O80358 · FPG_ARATH

Function

function

Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as a DNA glycosylase that recognizes and removes damaged bases. Can process efficiently 4,6-diamino-5-formamidopyrimidine (FapyA), 2,6-diamino-4- hydroxy-5-formamidopyrimidine (FapyG) and the further oxidation products of 8-oxoguanine (8-oxoG), such as guanidinohydantoin and spiroiminodihydantoin. Has marginal activity towards 8-oxoG. Has AP (apurinic/apyrimidinic) lyase activity. Cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base with both 3'- and 5'-phosphates.

Catalytic activity

Features

Showing features for active site, binding site.

TypeIDPosition(s)Description
Active site2Schiff-base intermediate with DNA
Active site3Proton donor
Active site60Proton donor; for beta-elimination activity
Binding site107DNA (UniProtKB | ChEBI)
Binding site126DNA (UniProtKB | ChEBI)
Binding site167DNA (UniProtKB | ChEBI)
Binding site186DNA (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentnucleus
Molecular Functionclass I DNA-(apurinic or apyrimidinic site) endonuclease activity
Molecular Functiondamaged DNA binding
Molecular FunctionDNA N-glycosylase activity
Molecular Functionoxidized purine nucleobase lesion DNA N-glycosylase activity
Molecular Functionzinc ion binding
Biological Processbase-excision repair
Biological ProcessDNA repair
Biological Processresponse to oxidative stress

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Formamidopyrimidine-DNA glycosylase
  • EC number
  • Short names
    Fapy-DNA glycosylase
  • Alternative names
    • DNA-(apurinic or apyrimidinic site) lyase FPG1
    • Formamidopyrimidine-DNA glycosylase 1 (AtFPG-1)
    • Formamidopyrimidine-DNA glycosylase 2 (AtFPG-2)
    • Protein MutM homolog 1 (AtMMH-1)
    • Protein MutM homolog 2 (AtMMH-2)

Gene names

    • Name
      FPG1
    • Synonyms
      FPG2
    • ORF names
      F6D8.28
    • Ordered locus names
      At1g52500

Organism names

  • Taxonomic identifier
  • Strains
    • cv. Columbia
    • cv. Landsberg erecta
  • Taxonomic lineage
    Eukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis

Accessions

  • Primary accession
    O80358
  • Secondary accessions
    • O80359
    • Q9SBB4

Proteomes

Organism-specific databases

Genome annotation databases

Subcellular Location

Keywords

Phenotypes & Variants

Disruption phenotype

No visible phenotype under normal growth conditions or UV-A irradiation stress.

Variants

We now provide the "Disease & Variants" viewer in its own tab.

The viewer provides 38 variants from UniProt as well as other sources including ClinVar and dbSNP.

Go to variant viewer

PTM/Processing

Features

Showing features for initiator methionine, chain.

TypeIDPosition(s)Description
Initiator methionine1Removed
ChainPRO_00004212612-390Formamidopyrimidine-DNA glycosylase

Proteomic databases

PTM databases

Expression

Tissue specificity

Expressed in leaves (at protein levels).

Gene expression databases

Interaction

Subunit

Monomer.

Protein-protein interaction databases

Family & Domains

Features

Showing features for compositional bias, region.

TypeIDPosition(s)Description
Compositional bias283-301Basic and acidic residues
Region283-390Disordered
Compositional bias354-382Basic and acidic residues

Sequence similarities

Belongs to the FPG family.

Phylogenomic databases

Family and domain databases

Sequence & Isoform

Align isoforms (2)
  • Sequence status
    Complete

This entry describes 2 isoforms produced by Alternative splicing.

O80358-1

This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

  • Length
    390
  • Mass (Da)
    43,194
  • Last updated
    1998-11-01 v1
  • Checksum
    F6B5E0DAFD6FD010
MPELPEVEAARRAIEENCLGKKIKRVIIADDNKVIHGISPSDFQTSILGKTIISARRKGKNLWLELDSPPFPSFQFGMAGAIYIKGVAVTKYKRSAVKDSEEWPSKYSKFFVELDDGLELSFTDKRRFAKVRLLANPTSVSPISELGPDALLEPMTVDEFAESLAKKKITIKPLLLDQGYISGIGNWIADEVLYQARIHPLQTASSLSKEQCEALHTSIKEVIEKAVEVDADSSQFPSYWIFHNREKKPGKAFVDGKKIDFITAGGRTTAYVPELQKLYGKDAEKAAKVRPAKRGVKPKEDDGDGEEDEQETEKEDESAKSKKGQKPRGGRGKKPASKTKTEESDDDGDDSEAEEEVVKPKGRGTKPAIKRKSEEKATSQAGKKPKGRKS

O80358-2

  • Name
    2
  • See also
    sequence in UniParc or sequence clusters in UniRef
  • Differences from canonical
    • 224-274: EKAVEVDADSSQFPSYWIFHNREKKPGKAFVDGKKIDFITAGGRTTAYVPE → QHAVQVNADSKEFPVEWLFHFRWGKKAGKVNGKLSHHLSINLMKQNLGFCR
    • 275-390: Missing

Computationally mapped potential isoform sequences

There are 2 potential isoforms mapped to this entry

View all
EntryEntry nameGene nameLength
A0A1P8AVR7A0A1P8AVR7_ARATHMMH-1288
A0A178VZV9A0A178VZV9_ARATHMMH-1224

Features

Showing features for alternative sequence, sequence conflict, compositional bias.

TypeIDPosition(s)Description
Alternative sequenceVSP_045377224-274in isoform 2
Sequence conflict239in Ref. 2; AAC97952
Alternative sequenceVSP_045378275-390in isoform 2
Compositional bias283-301Basic and acidic residues
Compositional bias354-382Basic and acidic residues

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AB010690
EMBL· GenBank· DDBJ
BAA32702.1
EMBL· GenBank· DDBJ
Genomic DNA
AB010690
EMBL· GenBank· DDBJ
BAA32703.1
EMBL· GenBank· DDBJ
Genomic DNA
AF099970
EMBL· GenBank· DDBJ
AAC97952.1
EMBL· GenBank· DDBJ
mRNA
AF099971
EMBL· GenBank· DDBJ
AAC97953.1
EMBL· GenBank· DDBJ
mRNA
AC008016
EMBL· GenBank· DDBJ
AAD55612.1
EMBL· GenBank· DDBJ
Genomic DNA
AC008016
EMBL· GenBank· DDBJ
AAD55613.1
EMBL· GenBank· DDBJ
Genomic DNA
CP002684
EMBL· GenBank· DDBJ
AEE32814.1
EMBL· GenBank· DDBJ
Genomic DNA
CP002684
EMBL· GenBank· DDBJ
AEE32815.1
EMBL· GenBank· DDBJ
Genomic DNA
CP002684
EMBL· GenBank· DDBJ
ANM60729.1
EMBL· GenBank· DDBJ
Genomic DNA
BT043496
EMBL· GenBank· DDBJ
ACG58696.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

Disclaimer

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