O75828 · CBR3_HUMAN
- ProteinCarbonyl reductase [NADPH] 3
- GeneCBR3
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids277 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Catalyzes the NADPH-dependent reduction of carbonyl compounds to their corresponding alcohols (PubMed:18493841).
Has low NADPH-dependent oxidoreductase activity. Acts on several orthoquinones, acts as well on non-quinone compounds, such as isatin or on the anticancer drug oracin (PubMed:15537833, PubMed:18493841, PubMed:19841672).
Best substrates for CBR3 is 1,2- naphthoquinone, hence could play a role in protection against cytotoxicity of exogenous quinones (PubMed:19841672).
Exerts activity toward ortho-quinones but not paraquinones. No endogenous substrate for CBR3 except isatin has been identified (PubMed:19841672).
Has low NADPH-dependent oxidoreductase activity. Acts on several orthoquinones, acts as well on non-quinone compounds, such as isatin or on the anticancer drug oracin (PubMed:15537833, PubMed:18493841, PubMed:19841672).
Best substrates for CBR3 is 1,2- naphthoquinone, hence could play a role in protection against cytotoxicity of exogenous quinones (PubMed:19841672).
Exerts activity toward ortho-quinones but not paraquinones. No endogenous substrate for CBR3 except isatin has been identified (PubMed:19841672).
Catalytic activity
- a secondary alcohol + NADP+ = a ketone + H+ + NADPHThis reaction proceeds in the backward direction.
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
25 μM | menadione | |||||
43 μM | menadione | |||||
300 μM | 4-benzoylpyridine | |||||
130 μM | 4-nitorobenzaldehyde | |||||
420 μM | 1,2-naphthoquinone | |||||
14630 μM | isatin | |||||
140 μM | oracin | |||||
90 μM | NADPH | |||||
38 μM | NADPH |
Vmax | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|
6 μmol/min/mg | with 1,2-naphthoquinone as substrate | ||||
0.1 μmol/min/mg | with oracin as substrate | ||||
15 μmol/min/mg | with isatin as substrate |
kcat is 0.55 min-1 with menadione as substrate (PubMed:18493841).
kcat is 0.70 min-1 with 4-benzoylpyridine as substrate (PubMed:18493841).
kcat is 0.59 min-1 with 4-nitorobenzaldehyde as substrate (PubMed:18493841).
kcat is 0.70 min-1 with 4-benzoylpyridine as substrate (PubMed:18493841).
kcat is 0.59 min-1 with 4-nitorobenzaldehyde as substrate (PubMed:18493841).
pH Dependence
Optimum pH is 5.5-7.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 10-34 | NADP+ (UniProtKB | ChEBI) | ||||
Sequence: VTGANRGIGLAIARELCRQFSGDVV | ||||||
Binding site | 38-42 | NADP+ (UniProtKB | ChEBI) | ||||
Sequence: RDVAR | ||||||
Binding site | 63-64 | NADP+ (UniProtKB | ChEBI) | ||||
Sequence: DI | ||||||
Binding site | 90 | NADP+ (UniProtKB | ChEBI) | ||||
Sequence: N | ||||||
Binding site | 140 | substrate | ||||
Sequence: S | ||||||
Active site | 194 | Proton acceptor | ||||
Sequence: Y | ||||||
Binding site | 194-198 | NADP+ (UniProtKB | ChEBI) | ||||
Sequence: YGVSK | ||||||
Binding site | 239 | NADP+ (UniProtKB | ChEBI) | ||||
Sequence: D |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Cellular Component | extracellular space | |
Cellular Component | nucleoplasm | |
Molecular Function | 3-keto sterol reductase activity | |
Molecular Function | carbonyl reductase (NADPH) activity | |
Molecular Function | NADPH binding | |
Molecular Function | NADPH dehydrogenase (quinone) activity | |
Biological Process | cognition | |
Biological Process | phylloquinone catabolic process | |
Biological Process | xenobiotic metabolic process |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameCarbonyl reductase [NADPH] 3
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Primates > Haplorrhini > Catarrhini > Hominidae > Homo
Accessions
- Primary accessionO75828
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
Disease & Variants
Features
Showing features for natural variant, mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Natural variant | VAR_033868 | 4 | in dbSNP:rs8133052 | |||
Sequence: C → Y | ||||||
Natural variant | VAR_033869 | 84 | in dbSNP:rs9282628 | |||
Sequence: L → V | ||||||
Natural variant | VAR_033870 | 93 | in dbSNP:rs2835285 | |||
Sequence: V → I | ||||||
Natural variant | VAR_033871 | 131 | in dbSNP:rs16993929 | |||
Sequence: P → S | ||||||
Mutagenesis | 142 | Modest increase in carbonyl reductase (NADPH) activity toward 1,2-naphthoquinone. | ||||
Sequence: Q → M | ||||||
Mutagenesis | 230 | Decreased carbonyl reductase (NADPH) activity toward 1,2-naphthoquinone and isatin. | ||||
Sequence: P → F or W | ||||||
Natural variant | VAR_033872 | 235 | in dbSNP:rs4987121 | |||
Sequence: M → L | ||||||
Mutagenesis | 236 | Significant decreased of the Km value for isatin. | ||||
Sequence: D → A | ||||||
Natural variant | VAR_033873 | 244 | increased carbonyl reductase (NADPH) activity; exhibits a 2-fold higher Vmax with menadione; higher Vmax with NADP(H); does not affect Km for menadione; dbSNP:rs1056892 | |||
Sequence: V → M |
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 514 variants from UniProt as well as other sources including ClinVar and dbSNP.
Organism-specific databases
Miscellaneous
Chemistry
Genetic variation databases
PTM/Processing
Features
Showing features for initiator methionine, modified residue, chain, modified residue (large scale data).
Type | ID | Position(s) | Source | Description | |||
---|---|---|---|---|---|---|---|
Initiator methionine | 1 | UniProt | Removed | ||||
Sequence: M | |||||||
Modified residue | 2 | UniProt | N-acetylserine | ||||
Sequence: S | |||||||
Chain | PRO_0000054608 | 2-277 | UniProt | Carbonyl reductase [NADPH] 3 | |||
Sequence: SSCSRVALVTGANRGIGLAIARELCRQFSGDVVLTARDVARGQAAVQQLQAEGLSPRFHQLDIDDLQSIRALRDFLRKEYGGLNVLVNNAAVAFKSDDPMPFDIKAEMTLKTNFFATRNMCNELLPIMKPHGRVVNISSLQCLRAFENCSEDLQERFHSETLTEGDLVDLMKKFVEDTKNEVHEREGWPNSPYGVSKLGVTVLSRILARRLDEKRKADRILVNACCPGPVKTDMDGKDSIRTVEEGAETPVYLALLPPDATEPQGQLVHDKVVQNW | |||||||
Modified residue | 30 | UniProt | Phosphoserine | ||||
Sequence: S | |||||||
Modified residue (large scale data) | 56 | PRIDE | Phosphoserine | ||||
Sequence: S | |||||||
Modified residue (large scale data) | 192 | PRIDE | Phosphoserine | ||||
Sequence: S |
Keywords
- PTM
Proteomic databases
2D gel databases
PTM databases
Expression
Tissue specificity
Detected in ovary, pancreas, intestine, colon, kidney, brain, thymus, lung, heart, liver, spleen, leukocyte, prostate and testis.
Gene expression databases
Organism-specific databases
Interaction
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | Intact | |
---|---|---|---|---|---|
BINARY | O75828 | BICD2 Q8TD16-2 | 3 | EBI-714504, EBI-11975051 | |
BINARY | O75828 | CBR1 P16152 | 4 | EBI-714504, EBI-351348 | |
BINARY | O75828 | SDCBP O00560 | 8 | EBI-714504, EBI-727004 |
Protein-protein interaction databases
Miscellaneous
Structure
Sequence
- Sequence statusComplete
- Length277
- Mass (Da)30,850
- Last updated2007-01-23 v3
- Checksum8D39D6B99CFE5389
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AB004854 EMBL· GenBank· DDBJ | BAA33500.1 EMBL· GenBank· DDBJ | mRNA | ||
AB003151 EMBL· GenBank· DDBJ | BAA34207.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AB041012 EMBL· GenBank· DDBJ | BAD74062.1 EMBL· GenBank· DDBJ | mRNA | ||
AB124847 EMBL· GenBank· DDBJ | BAE45939.1 EMBL· GenBank· DDBJ | mRNA | ||
CR541709 EMBL· GenBank· DDBJ | CAG46510.1 EMBL· GenBank· DDBJ | mRNA | ||
EF462915 EMBL· GenBank· DDBJ | ABO43035.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AP000689 EMBL· GenBank· DDBJ | BAA89425.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AP001725 EMBL· GenBank· DDBJ | BAA95547.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CH471079 EMBL· GenBank· DDBJ | EAX09747.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BC002812 EMBL· GenBank· DDBJ | AAH02812.1 EMBL· GenBank· DDBJ | mRNA |