O70404 · VAMP8_MOUSE

  • Protein
    Vesicle-associated membrane protein 8
  • Gene
    Vamp8
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at protein level
  • Annotation score
    5/5

Function

function

SNAREs, soluble N-ethylmaleimide-sensitive factor-attachment protein receptors, are essential proteins for fusion of cellular membranes. SNAREs localized on opposing membranes assemble to form a trans-SNARE complex, an extended, parallel four alpha-helical bundle that drives membrane fusion. VAMP8 is a SNARE involved in autophagy through the direct control of autophagosome membrane fusion with the lysososome membrane via its interaction with the STX17-SNAP29 binary t-SNARE complex (By similarity).
Also required for dense-granule secretion in platelets (By similarity).
Also plays a role in regulated enzyme secretion in pancreatic acinar cells (PubMed:15363411).
Involved in the abscission of the midbody during cell division, which leads to completely separate daughter cells (By similarity).
Involved in the homotypic fusion of early and late endosomes (By similarity).
Participates also in the activation of type I interferon antiviral response through a TRIM6-dependent mechanism (By similarity).

Features

Showing features for site.

1101102030405060708090100
TypeIDPosition(s)Description
Site33Interaction with STX8

GO annotations

all annotationsall molecular functionvirus receptor activitydna bindingrna bindingcytoskeletal motor activitycatalytic activitygtpase activitystructural molecule activitytransporter activitycytoskeletal protein bindinglipid bindingcyclase activityantioxidant activityoxidoreductase activitytransferase activityhydrolase activitylyase activityisomerase activityligase activityprotein tag activitycargo receptor activityhistone bindingprotein folding chaperonetranslation regulator activitynutrient reservoir activityreceptor ligand activitymolecular transducer activitymolecular adaptor activitytoxin activitycell adhesion mediator activitymolecular function regulator activityvirus coreceptor activitycatalytic activity, acting on a proteincatalytic activity, acting on dnacatalytic activity, acting on rnamolecular carrier activitytranscription regulator activitygeneral transcription initiation factor activitymolecular sensor activitymolecular sequestering activityatp-dependent activityother molecular functionall biological processmitotic cell cyclecytokinesiscytoplasmic translationimmune system processmuscle system processcirculatory system processrenal system processrespiratory system processcarbohydrate metabolic processgeneration of precursor metabolites and energydna replicationdna repairdna recombinationchromatin organizationdna-templated transcriptionregulation of dna-templated transcriptiontrna metabolic processprotein foldingprotein glycosylationamino acid metabolic processmodified amino acid metabolic processlipid metabolic processvitamin metabolic processsulfur compound metabolic processintracellular protein transportnucleocytoplasmic transportautophagyinflammatory responsemitochondrion organizationcytoskeleton organizationmicrotubule-based movementperoxisome organizationlysosome organizationchromosome segregationcell adhesionestablishment or maintenance of cell polarityprogrammed cell deathphotosynthesismrna metabolic processsnrna metabolic processvesicle-mediated transportreproductive processdigestive system processsignalingcell differentiationprotein catabolic processextracellular matrix organizationregulatory ncrna-mediated gene silencingtelomere organizationcell junction organizationwound healingribosome biogenesiscilium organizationanatomical structure developmentcell motilitynervous system processendocrine processprotein maturationtransmembrane transportnucleobase-containing small molecule metabolic processhepaticobiliary system processmembrane organizationprotein-containing complex assemblycell wall organization or biogenesisnitrogen cycle metabolic processprotein localization to plasma membranedefense response to other organismdetoxificationmeiotic nuclear divisionmitotic nuclear divisionmitochondrial gene expressioncarbohydrate derivative metabolic processother biological processall cellular componentnuclear chromosomeextracellular regionextracellular spacecell wallnucleusnuclear envelopenucleoplasmchromosomenucleolusmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuslipid dropletmicrotubule organizing centercytosolribosomecytoskeletonplasma membraneciliumplastidthylakoidexternal encapsulating structureextracellular matrixcytoplasmic vesicleorganelleother cellular component
Cell color indicative of number of GO terms
AspectTerm
Cellular Componentbasal part of cell
Cellular Componentbasolateral part of cell
Cellular Componentbasolateral plasma membrane
Cellular Componentcell surface
Cellular Componentcytosol
Cellular Componentearly endosome
Cellular Componentearly endosome membrane
Cellular Componentlate endosome membrane
Cellular Componentlysosomal membrane
Cellular Componentlysosome
Cellular Componentmidbody
Cellular Componentmitochondrion
Cellular Componentmucin granule
Cellular Componentphagocytic vesicle
Cellular Componentplasma membrane
Cellular Componentrecycling endosome
Cellular Componentsecretory granule
Cellular ComponentSNARE complex
Cellular Componentzymogen granule
Cellular Componentzymogen granule membrane
Molecular FunctionSNAP receptor activity
Molecular Functionsyntaxin binding
Biological Processautophagosome maturation
Biological Processcellular response to type II interferon
Biological Processdefense response to virus
Biological Processestablishment of localization in cell
Biological Processmembrane fusion
Biological Processmucus secretion
Biological Processpositive regulation of pancreatic amylase secretion
Biological Processprotein transport
Biological Processprotein-containing complex assembly
Biological Processregulation of endocytosis
Biological ProcessSNARE complex assembly
Biological Processsymbiont entry into host cell
Biological Processvesicle fusion
Biological Processvesicle-mediated transport
Biological Processzymogen granule exocytosis

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Vesicle-associated membrane protein 8
  • Short names
    VAMP-8
  • Alternative names
    • Endobrevin
      (Edb
      )

Gene names

    • Name
      Vamp8

Organism names

  • Taxonomic identifier
  • Strains
    • C57BL/6J
    • FVB/N
  • Taxonomic lineage
    Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Mus > Mus

Accessions

  • Primary accession
    O70404
  • Secondary accessions
    • Q9CRA3

Proteomes

Organism-specific databases

Subcellular Location

Lysosome membrane
; Single-pass type IV membrane protein
Late endosome membrane
; Single-pass type IV membrane protein
Early endosome membrane
; Single-pass type IV membrane protein
Cell membrane
; Single-pass type IV membrane protein
Zymogen granule membrane
; Single-pass type IV membrane protein
Note: Perinuclear vesicular structures of the early and late endosomes, coated pits, and trans-Golgi (By similarity).
Sub-tight junctional domain in retinal pigment epithelium cells (By similarity).
Midbody region during cytokinesis (By similarity).
Lumenal oriented, apical membranes of nephric tubular cell (By similarity).
Cycles through the apical but not through the basolateral plasma membrane (By similarity).
Apical region of acinar cells; in zymogen granule membranes (PubMed:15363411).

Features

Showing features for topological domain, transmembrane.

TypeIDPosition(s)Description
Topological domain1-75Cytoplasmic
Transmembrane76-96Helical; Anchor for type IV membrane protein
Topological domain97-101Vesicular

Keywords

Phenotypes & Variants

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis24Blocks internalization from cell surface; when associated with A-27.
Mutagenesis27Blocks internalization from cell surface; when associated with A-24.

PTM/Processing

Features

Showing features for modified residue, chain.

TypeIDPosition(s)Description
Modified residue1N-acetylmethionine
ChainPRO_00002067371-101Vesicle-associated membrane protein 8
Modified residue5Phosphoserine
Modified residue18Phosphoserine
Modified residue28Phosphothreonine
Modified residue48Phosphothreonine
Modified residue54Phosphothreonine
Modified residue55Phosphoserine

Keywords

Proteomic databases

PTM databases

Expression

Tissue specificity

Expressed abundantly in the kidney, less in the liver, brain, kidney, heart, lung, pancreas and placenta.

Interaction

Subunit

Forms a SNARE complex composed of VAMP8, SNAP29 and STX17 involved in fusion of autophagosome with lysosome (By similarity).
Found in a number of SNARE complexes with NAPA, SNAP23, SNAP25, STX1A, STX4, STX7, STX8 and VTI1B (PubMed:15363411).
Interacts with PICALM (PubMed:22118466).
SNARE complex formation and binding by PICALM are mutually exclusive processes for VAMP8 (PubMed:22118466).
Interacts with SBF2/MTMR13 (By similarity).
Interacts with RAB21 (in GTP-bound form) in response to starvation; the interaction probably regulates VAMP8 endolysosomal trafficking (By similarity).
Interacts with STX17; this interaction is increased in the absence of TMEM39A (By similarity).
Interacts with TRIM6 (By similarity).

Binary interactions

TypeEntry 1Entry 2Number of experimentsIntact
XENO O70404Picalm O550124EBI-1812572, EBI-915601

Protein-protein interaction databases

Miscellaneous

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain12-72v-SNARE coiled-coil homology

Sequence similarities

Belongs to the synaptobrevin family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    101
  • Mass (Da)
    11,451
  • Last updated
    1998-08-01 v1
  • Checksum
    0A2FDB6B69E06C3E
MEEASGSAGNDRVRNLQSEVEGVKNIMTQNVERILSRGENLDHLRNKTEDLEATSEHFKTTSQKVARKFWWKNVKMIVIICVIVLIIVILIILFATGTIPT

Computationally mapped potential isoform sequences

There are 2 potential isoforms mapped to this entry

View all
EntryEntry nameGene nameLength
A0A0R4J0R1A0A0R4J0R1_MOUSEVamp8101
A0A0U1RPE8A0A0U1RPE8_MOUSEVamp875

Features

Showing features for sequence conflict.

TypeIDPosition(s)Description
Sequence conflict36in Ref. 4 and 5

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AF053724
EMBL· GenBank· DDBJ
AAC06371.1
EMBL· GenBank· DDBJ
mRNA
AF045661
EMBL· GenBank· DDBJ
AAC23665.1
EMBL· GenBank· DDBJ
mRNA
AF247556
EMBL· GenBank· DDBJ
AAK48423.1
EMBL· GenBank· DDBJ
Genomic DNA
AK002268
EMBL· GenBank· DDBJ
BAB21977.1
EMBL· GenBank· DDBJ
mRNA
AK013279
EMBL· GenBank· DDBJ
BAB28766.1
EMBL· GenBank· DDBJ
mRNA
BC012668
EMBL· GenBank· DDBJ
AAH12668.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

Disclaimer

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