O77203 · PIAA_DICDI
- ProteinProtein pianissimo A
- GenepiaA
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids1148 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Regulates cell growth, chemotaxis, signal relay and the actin cytoskeleton. Required for chemoattractant receptor and G protein-mediated activation of the 12 transmembrane domain adenylyl cyclase. Functions as a part of protein complex TORC2. TORC2, is presumed to be indirectly negatively modulated by rapamycin and regulates actin polarization. TORC2, but not TORC1, negatively regulates phagocytosis. This protein and dagA protein CRAC, a cytosolic regulator, are both essential for activation of the enzyme adenylyl cyclase. This protein and CRAC do not function redundantly. Both proteins are integral components of the adenylyl cyclase activation pathway.
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Keywords
- Molecular function
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameProtein pianissimo A
- Alternative names
Gene names
Organism names
- Strains
- Taxonomic lineageEukaryota > Amoebozoa > Evosea > Eumycetozoa > Dictyostelia > Dictyosteliales > Dictyosteliaceae > Dictyostelium
Accessions
- Primary accessionO77203
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
Phenotypes & Variants
Disruption phenotype
Cells are unable to aggregate on bacterial lawns and show impaired chemotaxis, rounded morphology and lack polarity. Phosphorylations of endogenous pkbA/PKB substrates greatly reduced including loss of phosphorylation at 'Thr-470' in endogenous pkgB. In null cells neither chemoattractant stimulation of intact cells nor guanosine gamma thio-phosphate treatment of lysates activates the enzyme adenylyl cyclase. Constitutive expression of piaA reverses these defects. dagA and piaA double mutants require both proteins for reconstitution and activation of adenylyl cyclase. Null cells can respond to exogenous signals by expression of developmental genes necessary for spore formation, although the efficiency of the process is reduced. Null cells possess the machinery to respond to cAMP signals. However, they are unable to aggregate in pure populations suggesting that the defect may be in the production of the cAMP signals.
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 917 | Temperature-sensitive phenotype and defective G protein-linked adenylyl cyclase activation. | ||||
Sequence: G → D |
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000377480 | 1-1148 | Protein pianissimo A | |||
Sequence: MTSSDSSVNTTSSSFGNISISSPNHSSSTPPLNNGNGNNVSASETELKKHVLYSLQCLDEKTLTLKVKLDHLNKLVELKKSIPDLNKLGISPTQLYKSVRPFIALPPKTIRTAGLRVMRYYLSNSNNVKELLDLKVQYFITRSLERDKHSEPERIQALKIIRTIMEIDCSLMPHCFVKGLVSIAENQEDNFCRVCLECLTEISIRNPQISSHCGGIRTVFDAVLDPFYQGIQESLLICILYLLSDKDTRIYIRPKSDLEIILAPLTNSFNIGVKLKGASKEKEKEKEKEDEVAMKKWTASSKAVLTLIKSWIGIISLNSDDQGLKSVVDTLRMPQIELQEKALDSIFEIFRVQLPKSIQETFGPQKATQTFNFGSETLQDLPSRTRSLRHNLLNNYLSVLLIAFIDNGLIEGLVYLGNYVANRDGMSEQEKECSKNISLKSTVLLAELLHMSNALLPPSQCAKLQTLPSLVNSAISFRLDPRLRSSSNTMVTNLHSYSHNKSSTTLMDSTLAIGLTGANKWRRIKGQDRRLDKVDDVKMKMEWHMDDNQFQQKIKDTQVLVTKDYQKWSWELMFELLEGPLNNPQHLSNTLKTKFIKRILSFLRPNKKLFSTMAWTTENLKYVRTACVALEVLISHEIGFDFLKDNKTIIQIADMLKVELDYNIKPPPSSSSSSENKKDNVRLLNPEKVLKTMSREYFTMLGTLSSNLLGLEILARNNIFDYIKPLAELPGRDDLSHLIMTSLDYNVNGASRTILQKILTSSSRVVRYLATKYLRFLLRSGVQDFSNWGVELLVQQLNDVDAKVSALSLNVLDEACDDPSCLEVLIDLKPNLLKLGKPGKSLLLRFLSSPKGLENLLQNNGFVEQEEQLWITSENATYVNAIESAVSESLSPSVWRFKEAPDGSSTSGVYLPPHFFGELAKTEKGCQLIRKSNNYQRFLKIIQDPTAKQLDKRASLIAIGHIGSSVDGYSFVKESDTIKLLIGIAEKSQCLALRSTCFYALGMISCIEEAQPIFNSFGWESPSDLNSRILLPKDLKNSTFLSVPQYQYQGSWADHSFETLPSNHFSDPIKNEIISFVGNLSSHITAEGASKNLKRLKIKYPDHFATSEILNAVFILLNTFKYRLGARRFIYDLFDVAIFSSDPYHDLN |
Proteomic databases
Expression
Developmental stage
Expression peaks between 2.5 and 5 hours of development.
Structure
Family & Domains
Features
Showing features for region, domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 1-41 | Disordered | ||||
Sequence: MTSSDSSVNTTSSSFGNISISSPNHSSSTPPLNNGNGNNVS | ||||||
Domain | 803-914 | N-terminal Ras-GEF | ||||
Sequence: KVSALSLNVLDEACDDPSCLEVLIDLKPNLLKLGKPGKSLLLRFLSSPKGLENLLQNNGFVEQEEQLWITSENATYVNAIESAVSESLSPSVWRFKEAPDGSSTSGVYLPPH |
Sequence similarities
Belongs to the RICTOR family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length1,148
- Mass (Da)129,529
- Last updated1998-11-01 v1
- ChecksumA417189FA3B3DBAE
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AF085194 EMBL· GenBank· DDBJ | AAC35553.1 EMBL· GenBank· DDBJ | mRNA | ||
AAFI02000020 EMBL· GenBank· DDBJ | EAL68662.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AF080675 EMBL· GenBank· DDBJ | AAC31824.1 EMBL· GenBank· DDBJ | Genomic DNA |