O85071 · LEU3_BUCDN
- Protein3-isopropylmalate dehydrogenase
- GeneleuB
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids363 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Catalyzes the oxidation of 3-carboxy-2-hydroxy-4-methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2-oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate.
Catalytic activity
- (2R,3S)-3-isopropylmalate + NAD+ = 4-methyl-2-oxopentanoate + CO2 + NADH
Cofactor
Mn2+ (UniProtKB | Rhea| CHEBI:29035 )
Note: Binds 1 Mg2+ or Mn2+ ion per subunit.
Pathway
Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 3/4.
Features
Showing features for binding site, site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 78-91 | NAD+ (UniProtKB | ChEBI) | ||||
Sequence: GKKWDTLPINERPE | ||||||
Binding site | 99 | substrate | ||||
Sequence: R | ||||||
Binding site | 109 | substrate | ||||
Sequence: R | ||||||
Binding site | 138 | substrate | ||||
Sequence: R | ||||||
Site | 145 | Important for catalysis | ||||
Sequence: Y | ||||||
Site | 195 | Important for catalysis | ||||
Sequence: K | ||||||
Binding site | 227 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 227 | substrate | ||||
Sequence: D | ||||||
Binding site | 251 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 255 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 285-297 | NAD+ (UniProtKB | ChEBI) | ||||
Sequence: GSAPDIQGKNIAN |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Molecular Function | 3-isopropylmalate dehydrogenase activity | |
Molecular Function | magnesium ion binding | |
Molecular Function | NAD binding | |
Biological Process | L-leucine biosynthetic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended name3-isopropylmalate dehydrogenase
- EC number
- Alternative names
Gene names
Encoded on
- Plasmid pLeu-Dn (pBDn1)
Organism names
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Enterobacterales > Erwiniaceae > Buchnera
Accessions
- Primary accessionO85071
Subcellular Location
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000083657 | 1-363 | 3-isopropylmalate dehydrogenase | |||
Sequence: MHKQYHIAVLPGDGIGPEVMQEAYKILQVLREHFSLFIKTKEFDIGGIAIDNHGIALPKKTLIGCENSDAILLGSIGGKKWDTLPINERPERASLLPLRKHFNFFCNLRPSNLYKELNFLSPLRNDIVKHGFDILCVRELTGGIYFGKPRGRVTKKKLMYAFDTEIYYKFEIVRIAHLAFKLARSRKHKLCSIDKANVLESSILWREVVEEVSKEYPDVILSHLYIDNVCMQIIKDPNQFDVLLCSNLFGDIISDECAMITGSIGMLPSASLNEKNFGLYEPAGGSAPDIQGKNIANPIAQILSLSMLIRYSMNLNKIANKIDNAVINVLKKGYKTMDISKDQNYLKTNEMGDVIADFLKRDK |
Interaction
Subunit
Homodimer.
Structure
Sequence
- Sequence statusComplete
- Length363
- Mass (Da)41,056
- Last updated1998-11-01 v1
- ChecksumC4B5F8B3E4F00735
Keywords
- Technical term