O88838 · SPSB2_MOUSE
- ProteinSPRY domain-containing SOCS box protein 2
- GeneSpsb2
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids264 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Substrate recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins (PubMed:20603330).
Negatively regulates nitric oxide (NO) production and limits cellular toxicity in activated macrophages by mediating the ubiquitination and proteasomal degradation of NOS2 (PubMed:20603330).
Acts as a bridge which links NOS2 with the ECS E3 ubiquitin ligase complex components ELOC and CUL5 (By similarity).
Negatively regulates nitric oxide (NO) production and limits cellular toxicity in activated macrophages by mediating the ubiquitination and proteasomal degradation of NOS2 (PubMed:20603330).
Acts as a bridge which links NOS2 with the ECS E3 ubiquitin ligase complex components ELOC and CUL5 (By similarity).
Pathway
Protein modification; protein ubiquitination.
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Cellular Component | SCF ubiquitin ligase complex | |
Molecular Function | ubiquitin-like ligase-substrate adaptor activity | |
Biological Process | intracellular signal transduction | |
Biological Process | proteasome-mediated ubiquitin-dependent protein catabolic process | |
Biological Process | protein ubiquitination | |
Biological Process | ubiquitin-dependent protein catabolic process |
Keywords
- Biological process
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameSPRY domain-containing SOCS box protein 2
- Short namesSSB-2
- Alternative names
Gene names
Organism names
- Organism
- Strains
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Mus > Mus
Accessions
- Primary accessionO88838
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Note: Exhibits a diffuse cytosolic localization.
Keywords
- Cellular component
Phenotypes & Variants
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 100-101 | Loss of interaction with NOS2. | ||||
Sequence: RG → AA | ||||||
Mutagenesis | 102-103 | Significant loss of interaction with NOS2. | ||||
Sequence: TH → AA | ||||||
Mutagenesis | 118-119 | No loss of interaction with NOS2. | ||||
Sequence: DH → AA | ||||||
Mutagenesis | 120 | Loss of interaction with PARW. No effect on interaction with MET. Loss of interaction with NOS2. | ||||
Sequence: Y → A | ||||||
Mutagenesis | 120 | Loss of interaction with NOS2. | ||||
Sequence: Y → F | ||||||
Mutagenesis | 123-125 | Loss of interaction with MET and PARW. Loss of interaction with NOS2. | ||||
Sequence: LLG → AAA | ||||||
Mutagenesis | 126-128 | Loss of interaction with PAWR. Significant loss of interaction with NOS2. | ||||
Sequence: SNS → AAA | ||||||
Mutagenesis | 141-143 | Strongly reduced interaction with MET and PARW. | ||||
Sequence: YHQ → AAA | ||||||
Mutagenesis | 151-152 | Loss of interaction with MET and PARW. | ||||
Sequence: QY → AA | ||||||
Mutagenesis | 160-161 | No loss of interaction with NOS2. | ||||
Sequence: QL → AA | ||||||
Mutagenesis | 188-189 | Strongly reduced interaction with MET. Loss of interaction with PARW. | ||||
Sequence: GP → AA | ||||||
Mutagenesis | 198 | No loss of interaction with PAWR. | ||||
Sequence: T → A | ||||||
Mutagenesis | 206 | Loss of interaction with PARW. No effect on interaction with MET. Loss of interaction with NOS2. | ||||
Sequence: V → A | ||||||
Mutagenesis | 207 | Significant loss of interaction with NOS2. | ||||
Sequence: W → A |
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 10 variants from UniProt as well as other sources including ClinVar and dbSNP.
Chemistry
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000238475 | 1-264 | SPRY domain-containing SOCS box protein 2 | |||
Sequence: MGQTALARGSSSTPTSQALYSDFSPPEGLEELLSAPPPDLVAQRHHGWNPKDCSENIDVKEGGLCFERRPVAQSTDGVRGKRGYSRGLHAWEISWPLEQRGTHAVVGVATALAPLQADHYAALLGSNSESWGWDIGRGKLYHQSKGLEAPQYPAGPQGEQLVVPERLLVVLDMEEGTLGYSIGGTYLGPAFRGLKGRTLYPSVSAVWGQCQVRIRYMGERRVEEPQSLLHLSRLCVRHALGDTRLGQISTLPLPPAMKRYLLYK |
Proteomic databases
PTM databases
Expression
Gene expression databases
Interaction
Subunit
Component of the probable ECS(SPSB2) E3 ubiquitin-protein ligase complex which contains CUL5, RNF7/RBX2, Elongin BC complex and SPSB2 (By similarity).
Interacts with CUL5, RNF7, ELOB and ELOC (By similarity).
Interacts with MET (PubMed:16369487).
Interacts (via B30.2/SPRY domain) with PAWR; this interaction occurs in association with the Elongin BC complex (PubMed:16369487, PubMed:20561531).
Interacts with NOS2
Interacts with CUL5, RNF7, ELOB and ELOC (By similarity).
Interacts with MET (PubMed:16369487).
Interacts (via B30.2/SPRY domain) with PAWR; this interaction occurs in association with the Elongin BC complex (PubMed:16369487, PubMed:20561531).
Interacts with NOS2
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | Intact | |
---|---|---|---|---|---|
XENO | O88838 | PAWR Q96IZ0 | 6 | EBI-8820410, EBI-595869 |
Protein-protein interaction databases
Chemistry
Miscellaneous
Structure
Family & Domains
Features
Showing features for compositional bias, region, domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 1-22 | Polar residues | ||||
Sequence: MGQTALARGSSSTPTSQALYSD | ||||||
Region | 1-34 | Disordered | ||||
Sequence: MGQTALARGSSSTPTSQALYSDFSPPEGLEELLS | ||||||
Domain | 26-221 | B30.2/SPRY | ||||
Sequence: PEGLEELLSAPPPDLVAQRHHGWNPKDCSENIDVKEGGLCFERRPVAQSTDGVRGKRGYSRGLHAWEISWPLEQRGTHAVVGVATALAPLQADHYAALLGSNSESWGWDIGRGKLYHQSKGLEAPQYPAGPQGEQLVVPERLLVVLDMEEGTLGYSIGGTYLGPAFRGLKGRTLYPSVSAVWGQCQVRIRYMGERR | ||||||
Domain | 222-264 | SOCS box | ||||
Sequence: VEEPQSLLHLSRLCVRHALGDTRLGQISTLPLPPAMKRYLLYK |
Domain
The SOCS box domain mediates the interaction with the Elongin BC complex, an adapter module in different E3 ubiquitin ligase complexes (By similarity).
Essential for its ability to link NOS2 and the ECS E3 ubiquitin ligase complex components ELOC and CUL5 (By similarity).
Essential for its ability to link NOS2 and the ECS E3 ubiquitin ligase complex components ELOC and CUL5 (By similarity).
Sequence similarities
Belongs to the SPSB family.
Phylogenomic databases
Family and domain databases
Sequence & Isoform
- Sequence statusComplete
This entry describes 2 isoforms produced by Alternative splicing.
O88838-1
This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
- Name1
- Length264
- Mass (Da)28,938
- Last updated1998-11-01 v1
- ChecksumE71985597D9B38CB
O88838-2
- Name2
- Differences from canonical
- 222-264: VEEPQSLLHLSRLCVRHALGDTRLGQISTLPLPPAMKRYLLYK → GEIRTRCGEITTLGNGLGWKLMVGAQEVGFLSLWPVT
Computationally mapped potential isoform sequences
There are 2 potential isoforms mapped to this entry
Features
Showing features for compositional bias, alternative sequence.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 1-22 | Polar residues | ||||
Sequence: MGQTALARGSSSTPTSQALYSD | ||||||
Alternative sequence | VSP_018613 | 222-264 | in isoform 2 | |||
Sequence: VEEPQSLLHLSRLCVRHALGDTRLGQISTLPLPPAMKRYLLYK → GEIRTRCGEITTLGNGLGWKLMVGAQEVGFLSLWPVT |
Keywords
- Coding sequence diversity
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AC002397 EMBL· GenBank· DDBJ | AAC36017.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AF403037 EMBL· GenBank· DDBJ | AAL57356.1 EMBL· GenBank· DDBJ | mRNA | ||
AK036451 EMBL· GenBank· DDBJ | BAC29435.1 EMBL· GenBank· DDBJ | mRNA | ||
AK148177 EMBL· GenBank· DDBJ | BAE28397.1 EMBL· GenBank· DDBJ | mRNA | ||
BC002005 EMBL· GenBank· DDBJ | AAH02005.1 EMBL· GenBank· DDBJ | mRNA | ||
BC010305 EMBL· GenBank· DDBJ | AAH10305.1 EMBL· GenBank· DDBJ | mRNA |