O80474 · MES4_ARATH
- ProteinMethylesterase 4
- GeneMES4
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids263 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
Methylesterase shown to have carboxylesterase activity and methyl salicylate (MeSA) esterase activity in vitro.
Catalytic activity
- H2O + methyl salicylate = H+ + methanol + salicylateThis reaction proceeds in the forward direction.
Activity regulation
Esterase activity is down-regulated by salicylic acid (SA).
Kinetics
Vmax | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|
16.07 nmol/min/ug | with methyl salicylate (MeSA) as substrate |
Pathway
Plant hormone biosynthesis.
Features
Showing features for active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Active site | 84 | Acyl-ester intermediate | ||||
Sequence: S | ||||||
Active site | 213 | Charge relay system | ||||
Sequence: D | ||||||
Active site | 241 | Charge relay system | ||||
Sequence: H |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | vacuole | |
Molecular Function | hydrolase activity, acting on ester bonds | |
Molecular Function | methyl salicylate esterase activity | |
Biological Process | salicylic acid metabolic process |
Keywords
- Molecular function
Enzyme and pathway databases
Protein family/group databases
Names & Taxonomy
Protein names
- Recommended nameMethylesterase 4
- EC number
- Short namesAtMES4
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis
Accessions
- Primary accessionO80474
Proteomes
Organism-specific databases
Genome annotation databases
Subcellular Location
UniProt Annotation
GO Annotation
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000418179 | 1-263 | Methylesterase 4 | |||
Sequence: MEKNNKKRFVLVHGLCHGAWCWYKVKTHLEAVGHCVTAVDLAASGINMTRLEEIQTLKDYCKPLLELLNSLGSDDDKVILVAHSMGGIPAALASDIFPSKIATIVFLTAFMPDTRNLPAYVYQKLIRSVPQEGWLDTVFGTYGKHECPLEFALFGPKFMAKNLYQLSPVQDLELAKMLVRVNPIITNNLAGTRSFSEEGYGTVTRIYIVCGEDMAVPEDYQWWMIKNFPPKEVMEIKCADHMAMFSKPHKLCALLVEIACKYA |
Proteomic databases
Expression
Gene expression databases
Structure
Family & Domains
Sequence similarities
Belongs to the AB hydrolase superfamily. Methylesterase family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
This entry describes 1 isoforms produced by Alternative splicing. A number of isoforms are produced. According to EST sequences.
O80474-1
This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
- Name1
- Length263
- Mass (Da)29,582
- Last updated1998-11-01 v1
- ChecksumE8EAE4A37C5C3800
Computationally mapped potential isoform sequences
There is 1 potential isoform mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
F4IMK6 | F4IMK6_ARATH | MES4 | 203 |
Keywords
- Coding sequence diversity
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AC003040 EMBL· GenBank· DDBJ | AAC23771.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CP002685 EMBL· GenBank· DDBJ | AEC07468.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BT010429 EMBL· GenBank· DDBJ | AAQ62430.1 EMBL· GenBank· DDBJ | mRNA | ||
AK175772 EMBL· GenBank· DDBJ | BAD43535.1 EMBL· GenBank· DDBJ | mRNA |