O80931 · AS1_ARATH
- ProteinTranscription factor AS1
- GeneAS1
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids367 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Transcription factor required for normal cell differentiation. Positively regulates LATERAL ORGAN BOUNDARIES (LOB) within the shoot apex, and the class III HD-ZIP genes REV, PHB, and PHV. Interacts directly with ASYMMETRIC LEAVES 2 (LBD6/AS2) to repress the knox homeobox genes BP/KNAT1, KNAT2, and KNAT6 and the abaxial determinants ARF3/ETT, KAN2 and YAB5. May act in parallel with the RDR6-SGS3-AGO7 pathway, an endogenous RNA silencing pathway, to regulate the leaf morphogenesis (PubMed:11076771, PubMed:11140682, PubMed:11882937, PubMed:12750468, PubMed:16006579, PubMed:16699177, PubMed:17395603, PubMed:17559509, PubMed:23271976).
Binds directly to KNAT1, KNAT2, and KNATM chromatin, regulating leaf development (PubMed:23271976).
LBD6 is required for this binding (PubMed:23271976).
Positive regulator of flowering that binds to the promoter of FT (PubMed:21950734).
Regulates FT expression by forming a functional complex with CO (PubMed:21950734).
Involved in leaf polarity establishment by functioning cooperatively with NUCL1 to repress abaxial genes ARF3, ARF4, KAN1, KAN2, YAB1 and YAB5, and the knox homeobox genes KNAT1, KNAT2, KNAT6, and STM to promote adaxial development in leaf primordia at shoot apical meristems at high temperatures (PubMed:27334696).
Binds directly to KNAT1, KNAT2, and KNATM chromatin, regulating leaf development (PubMed:23271976).
LBD6 is required for this binding (PubMed:23271976).
Positive regulator of flowering that binds to the promoter of FT (PubMed:21950734).
Regulates FT expression by forming a functional complex with CO (PubMed:21950734).
Involved in leaf polarity establishment by functioning cooperatively with NUCL1 to repress abaxial genes ARF3, ARF4, KAN1, KAN2, YAB1 and YAB5, and the knox homeobox genes KNAT1, KNAT2, KNAT6, and STM to promote adaxial development in leaf primordia at shoot apical meristems at high temperatures (PubMed:27334696).
Features
Showing features for dna binding.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
DNA binding | 27-53 | H-T-H motif | ||||
Sequence: WHLVSERMNKPLNRDAKSCLERWKNYL | ||||||
DNA binding | 81-104 | H-T-H motif | ||||
Sequence: WKKIAAEVPGRTAKRLGKWWEVFK |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | condensed chromosome | |
Cellular Component | nucleolus | |
Cellular Component | nucleus | |
Molecular Function | DNA binding | |
Molecular Function | DNA-binding transcription factor activity | |
Molecular Function | identical protein binding | |
Molecular Function | protein homodimerization activity | |
Molecular Function | transcription cis-regulatory region binding | |
Biological Process | asymmetric cell division | |
Biological Process | defense response to bacterium | |
Biological Process | defense response to fungus | |
Biological Process | flower development | |
Biological Process | leaf formation | |
Biological Process | leaf morphogenesis | |
Biological Process | negative regulation of DNA-templated transcription | |
Biological Process | polarity specification of adaxial/abaxial axis | |
Biological Process | proximal/distal axis specification | |
Biological Process | regulation of innate immune response | |
Biological Process | response to virus |
Keywords
- Molecular function
- Biological process
Names & Taxonomy
Protein names
- Recommended nameTranscription factor AS1
- Alternative names
Gene names
Organism names
- Strains
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis
Accessions
- Primary accessionO80931
- Secondary accessions
Proteomes
Organism-specific databases
Genome annotation databases
Subcellular Location
Phenotypes & Variants
Disruption phenotype
Plants with double mutations in this protein and in RH10 or AS2 protein have abaxialized filamentous and trumpet-like leaves at high temperatures. In double mutants, shapes of epidermal cells of the filamentous leaves are simple and rectangular, similar to those of a petiole, but different from those of flat leaves of wild-type plants.
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 11 variants from UniProt as well as other sources including ClinVar and dbSNP.
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000197073 | 1-367 | Transcription factor AS1 | |||
Sequence: MKERQRWSGEEDALLRAYVRQFGPREWHLVSERMNKPLNRDAKSCLERWKNYLKPGIKKGSLTEEEQRLVIRLQEKHGNKWKKIAAEVPGRTAKRLGKWWEVFKEKQQREEKESNKRVEPIDESKYDRILESFAEKLVKERSNVVPAAAAAATVVMANSNGGFLHSEQQVQPPNPVIPPWLATSNNGNNVVARPPSVTLTLSPSTVAAAAPQPPIPWLQQQQPERAENGPGGLVLGSMMPSCSGSSESVFLSELVECCRELEEGHRAWADHKKEAAWRLRRLELQLESEKTCRQREKMEEIEAKMKALREEQKNAMEKIEGEYREQLVGLRRDAEAKDQKLADQWTSRHIRLTKFLEQQMGCRLDRP |
Proteomic databases
Expression
Tissue specificity
Expressed in roots, stems, leaves, flowers, siliques and in lateral organ promordia (PubMed:17559509).
Found in the inner domain between the adaxial and abaxial domains of leaves (PubMed:17559509).
Expressed in the phloem tissues of leaves, cotyledons, hypocotyls, and roots (PubMed:21950734).
Found in the inner domain between the adaxial and abaxial domains of leaves (PubMed:17559509).
Expressed in the phloem tissues of leaves, cotyledons, hypocotyls, and roots (PubMed:21950734).
Induction
Circadian-regulation with an afternoon peak in long days and with a broad night peak in short days (PubMed:21950734).
Expression of AS1 in stem cells of the shoot apical meristem is prevented by SHOOT MERISTEMLESS (STM). Expression is activated by GTE6 during leaf morphogenesis (PubMed:16166385).
Expression of AS1 in stem cells of the shoot apical meristem is prevented by SHOOT MERISTEMLESS (STM). Expression is activated by GTE6 during leaf morphogenesis (PubMed:16166385).
Developmental stage
Preferential expression in young and immature plant tissues. In embryos, expressed from the late globular stage onwards. After germination, detected in leaf founder cells and on flowering, in primordia of all floral organs.
Gene expression databases
Interaction
Subunit
Homodimer (PubMed:12750468, PubMed:23271976).
Heterodimer with LBD6 (PubMed:23271976).
Interacts with LBD6/AS2, HIRA, a probable histone chaperone, and RIK, a predicted RNA binding protein (PubMed:12874130, PubMed:16243907).
Part of the AS1 repressor complex composed of AS1, LBD6/AS2 and HDA6 (PubMed:23271976).
Interacts with CO (PubMed:21950734).
Heterodimer with LBD6 (PubMed:23271976).
Interacts with LBD6/AS2, HIRA, a probable histone chaperone, and RIK, a predicted RNA binding protein (PubMed:12874130, PubMed:16243907).
Part of the AS1 repressor complex composed of AS1, LBD6/AS2 and HDA6 (PubMed:23271976).
Interacts with CO (PubMed:21950734).
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | Intact | |
---|---|---|---|---|---|
BINARY | O80931 | AHG1 Q9FLI3 | 3 | EBI-763232, EBI-2363348 | |
BINARY | O80931 | ARR14 Q8L9Y3 | 4 | EBI-763232, EBI-1100737 | |
BINARY | O80931 | AS1 O80931 | 4 | EBI-763232, EBI-763232 | |
BINARY | O80931 | AS2 O04479 | 6 | EBI-763232, EBI-4443304 | |
BINARY | O80931 | AXX17_At1g45300 A0A178W725 | 3 | EBI-763232, EBI-25515760 | |
BINARY | O80931 | GI Q9SQI2 | 3 | EBI-763232, EBI-446380 | |
BINARY | O80931 | MPK19 Q9LUC3 | 3 | EBI-763232, EBI-25512843 | |
BINARY | O80931 | MPK20 Q9SJG9 | 3 | EBI-763232, EBI-2358896 | |
BINARY | O80931 | MYB73 O23160 | 3 | EBI-763232, EBI-25506855 | |
BINARY | O80931 | NIA2 P11035 | 4 | EBI-763232, EBI-4451150 | |
BINARY | O80931 | PP2AA1 Q38845 | 3 | EBI-763232, EBI-1645478 | |
BINARY | O80931 | PRDA1 Q1H5E9 | 3 | EBI-763232, EBI-1998055 | |
BINARY | O80931 | RCD1 Q8RY59 | 2 | EBI-763232, EBI-2118043 |
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for domain, coiled coil.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 1-53 | HTH myb-type 1 | ||||
Sequence: MKERQRWSGEEDALLRAYVRQFGPREWHLVSERMNKPLNRDAKSCLERWKNYL | ||||||
Domain | 54-108 | HTH myb-type 2 | ||||
Sequence: KPGIKKGSLTEEEQRLVIRLQEKHGNKWKKIAAEVPGRTAKRLGKWWEVFKEKQQ | ||||||
Coiled coil | 260-344 | |||||
Sequence: ELEEGHRAWADHKKEAAWRLRRLELQLESEKTCRQREKMEEIEAKMKALREEQKNAMEKIEGEYREQLVGLRRDAEAKDQKLADQ |
Domain
A C-terminal domain (252-356) is required for dimerization.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length367
- Mass (Da)42,243
- Last updated1998-11-01 v1
- Checksum24D9085193E6088B
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AF175996 EMBL· GenBank· DDBJ | AAD53101.1 EMBL· GenBank· DDBJ | mRNA | ||
AY519578 EMBL· GenBank· DDBJ | AAS10048.1 EMBL· GenBank· DDBJ | mRNA | ||
AC004684 EMBL· GenBank· DDBJ | AAC23633.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CP002685 EMBL· GenBank· DDBJ | AEC09428.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BT026027 EMBL· GenBank· DDBJ | ABG48383.1 EMBL· GenBank· DDBJ | mRNA |