O71188 · R1A_GLRV3
- ProteinReplicase protein 1a
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids2233 (go to sequence)
- Protein existenceInferred from homology
- Annotation score4/5
Function
Catalytic activity
- ATP + H2O = ADP + H+ + phosphate
Cofactor
Features
Showing features for active site, site, binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Active site | 311 | For leader protease activity | ||||
Sequence: C | ||||||
Active site | 357 | For leader protease activity | ||||
Sequence: H | ||||||
Site | 393-394 | Cleavage; by the leader protease | ||||
Sequence: GG | ||||||
Binding site | 1619 | Fe cation (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Binding site | 1621 | Fe cation (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 1676 | Fe cation (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Binding site | 1685 | 2-oxoglutarate (UniProtKB | ChEBI) | ||||
Sequence: R |
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Molecular Function | ATP binding | |
Molecular Function | ATP hydrolysis activity | |
Molecular Function | cysteine-type peptidase activity | |
Molecular Function | dioxygenase activity | |
Molecular Function | metal ion binding | |
Molecular Function | mRNA methyltransferase activity | |
Molecular Function | RNA binding | |
Molecular Function | RNA helicase activity | |
Biological Process | methylation | |
Biological Process | mRNA modification | |
Biological Process | proteolysis | |
Biological Process | RNA processing | |
Biological Process | viral translational frameshifting |
Keywords
- Molecular function
- Ligand
Names & Taxonomy
Protein names
- Recommended nameReplicase protein 1a
- Cleaved into 2 chains
Gene names
Organism names
- Taxonomic lineageViruses > Riboviria > Orthornavirae > Kitrinoviricota > Alsuviricetes > Martellivirales > Closteroviridae > Ampelovirus > Grapevine leafroll-associated virus 3
- Virus hosts
Accessions
- Primary accessionO71188
Proteomes
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000402532 | 1-393 | Leader protease | |||
Sequence: MDYIRPLRVFSFPHVNNTLEYVRYNKANGDVGAFLTTMKFIGNVKLSDFTPRCAAMIYIGKLTKGVKRTFVPPPVKGFARQYAVVSGSVSALRGDGKKVLMEARTSTSATSDVSDFDVVFEAVSNALLVVHYHRVVPYAPVKREQPKPAVKQDEQKPKRQASHWAVKPTAVGVHVPLPKKQEALEPAQSVPQQSLEEKAALTFGLFFSKGGGDESDAVILRKGKLFNRALNVPIDVKNTFVWAKIWDEASRRRGYFYVKDRAVKFFPIVRGRATIEDFIVNTAPGCDVALPRIELWSMRERAFVCTTKGWCWFNNERLRGEIYRRRCFSSSFSIGFLMHLGFRSLKVIRFAGTNILHMPSLNEERTFGWKGGDVYLPNVPKTAIVAGDRTRLG | ||||||
Chain | PRO_0000402531 | 1-2233 | Replicase protein 1a | |||
Sequence: MDYIRPLRVFSFPHVNNTLEYVRYNKANGDVGAFLTTMKFIGNVKLSDFTPRCAAMIYIGKLTKGVKRTFVPPPVKGFARQYAVVSGSVSALRGDGKKVLMEARTSTSATSDVSDFDVVFEAVSNALLVVHYHRVVPYAPVKREQPKPAVKQDEQKPKRQASHWAVKPTAVGVHVPLPKKQEALEPAQSVPQQSLEEKAALTFGLFFSKGGGDESDAVILRKGKLFNRALNVPIDVKNTFVWAKIWDEASRRRGYFYVKDRAVKFFPIVRGRATIEDFIVNTAPGCDVALPRIELWSMRERAFVCTTKGWCWFNNERLRGEIYRRRCFSSSFSIGFLMHLGFRSLKVIRFAGTNILHMPSLNEERTFGWKGGDVYLPNVPKTAIVAGDRTRLGGEILASVANALNQEEVYSSVVSSITNRLVLRDQSALLSHLDTKLCDMFSQRDAMIREKPSHRCDVFLKPREREKLRELFPELSIQFSDSVRSSHPFANAMRSCFNGIFSRRCGNVCFFDIGGSFTYHVKAGHVNCHVCNPVLDVKDVKRRINEILFLSTAGGDSYVSSDLLTEAASKSVSYCSRESQNCDSRADAGFMVDVYDISPQQVAEAMDKKGALVFDIALMFPVELLYGNGEVYLEELDTLVKREGDYLAYNVGQCGEMYEHSFSNVSGFFTFSYVRTSSGNVFKLEYEGYRCGYHHLTMCRAQKSPGTEVTYRSLVPSFVGKSLVFIPVVAGSSVSFKTIVLDSDFVDRIYSYALNTIGTFENRTFEYAVGAVRSQKTHVITGSRVVHSKVDISPDDMWGLVVAVMAQAIKDRAKSIRSYNFIKASEGSLAGVFKLFFQTVGDCFSNAVSVYAKAMVHDNFNVLETLMSMPRAFIRKVPGSVVVTICTSGASDRLELRGAFDISKETFGRKLKNSRLRVFSRAIVEDSIKVMKAMKTEDGKPLPITEDSVYAFIMGNVSNVHCTRAGLLGGSKATVVSSVSKGLVARGAATKAFSGITSFFSTGSLFYDRGLTEDERLDALVRTENAINSPVGILETSRVAVSKVVAGTKEFWSEVSLNDFTTFVLRNKVLIGIFVASLGAAPIAWKYRRGIAANARRYAGSSYETLSSLSSQAAGGLRGLTSSTVSGGSLVVRRGFSSAVTVTRATVAKRQVPLALLSFSTSYAISGCSMLGIWAHALPRHLMFFFGLGTLLGARASANTWKFGGFSNNWCAVPEVVWRGKSVSSLLLPITLGVSLIIRGLLNDTIPQLAYVPPVEGRNVYDETLRYYRDFDYDEGAGPSGTQHEAVPGDDNDGSTSSVSSYDVVTNVRDVGISTNGEVTGEEETHSPRSVQYTYVEEEVAPSAAVAERQGDPSGSGTADAMAFVESVKKGVDDVFHQQSSGETAREVEVDGKGLLPESVVGEAPTQERGRAADGNTAQTAVNEGDREPVQSSLVSSPQADIPKVTQSEVHAQKEVKQEVPLATVSGATPIVDEKPAPSVTTRGVKIIDKGKAVAHVAEKKQVQVEQPKQRSLTINEGKAGKQLCMFRTCSCGVQLDVYNEATIATRFSNAFTFVDNLKGRSAVFFSKLGEGYTYNGGSHVSSGWPRALEDILTAIKYPSVFDHCLVQKYKMGGGVPFHADDEECYPSDNPILTVNLVGKANFSTKCRKGGKVMVINVASGDYFLMPCGFQRTHLHSVNSIDEGRISLTFRATRRVFGVGRMLQLAGGVSDEKSPGVPNQQPQSQGATRTITPKSGGKALSEGSGREVKGRSTYSIWCEQDYVRKCEWLRADNPVMALEPDYTPMTFEVVKTGTSEDAVVEYLKYLAIGIERTYRALLMARNIAVTTAEGVLKVPNQVYESLPGFHVYKSGTDLIFHSTQDGLRVRDLPYVLIAEKGIFTKGKDVDAVVALGDNLFVCDDILVFHDAINLIGALKVARCGMVGESFKSFEYKCYNAPPGGGKTTTLVDEFVKSPNSTATITANVGSSEDINMAVKKRDPNLEGLNSATTVNSRVVNFIVRGMYKRVLVDEVHMMHQGLLQLGVFATGASEGLFFGDINQIPFINREKVFRMDCAVFVPKKESVVYTSKSYRCPLDVCYLLSSMTVRGTEKCYPEKVVSGKDKPVVRSLSKRPIGTTDDVAEINADVYLCMTQLEKSDMKRSLKGKGKETPVMTVHEAQGKTFSDVVLFRTKKADDSLFTKQPHILVGLSRHTRSLVYAALSSKLDDKVGTYISDASPQSVSDALLHTFAPAGC | ||||||
Chain | PRO_0000402533 | 394-2233 | Methyltransferase/helicase | |||
Sequence: GEILASVANALNQEEVYSSVVSSITNRLVLRDQSALLSHLDTKLCDMFSQRDAMIREKPSHRCDVFLKPREREKLRELFPELSIQFSDSVRSSHPFANAMRSCFNGIFSRRCGNVCFFDIGGSFTYHVKAGHVNCHVCNPVLDVKDVKRRINEILFLSTAGGDSYVSSDLLTEAASKSVSYCSRESQNCDSRADAGFMVDVYDISPQQVAEAMDKKGALVFDIALMFPVELLYGNGEVYLEELDTLVKREGDYLAYNVGQCGEMYEHSFSNVSGFFTFSYVRTSSGNVFKLEYEGYRCGYHHLTMCRAQKSPGTEVTYRSLVPSFVGKSLVFIPVVAGSSVSFKTIVLDSDFVDRIYSYALNTIGTFENRTFEYAVGAVRSQKTHVITGSRVVHSKVDISPDDMWGLVVAVMAQAIKDRAKSIRSYNFIKASEGSLAGVFKLFFQTVGDCFSNAVSVYAKAMVHDNFNVLETLMSMPRAFIRKVPGSVVVTICTSGASDRLELRGAFDISKETFGRKLKNSRLRVFSRAIVEDSIKVMKAMKTEDGKPLPITEDSVYAFIMGNVSNVHCTRAGLLGGSKATVVSSVSKGLVARGAATKAFSGITSFFSTGSLFYDRGLTEDERLDALVRTENAINSPVGILETSRVAVSKVVAGTKEFWSEVSLNDFTTFVLRNKVLIGIFVASLGAAPIAWKYRRGIAANARRYAGSSYETLSSLSSQAAGGLRGLTSSTVSGGSLVVRRGFSSAVTVTRATVAKRQVPLALLSFSTSYAISGCSMLGIWAHALPRHLMFFFGLGTLLGARASANTWKFGGFSNNWCAVPEVVWRGKSVSSLLLPITLGVSLIIRGLLNDTIPQLAYVPPVEGRNVYDETLRYYRDFDYDEGAGPSGTQHEAVPGDDNDGSTSSVSSYDVVTNVRDVGISTNGEVTGEEETHSPRSVQYTYVEEEVAPSAAVAERQGDPSGSGTADAMAFVESVKKGVDDVFHQQSSGETAREVEVDGKGLLPESVVGEAPTQERGRAADGNTAQTAVNEGDREPVQSSLVSSPQADIPKVTQSEVHAQKEVKQEVPLATVSGATPIVDEKPAPSVTTRGVKIIDKGKAVAHVAEKKQVQVEQPKQRSLTINEGKAGKQLCMFRTCSCGVQLDVYNEATIATRFSNAFTFVDNLKGRSAVFFSKLGEGYTYNGGSHVSSGWPRALEDILTAIKYPSVFDHCLVQKYKMGGGVPFHADDEECYPSDNPILTVNLVGKANFSTKCRKGGKVMVINVASGDYFLMPCGFQRTHLHSVNSIDEGRISLTFRATRRVFGVGRMLQLAGGVSDEKSPGVPNQQPQSQGATRTITPKSGGKALSEGSGREVKGRSTYSIWCEQDYVRKCEWLRADNPVMALEPDYTPMTFEVVKTGTSEDAVVEYLKYLAIGIERTYRALLMARNIAVTTAEGVLKVPNQVYESLPGFHVYKSGTDLIFHSTQDGLRVRDLPYVLIAEKGIFTKGKDVDAVVALGDNLFVCDDILVFHDAINLIGALKVARCGMVGESFKSFEYKCYNAPPGGGKTTTLVDEFVKSPNSTATITANVGSSEDINMAVKKRDPNLEGLNSATTVNSRVVNFIVRGMYKRVLVDEVHMMHQGLLQLGVFATGASEGLFFGDINQIPFINREKVFRMDCAVFVPKKESVVYTSKSYRCPLDVCYLLSSMTVRGTEKCYPEKVVSGKDKPVVRSLSKRPIGTTDDVAEINADVYLCMTQLEKSDMKRSLKGKGKETPVMTVHEAQGKTFSDVVLFRTKKADDSLFTKQPHILVGLSRHTRSLVYAALSSKLDDKVGTYISDASPQSVSDALLHTFAPAGC |
Family & Domains
Features
Showing features for region, compositional bias, domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 143-163 | Disordered | ||||
Sequence: REQPKPAVKQDEQKPKRQASH | ||||||
Compositional bias | 146-161 | Basic and acidic residues | ||||
Sequence: PKPAVKQDEQKPKRQA | ||||||
Domain | 478-669 | Alphavirus-like MT | ||||
Sequence: QFSDSVRSSHPFANAMRSCFNGIFSRRCGNVCFFDIGGSFTYHVKAGHVNCHVCNPVLDVKDVKRRINEILFLSTAGGDSYVSSDLLTEAASKSVSYCSRESQNCDSRADAGFMVDVYDISPQQVAEAMDKKGALVFDIALMFPVELLYGNGEVYLEELDTLVKREGDYLAYNVGQCGEMYEHSFSNVSGFF | ||||||
Region | 1277-1301 | Disordered | ||||
Sequence: AGPSGTQHEAVPGDDNDGSTSSVSS | ||||||
Region | 1400-1442 | Disordered | ||||
Sequence: VVGEAPTQERGRAADGNTAQTAVNEGDREPVQSSLVSSPQADI | ||||||
Compositional bias | 1412-1442 | Polar residues | ||||
Sequence: AADGNTAQTAVNEGDREPVQSSLVSSPQADI | ||||||
Domain | 1601-1694 | Fe2OG dioxygenase | ||||
Sequence: VFDHCLVQKYKMGGGVPFHADDEECYPSDNPILTVNLVGKANFSTKCRKGGKVMVINVASGDYFLMPCGFQRTHLHSVNSIDEGRISLTFRATR | ||||||
Region | 1708-1746 | Disordered | ||||
Sequence: GVSDEKSPGVPNQQPQSQGATRTITPKSGGKALSEGSGR | ||||||
Compositional bias | 1714-1737 | Polar residues | ||||
Sequence: SPGVPNQQPQSQGATRTITPKSGG | ||||||
Domain | 1902-2066 | +RNA virus helicase ATP-binding | ||||
Sequence: LVFHDAINLIGALKVARCGMVGESFKSFEYKCYNAPPGGGKTTTLVDEFVKSPNSTATITANVGSSEDINMAVKKRDPNLEGLNSATTVNSRVVNFIVRGMYKRVLVDEVHMMHQGLLQLGVFATGASEGLFFGDINQIPFINREKVFRMDCAVFVPKKESVVYT | ||||||
Domain | 2067-2230 | +RNA virus helicase C-terminal | ||||
Sequence: SKSYRCPLDVCYLLSSMTVRGTEKCYPEKVVSGKDKPVVRSLSKRPIGTTDDVAEINADVYLCMTQLEKSDMKRSLKGKGKETPVMTVHEAQGKTFSDVVLFRTKKADDSLFTKQPHILVGLSRHTRSLVYAALSSKLDDKVGTYISDASPQSVSDALLHTFAP |
Family and domain databases
Sequence & Isoform
- Sequence statusComplete
This entry describes 2 isoforms produced by Ribosomal frameshifting. The replicase 1a is produced from conventional translation of the 1a ORF. The replicase 1ab is generated probably by a +1 ribosomal frameshifting mechanism occurring at the 1a-1b genes boundary.
O71188-1
This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
- NameReplicase 1a
- Length2,233
- Mass (Da)244,804
- Last updated2003-06-01 v2
- ChecksumCDDC18B6133FB08C
O71189-1
The sequence of this isoform can be found in the external entry linked below. Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
View isoform- NameReplicase 1ab
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 146-161 | Basic and acidic residues | ||||
Sequence: PKPAVKQDEQKPKRQA | ||||||
Compositional bias | 1412-1442 | Polar residues | ||||
Sequence: AADGNTAQTAVNEGDREPVQSSLVSSPQADI | ||||||
Compositional bias | 1714-1737 | Polar residues | ||||
Sequence: SPGVPNQQPQSQGATRTITPKSGG |
Keywords
- Coding sequence diversity
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AF037268 EMBL· GenBank· DDBJ | AAC40717.3 EMBL· GenBank· DDBJ | Genomic RNA |