O88039 · RAMA_STRCO
- ProteinABC transporter ATP-binding protein RamA
- GeneramA
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids636 (go to sequence)
- Protein existenceEvidence at transcript level
- Annotation score3/5
Function
function
Probably involved in exporting SapB from the cell (Probable). Expression of the ram locus (ramA, ramB and ramR) induces rapid aerial mycelium formation in S.lividans (PubMed:8206859).
Features
Showing features for binding site.
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | plasma membrane | |
Molecular Function | ABC-type transporter activity | |
Molecular Function | ATP binding | |
Molecular Function | ATP hydrolysis activity | |
Molecular Function | ATPase-coupled lipid transmembrane transporter activity | |
Biological Process | transmembrane transport |
Keywords
- Biological process
- Ligand
Names & Taxonomy
Protein names
- Recommended nameABC transporter ATP-binding protein RamA
Gene names
Organism names
- Strains
- Taxonomic lineageBacteria > Actinomycetota > Actinomycetes > Kitasatosporales > Streptomycetaceae > Streptomyces > Streptomyces albidoflavus group
Accessions
- Primary accessionO88039
- Secondary accessions
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Cell membrane ; Multi-pass membrane protein
Features
Showing features for transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Transmembrane | 45-65 | Helical | ||||
Sequence: VLVLLCSVAAAVAAVAQPLAL | ||||||
Transmembrane | 78-98 | Helical | ||||
Sequence: AGWWLPLSAALLLGELLLDSA | ||||||
Transmembrane | 175-195 | Helical | ||||
Sequence: LVDVWVALCVLTGLPALALLL | ||||||
Transmembrane | 269-289 | Helical | ||||
Sequence: GVLVPLLTLAATAVGGLRLAA | ||||||
Transmembrane | 297-317 | Helical | ||||
Sequence: LLAVGRYAQLTAGVGAAASLL |
Keywords
- Cellular component
Phenotypes & Variants
Disruption phenotype
Deletion of the ramC-ramS-ramA-ramB operon on rich medium leads to an initially bald (no aerial hyphae) phenotype; after 4 days develops a substantial aerial mycelium. No expression of SapB, normal expression of chaplins. Wild-type mycelium on minimal medium. A complete chaplin-negative plus ram-negative strain (deletion of ramR or the ramC-ramS-ramA-ramB operon) leads to the complete loss of robust aerial hyphae.
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000445441 | 1-636 | ABC transporter ATP-binding protein RamA | |||
Sequence: MSPAAGAPGSGPGRRRLPALDVRRFSPVRKSRPVPPRAPGRALEVLVLLCSVAAAVAAVAQPLALGRTLDLLLRDGDAGWWLPLSAALLLGELLLDSATSLFTGRCNATWTASVRTRALRGLLRTAPEHARPYPPGDIGTRLTLNAADAGGAPAARAALAASLITPLGALVALALVDVWVALCVLTGLPALALLLRSFARDTGATVAAYQRTQSLIASRLLEALEGADTIGAAGTGERERARVLAPLAELAAQGRHMWALHGRALGRSGVLVPLLTLAATAVGGLRLAAGELSVGDLLAVGRYAQLTAGVGAAASLLGAIVRAREARRRTRELERMTATVYGTRRLPPNGPGELRLCGVRVLRGGREVLRADGVRVPGGSTVAVVGRSGAGKSVLAAVAGRLIDPDEGYVLLDGVRLDRLTHEALRTEVAYAFERPVLGEGTIAEAVADGARRSSRERVRQAARAAGADGFVRRLPHGYDTPLPRAPLSGGEHQRLGLARAFAHAGRLLVLDDATSSLDTATEHEVDLALRRSVRPGTRLVVAHRPSVADRADLVLWLEDGQVRAVGTHRELWHTAGYREVFGAGAGAGAGAGAGAGADAGAGADAGPGPDSGAATAVGGSGPGPVRRPEPEEARP |
Proteomic databases
Expression
Induction
Probably part of the ramC-ramS-ramA-ramB operon.
Interaction
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for domain, region.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 45-322 | ABC transmembrane type-1 | ||||
Sequence: VLVLLCSVAAAVAAVAQPLALGRTLDLLLRDGDAGWWLPLSAALLLGELLLDSATSLFTGRCNATWTASVRTRALRGLLRTAPEHARPYPPGDIGTRLTLNAADAGGAPAARAALAASLITPLGALVALALVDVWVALCVLTGLPALALLLRSFARDTGATVAAYQRTQSLIASRLLEALEGADTIGAAGTGERERARVLAPLAELAAQGRHMWALHGRALGRSGVLVPLLTLAATAVGGLRLAAGELSVGDLLAVGRYAQLTAGVGAAASLLGAIVR | ||||||
Domain | 354-585 | ABC transporter | ||||
Sequence: LRLCGVRVLRGGREVLRADGVRVPGGSTVAVVGRSGAGKSVLAAVAGRLIDPDEGYVLLDGVRLDRLTHEALRTEVAYAFERPVLGEGTIAEAVADGARRSSRERVRQAARAAGADGFVRRLPHGYDTPLPRAPLSGGEHQRLGLARAFAHAGRLLVLDDATSSLDTATEHEVDLALRRSVRPGTRLVVAHRPSVADRADLVLWLEDGQVRAVGTHRELWHTAGYREVFGAG | ||||||
Region | 589-636 | Disordered | ||||
Sequence: GAGAGAGAGADAGAGADAGPGPDSGAATAVGGSGPGPVRRPEPEEARP |
Sequence similarities
Belongs to the ABC transporter superfamily.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length636
- Mass (Da)65,481
- Last updated1998-11-01 v1
- Checksum04CA52FE1135E079
Features
Showing features for sequence conflict.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Sequence conflict | 372 | in Ref. 1; AAA21388 | ||||
Sequence: D → G |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
U03771 EMBL· GenBank· DDBJ | AAA21388.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AL939128 EMBL· GenBank· DDBJ | CAA19962.1 EMBL· GenBank· DDBJ | Genomic DNA |