIDAVRFTLRQTISFPPRDEWNDFNFDMDSRRNKQQRIKEEGE | ||||||
Cross-link | 676 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO) | ||||
Sequence: K |
Post-translational modification
May be sumoylated.
Ubiquitinated. Polyubiquitination involves WWP1 and leads to proteasomal degradation of this protein.
Keywords
- PTM
Proteomic databases
PTM databases
Expression
Tissue specificity
Widely expressed, notably in thymus, prostate, placenta and skeletal muscle, although the precise isoform varies according to tissue type. Progenitor cell layers of skin, breast and prostate express high levels of DeltaN-type isoforms.
Induction
Induced by DNA damaging agents.
Developmental stage
Expressed in the basal layer and a small number of cells in the spinous layer of the tongue at P20.
Isoform 1
Expressed in the epidermis from 7.5 dpc, prior to onset of epithelial stratification.
Isoform 2
Expressed in the epidermis from 9.5 dpc, after the onset of epithelial stratification but prior to terminal differentiation.
Isoform 3
Expressed in the epidermis from 7.5 dpc, prior to onset of epithelial stratification.
Isoform 4
Expressed in the epidermis from 9.5 dpc, after the onset of epithelial stratification but prior to terminal differentiation.
Isoform 5
Expressed in the epidermis from 7.5 dpc, prior to onset of epithelial stratification.
Isoform 6
Expressed in the epidermis from 9.5 dpc, after the onset of epithelial stratification but prior to terminal differentiation.
Gene expression databases
Interaction
Subunit
Binds DNA as a homotetramer. Isoform composition of the tetramer may determine transactivation activity. Interacts with HIPK2. Interacts with SSRP1, leading to stimulate coactivator activity. Interacts with PDS5A. Interacts (via activation domain) with NOC2L (By similarity).
Interacts with WWP1
Interacts with WWP1
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | Intact | |
---|---|---|---|---|---|
BINARY | O88898 | Cables1 Q9ESJ1 | 2 | EBI-2338025, EBI-604411 | |
XENO | O88898 | TP53 P04637 | 2 | EBI-2338025, EBI-366083 | |
BINARY | O88898 | Tp63 O88898 | 2 | EBI-2338025, EBI-2338025 | |
BINARY | O88898-1 | Tp63 O88898-1 | 19 | EBI-3863990, EBI-3863990 | |
XENO | O88898-2 | KAT2B Q92831 | 3 | EBI-2338228, EBI-477430 | |
BINARY | O88898-2 | Tp63 O88898-2 | 2 | EBI-2338228, EBI-2338228 | |
BINARY | O88898-5 | Tp63 O88898-5 | 5 | EBI-2338240, EBI-2338240 |
Protein-protein interaction databases
Miscellaneous
Structure
Family & Domains
Features
Showing features for region, compositional bias, domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 1-107 | Transcription activation | ||||
Sequence: MNFETSRCATLQYCPDPYIQRFIETPAHFSWKESYYRSAMSQSTQTSEFLSPEVFQHIWDFLEQPICSVQPIELNFVDEPSENGATNKIEISMDCIRMQDSDLSDPM | ||||||
Compositional bias | 123-166 | Polar residues | ||||
Sequence: QIQNGSSSTSPYNTDHAQNSVTAPSPYAQPSSTFDALSPSPAIP | ||||||
Region | 123-171 | Disordered | ||||
Sequence: QIQNGSSSTSPYNTDHAQNSVTAPSPYAQPSSTFDALSPSPAIPSNTDY | ||||||
Region | 351-393 | Disordered | ||||
Sequence: DRKADEDSIRKQQVSDSAKNGDGTKRPFRQNTHGIQMTSIKKR | ||||||
Region | 352-388 | Interaction with HIPK2 | ||||
Sequence: RKADEDSIRKQQVSDSAKNGDGTKRPFRQNTHGIQMT | ||||||
Compositional bias | 364-389 | Polar residues | ||||
Sequence: VSDSAKNGDGTKRPFRQNTHGIQMTS | ||||||
Region | 394-443 | Oligomerization | ||||
Sequence: RSPDDELLYLPVRGRETYEMLLKIKESLELMQYLPQHTIETYRQQQQQQH | ||||||
Region | 436-472 | Disordered | ||||
Sequence: RQQQQQQHQHLLQKQTSMQSQSSYGNSSPPLNKMNSM | ||||||
Domain | 541-607 | SAM | ||||
Sequence: PPYPTDCSIVSFLARLGCSSCLDYFTTQGLTTIYQIEHYSMDDLASLKIPEQFRHAIWKGILDHRQL | ||||||
Region | 610-680 | Transactivation inhibition | ||||
Sequence: FSSPPHLLRTPSGASTVSVGSSETRGERVIDAVRFTLRQTISFPPRDEWNDFNFDMDSRRNKQQRIKEEGE |
Domain
The transactivation inhibitory domain (TID) can interact with, and inhibit the activity of the N-terminal transcriptional activation domain of TA*-type isoforms.
Sequence similarities
Belongs to the p53 family.
Phylogenomic databases
Family and domain databases
Sequence & Isoforms
- Sequence statusComplete
This entry describes 6 isoforms produced by Alternative promoter usage & Alternative splicing.
O88898-1
This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
- Name1
- SynonymsTA*-alpha, TA*p63alpha
- NoteProduced by alternative promoter usage.
- Length680
- Mass (Da)76,789
- Last updated1998-11-01 v1
- Checksum8DFF0284F247C68A
O88898-2
- Name2
- SynonymsDeltaN-alpha
- NoteProduced by alternative promoter usage.
- Differences from canonical
- 1-108: MNFETSRCATLQYCPDPYIQRFIETPAHFSWKESYYRSAMSQSTQTSEFLSPEVFQHIWDFLEQPICSVQPIELNFVDEPSENGATNKIEISMDCIRMQDSDLSDPMW → MLYLENNAQTQFSE
O88898-3
- Name3
- SynonymsTA*-beta, TA*p63beta
- NoteProduced by alternative splicing of isoform 1.
- Differences from canonical
- 551-680: SFLARLGCSSCLDYFTTQGLTTIYQIEHYSMDDLASLKIPEQFRHAIWKGILDHRQLHDFSSPPHLLRTPSGASTVSVGSSETRGERVIDAVRFTLRQTISFPPRDEWNDFNFDMDSRRNKQQRIKEEGE → RIWQV
O88898-4
- Name4
- SynonymsDeltaN-beta
- NoteProduced by alternative splicing of isoform 2.
- Differences from canonical
O88898-5
- Name5
- SynonymsTA*-gamma, TA*p63gamma
- NoteProduced by alternative splicing of isoform 1.
- Differences from canonical
O88898-6
- Name6
- SynonymsDeltaN-gamma
- NoteProduced by alternative splicing of isoform 2.
- Differences from canonical
- 1-108: MNFETSRCATLQYCPDPYIQRFIETPAHFSWKESYYRSAMSQSTQTSEFLSPEVFQHIWDFLEQPICSVQPIELNFVDEPSENGATNKIEISMDCIRMQDSDLSDPMW → MLYLENNAQTQFSE
- 373-377: GTKRP → A
- 450-680: QTSMQSQSSYGNSSPPLNKMNSMNKLPSVSQLINPQQRNALTPTTMPEGMGANIPMMGTHMPMAGDMNGLSPTQALPPPLSMPSTSHCTPPPPYPTDCSIVSFLARLGCSSCLDYFTTQGLTTIYQIEHYSMDDLASLKIPEQFRHAIWKGILDHRQLHDFSSPPHLLRTPSGASTVSVGSSETRGERVIDAVRFTLRQTISFPPRDEWNDFNFDMDSRRNKQQRIKEEGE → HLLSACFRNELVEPRGEAPTQSDVFFRHSNPPNHSVYP
Computationally mapped potential isoform sequences
There are 4 potential isoforms mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
A0A0R4J1K8 | A0A0R4J1K8_MOUSE | Trp63 | 284 | ||
Q569E5 | Q569E5_MOUSE | Trp63 | 393 | ||
D3Z7K2 | D3Z7K2_MOUSE | Trp63 | 231 | ||
Q3UVI3 | Q3UVI3_MOUSE | Trp63 | 582 |
Sequence caution
Features
Showing features for alternative sequence, compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Alternative sequence | VSP_012471 | 1-108 | in isoform 2, isoform 4 and isoform 6 | |||
Sequence: MNFETSRCATLQYCPDPYIQRFIETPAHFSWKESYYRSAMSQSTQTSEFLSPEVFQHIWDFLEQPICSVQPIELNFVDEPSENGATNKIEISMDCIRMQDSDLSDPMW → MLYLENNAQTQFSE | ||||||
Compositional bias | 123-166 | Polar residues | ||||
Sequence: QIQNGSSSTSPYNTDHAQNSVTAPSPYAQPSSTFDALSPSPAIP | ||||||
Compositional bias | 364-389 | Polar residues | ||||
Sequence: VSDSAKNGDGTKRPFRQNTHGIQMTS | ||||||
Alternative sequence | VSP_012472 | 373-377 | in isoform 5 and isoform 6 | |||
Sequence: GTKRP → A | ||||||
Alternative sequence | VSP_012473 | 450-680 | in isoform 5 and isoform 6 | |||
Sequence: QTSMQSQSSYGNSSPPLNKMNSMNKLPSVSQLINPQQRNALTPTTMPEGMGANIPMMGTHMPMAGDMNGLSPTQALPPPLSMPSTSHCTPPPPYPTDCSIVSFLARLGCSSCLDYFTTQGLTTIYQIEHYSMDDLASLKIPEQFRHAIWKGILDHRQLHDFSSPPHLLRTPSGASTVSVGSSETRGERVIDAVRFTLRQTISFPPRDEWNDFNFDMDSRRNKQQRIKEEGE → HLLSACFRNELVEPRGEAPTQSDVFFRHSNPPNHSVYP | ||||||
Alternative sequence | VSP_012474 | 551-680 | in isoform 3 and isoform 4 | |||
Sequence: SFLARLGCSSCLDYFTTQGLTTIYQIEHYSMDDLASLKIPEQFRHAIWKGILDHRQLHDFSSPPHLLRTPSGASTVSVGSSETRGERVIDAVRFTLRQTISFPPRDEWNDFNFDMDSRRNKQQRIKEEGE → RIWQV |
Keywords
- Coding sequence diversity
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AF075434 EMBL· GenBank· DDBJ | AAC62639.1 EMBL· GenBank· DDBJ | mRNA | ||
AF075435 EMBL· GenBank· DDBJ | AAC62640.1 EMBL· GenBank· DDBJ | mRNA | ||
AF075436 EMBL· GenBank· DDBJ | AAC62641.1 EMBL· GenBank· DDBJ | mRNA | ||
AF075437 EMBL· GenBank· DDBJ | AAC62642.1 EMBL· GenBank· DDBJ | mRNA | ||
AF075438 EMBL· GenBank· DDBJ | AAC62643.1 EMBL· GenBank· DDBJ | mRNA | ||
AF075439 EMBL· GenBank· DDBJ | AAC62644.1 EMBL· GenBank· DDBJ | mRNA | ||
AB010152 EMBL· GenBank· DDBJ | BAA32432.1 EMBL· GenBank· DDBJ | mRNA | ||
AF533892 EMBL· GenBank· DDBJ | AAP87982.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AF533892 EMBL· GenBank· DDBJ | AAP87985.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AF533892 EMBL· GenBank· DDBJ | AAP87983.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AF533892 EMBL· GenBank· DDBJ | AAP87984.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AF533892 EMBL· GenBank· DDBJ | AAP87986.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AF533892 EMBL· GenBank· DDBJ | AAP87987.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AK048623 EMBL· GenBank· DDBJ | BAC33397.1 EMBL· GenBank· DDBJ | mRNA | Sequence problems. |