O85071 · LEU3_BUCDN

Function

function

Catalyzes the oxidation of 3-carboxy-2-hydroxy-4-methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2-oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate.

Catalytic activity

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Mn2+ (UniProtKB | Rhea| CHEBI:29035 )

Note: Binds 1 Mg2+ or Mn2+ ion per subunit.

Pathway

Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 3/4.

Features

Showing features for binding site, site.

TypeIDPosition(s)Description
Binding site78-91NAD+ (UniProtKB | ChEBI)
Binding site99substrate
Binding site109substrate
Binding site138substrate
Site145Important for catalysis
Site195Important for catalysis
Binding site227Mg2+ (UniProtKB | ChEBI)
Binding site227substrate
Binding site251Mg2+ (UniProtKB | ChEBI)
Binding site255Mg2+ (UniProtKB | ChEBI)
Binding site285-297NAD+ (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytosol
Molecular Function3-isopropylmalate dehydrogenase activity
Molecular Functionmagnesium ion binding
Molecular FunctionNAD binding
Biological ProcessL-leucine biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    3-isopropylmalate dehydrogenase
  • EC number
  • Alternative names
    • 3-IPM-DH
    • Beta-IPM dehydrogenase
      (IMDH
      )

Gene names

    • Name
      leuB

Encoded on

  • Plasmid pLeu-Dn (pBDn1)

Organism names

Accessions

  • Primary accession
    O85071

Subcellular Location

Keywords

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00000836571-3633-isopropylmalate dehydrogenase

Interaction

Subunit

Homodimer.

Structure

Family & Domains

Sequence similarities

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    363
  • Mass (Da)
    41,056
  • Last updated
    1998-11-01 v1
  • Checksum
    C4B5F8B3E4F00735
MHKQYHIAVLPGDGIGPEVMQEAYKILQVLREHFSLFIKTKEFDIGGIAIDNHGIALPKKTLIGCENSDAILLGSIGGKKWDTLPINERPERASLLPLRKHFNFFCNLRPSNLYKELNFLSPLRNDIVKHGFDILCVRELTGGIYFGKPRGRVTKKKLMYAFDTEIYYKFEIVRIAHLAFKLARSRKHKLCSIDKANVLESSILWREVVEEVSKEYPDVILSHLYIDNVCMQIIKDPNQFDVLLCSNLFGDIISDECAMITGSIGMLPSASLNEKNFGLYEPAGGSAPDIQGKNIANPIAQILSLSMLIRYSMNLNKIANKIDNAVINVLKKGYKTMDISKDQNYLKTNEMGDVIADFLKRDK

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AF041837
EMBL· GenBank· DDBJ
AAD12601.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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