O85501 · NDK_MYCS2
- ProteinNucleoside diphosphate kinase
- Genendk
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids139 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate.
Catalytic activity
- a 2'-deoxyribonucleoside 5'-diphosphate + ATP = a 2'-deoxyribonucleoside 5'-triphosphate + ADP
Cofactor
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 10 | ATP (UniProtKB | ChEBI) | ||||
Sequence: K | ||||||
Binding site | 58 | ATP (UniProtKB | ChEBI) | ||||
Sequence: F | ||||||
Binding site | 86 | ATP (UniProtKB | ChEBI) | ||||
Sequence: R | ||||||
Binding site | 92 | ATP (UniProtKB | ChEBI) | ||||
Sequence: T | ||||||
Binding site | 104 | ATP (UniProtKB | ChEBI) | ||||
Sequence: R | ||||||
Binding site | 114 | ATP (UniProtKB | ChEBI) | ||||
Sequence: N | ||||||
Active site | 117 | Pros-phosphohistidine intermediate | ||||
Sequence: H |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | ATP binding | |
Molecular Function | metal ion binding | |
Molecular Function | nucleoside diphosphate kinase activity | |
Biological Process | CTP biosynthetic process | |
Biological Process | GTP biosynthetic process | |
Biological Process | phosphorylation | |
Biological Process | UTP biosynthetic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameNucleoside diphosphate kinase
- EC number
- Short namesNDK ; NDP kinase
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Actinomycetota > Actinomycetes > Mycobacteriales > Mycobacteriaceae > Mycolicibacterium
Accessions
- Primary accessionO85501
- Secondary accessions
Proteomes
Subcellular Location
Phenotypes & Variants
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 117 | Almost loss of activity. 5-fold decrease in autophosphorylation. | ||||
Sequence: H → Q |
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000137009 | 1-139 | Nucleoside diphosphate kinase | |||
Sequence: MTERTLVLIKPDGVKRQLVGEILSRIERKGLTLAALELKNVSDDLARQHYAEHADKPFFGSLLEFITSGPLVAAIVEGPRAVAAFRQIAGGTDPVEKAVPGTIRGDFALITQDNLVHGSDSPESAAREIALWFPGEATA |
Keywords
- PTM
Proteomic databases
Interaction
Structure
Sequence
- Sequence statusComplete
- Length139
- Mass (Da)14,916
- Last updated1998-11-01 v1
- Checksum163811D0B2302030
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AF069544 EMBL· GenBank· DDBJ | AAC23864.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CP000480 EMBL· GenBank· DDBJ | ABK70634.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CP001663 EMBL· GenBank· DDBJ | AFP40964.1 EMBL· GenBank· DDBJ | Genomic DNA |