O77264 · RTCA_DROME
- ProteinRNA 3'-terminal phosphate cyclase
- GeneRtca
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids361 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
Catalyzes the conversion of 3'-phosphate to a 2',3'-cyclic phosphodiester at the end of RNA (By similarity).
The mechanism of action of the enzyme occurs in 3 steps: (A) adenylation of the enzyme by ATP; (B) transfer of adenylate to an RNA-N3'P to produce RNA-N3'PP5'A; (C) and attack of the adjacent 2'-hydroxyl on the 3'-phosphorus in the diester linkage to produce the cyclic end product (By similarity).
Likely functions in some aspects of cellular RNA processing (By similarity).
Function plays an important role in a RNA repair and splicing pathway which controls axon regeneration in response to peripheral (PNS) and central nervous system (CNS) injury (PubMed:25961792, PubMed:31919191).
In response to axotomy, negatively regulates splicing of Xbp1 which in turn activates downstream effectors which inhibit axon regeneration, including down-regulating the microtubule regulators ringer and futsch (PubMed:25961792, PubMed:31919191).
The mechanism of action of the enzyme occurs in 3 steps: (A) adenylation of the enzyme by ATP; (B) transfer of adenylate to an RNA-N3'P to produce RNA-N3'PP5'A; (C) and attack of the adjacent 2'-hydroxyl on the 3'-phosphorus in the diester linkage to produce the cyclic end product (By similarity).
Likely functions in some aspects of cellular RNA processing (By similarity).
Function plays an important role in a RNA repair and splicing pathway which controls axon regeneration in response to peripheral (PNS) and central nervous system (CNS) injury (PubMed:25961792, PubMed:31919191).
In response to axotomy, negatively regulates splicing of Xbp1 which in turn activates downstream effectors which inhibit axon regeneration, including down-regulating the microtubule regulators ringer and futsch (PubMed:25961792, PubMed:31919191).
Catalytic activity
- a 3'-end 3'-phospho-ribonucleotide-RNA + ATP = a 3'-end 2',3'-cyclophospho-ribonucleotide-RNA + AMP + diphosphate
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 105 | ATP (UniProtKB | ChEBI) | ||||
Sequence: Q | ||||||
Binding site | 132 | ATP (UniProtKB | ChEBI) | ||||
Sequence: P | ||||||
Binding site | 295 | ATP (UniProtKB | ChEBI) | ||||
Sequence: Y | ||||||
Binding site | 298 | ATP (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 299 | ATP (UniProtKB | ChEBI) | ||||
Sequence: Q | ||||||
Active site | 321 | Tele-AMP-histidine intermediate | ||||
Sequence: H | ||||||
Binding site | 321 | ATP (UniProtKB | ChEBI) | ||||
Sequence: H |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | nucleoplasm | |
Cellular Component | nucleus | |
Molecular Function | ATP binding | |
Molecular Function | RNA-3'-phosphate cyclase activity | |
Biological Process | negative regulation of axon regeneration | |
Biological Process | RNA processing |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameRNA 3'-terminal phosphate cyclase
- EC number
- Short namesRNA cyclase ; RNA-3'-phosphate cyclase
Gene names
Organism names
- Strains
- Taxonomic lineageEukaryota > Metazoa > Ecdysozoa > Arthropoda > Hexapoda > Insecta > Pterygota > Neoptera > Endopterygota > Diptera > Brachycera > Muscomorpha > Ephydroidea > Drosophilidae > Drosophila > Sophophora
Accessions
- Primary accessionO77264
Proteomes
Organism-specific databases
Subcellular Location
Phenotypes & Variants
Disruption phenotype
Severed axons of RNAi-mediated knockdown class III dendritic arborization (da) neurons, display increased regeneration.
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000156412 | 1-361 | RNA 3'-terminal phosphate cyclase | |||
Sequence: MDAEKMLEIDGSYLEGGGQALRNALSLSCILGKPVRVVKIRASRPSPGLSHQHLHGLNLLRDITNADVVGNYLLSSTVEFTPRTILDNTYRVETHTAASITLIYQMALPVLLFAGRPSRLIVSGGTNVDFAPPVEYMQEVLLPNLKHFGVSFDLKVQRYGFYPRGQGRCQLDVQPVTKLNSGKLVAFGRIKSVSGVAYCAGRLPVNIAIDMQQTAQREIHRLWPSQQCSIEPIKHSRQKAFHNGAGILMTVNTTSDVVLGASALGKKRIDGHVLGSEASCQLGDYMRKQVCVDDYMQDQLIIYMALAVGRSTMRTGKLTNHTRTAINVAEQMTGVKFDVAMEPGGQMLVSCKGLGHVNKLI |
Proteomic databases
Expression
Developmental stage
In larvae, detected in various types of multidendritic neurons including class IV and class III dendritic arborization (da) neurons (at protein level) (PubMed:25961792).
In larvae, expressed in class IV da neurons, and tissues in the peripheral nervous system (PNS) and the ventral nerve cord (VNC) (PubMed:25961792).
In larvae, expressed in class IV da neurons, and tissues in the peripheral nervous system (PNS) and the ventral nerve cord (VNC) (PubMed:25961792).
Gene expression databases
Structure
Sequence
- Sequence statusComplete
- Length361
- Mass (Da)39,503
- Last updated1998-11-01 v1
- ChecksumCDB65A453BB8CF39
Computationally mapped potential isoform sequences
There is 1 potential isoform mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
X2JI07 | X2JI07_DROME | Rtca | 361 |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AE014298 EMBL· GenBank· DDBJ | AAF45719.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AL031765 EMBL· GenBank· DDBJ | CAA21129.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AY052112 EMBL· GenBank· DDBJ | AAK93536.1 EMBL· GenBank· DDBJ | mRNA |