O70404 · VAMP8_MOUSE
- ProteinVesicle-associated membrane protein 8
- GeneVamp8
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids101 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
SNAREs, soluble N-ethylmaleimide-sensitive factor-attachment protein receptors, are essential proteins for fusion of cellular membranes. SNAREs localized on opposing membranes assemble to form a trans-SNARE complex, an extended, parallel four alpha-helical bundle that drives membrane fusion. VAMP8 is a SNARE involved in autophagy through the direct control of autophagosome membrane fusion with the lysososome membrane via its interaction with the STX17-SNAP29 binary t-SNARE complex (By similarity).
Also required for dense-granule secretion in platelets (By similarity).
Also plays a role in regulated enzyme secretion in pancreatic acinar cells (PubMed:15363411).
Involved in the abscission of the midbody during cell division, which leads to completely separate daughter cells (By similarity).
Involved in the homotypic fusion of early and late endosomes (By similarity).
Participates also in the activation of type I interferon antiviral response through a TRIM6-dependent mechanism (By similarity).
Also required for dense-granule secretion in platelets (By similarity).
Also plays a role in regulated enzyme secretion in pancreatic acinar cells (PubMed:15363411).
Involved in the abscission of the midbody during cell division, which leads to completely separate daughter cells (By similarity).
Involved in the homotypic fusion of early and late endosomes (By similarity).
Participates also in the activation of type I interferon antiviral response through a TRIM6-dependent mechanism (By similarity).
Features
Showing features for site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Site | 33 | Interaction with STX8 | ||||
Sequence: R |
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Keywords
- Biological process
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameVesicle-associated membrane protein 8
- Short namesVAMP-8
- Alternative names
Gene names
Organism names
- Organism
- Strains
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Mus > Mus
Accessions
- Primary accessionO70404
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Lysosome membrane ; Single-pass type IV membrane protein
Late endosome membrane ; Single-pass type IV membrane protein
Early endosome membrane ; Single-pass type IV membrane protein
Cell membrane ; Single-pass type IV membrane protein
Zymogen granule membrane ; Single-pass type IV membrane protein
Note: Perinuclear vesicular structures of the early and late endosomes, coated pits, and trans-Golgi (By similarity).
Sub-tight junctional domain in retinal pigment epithelium cells (By similarity).
Midbody region during cytokinesis (By similarity).
Lumenal oriented, apical membranes of nephric tubular cell (By similarity).
Cycles through the apical but not through the basolateral plasma membrane (By similarity).
Apical region of acinar cells; in zymogen granule membranes (PubMed:15363411).
Sub-tight junctional domain in retinal pigment epithelium cells (By similarity).
Midbody region during cytokinesis (By similarity).
Lumenal oriented, apical membranes of nephric tubular cell (By similarity).
Cycles through the apical but not through the basolateral plasma membrane (By similarity).
Apical region of acinar cells; in zymogen granule membranes (PubMed:15363411).
Features
Showing features for topological domain, transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Topological domain | 1-75 | Cytoplasmic | ||||
Sequence: MEEASGSAGNDRVRNLQSEVEGVKNIMTQNVERILSRGENLDHLRNKTEDLEATSEHFKTTSQKVARKFWWKNVK | ||||||
Transmembrane | 76-96 | Helical; Anchor for type IV membrane protein | ||||
Sequence: MIVIICVIVLIIVILIILFAT | ||||||
Topological domain | 97-101 | Vesicular | ||||
Sequence: GTIPT |
Keywords
- Cellular component
Phenotypes & Variants
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 24 | Blocks internalization from cell surface; when associated with A-27. | ||||
Sequence: K → A | ||||||
Mutagenesis | 27 | Blocks internalization from cell surface; when associated with A-24. | ||||
Sequence: M → A |
PTM/Processing
Features
Showing features for modified residue, chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Modified residue | 1 | N-acetylmethionine | ||||
Sequence: M | ||||||
Chain | PRO_0000206737 | 1-101 | Vesicle-associated membrane protein 8 | |||
Sequence: MEEASGSAGNDRVRNLQSEVEGVKNIMTQNVERILSRGENLDHLRNKTEDLEATSEHFKTTSQKVARKFWWKNVKMIVIICVIVLIIVILIILFATGTIPT | ||||||
Modified residue | 5 | Phosphoserine | ||||
Sequence: S | ||||||
Modified residue | 18 | Phosphoserine | ||||
Sequence: S | ||||||
Modified residue | 28 | Phosphothreonine | ||||
Sequence: T | ||||||
Modified residue | 48 | Phosphothreonine | ||||
Sequence: T | ||||||
Modified residue | 54 | Phosphothreonine | ||||
Sequence: T | ||||||
Modified residue | 55 | Phosphoserine | ||||
Sequence: S |
Keywords
- PTM
Proteomic databases
PTM databases
Expression
Tissue specificity
Expressed abundantly in the kidney, less in the liver, brain, kidney, heart, lung, pancreas and placenta.
Interaction
Subunit
Forms a SNARE complex composed of VAMP8, SNAP29 and STX17 involved in fusion of autophagosome with lysosome (By similarity).
Found in a number of SNARE complexes with NAPA, SNAP23, SNAP25, STX1A, STX4, STX7, STX8 and VTI1B (PubMed:15363411).
Interacts with PICALM (PubMed:22118466).
SNARE complex formation and binding by PICALM are mutually exclusive processes for VAMP8 (PubMed:22118466).
Interacts with SBF2/MTMR13 (By similarity).
Interacts with RAB21 (in GTP-bound form) in response to starvation; the interaction probably regulates VAMP8 endolysosomal trafficking (By similarity).
Interacts with STX17; this interaction is increased in the absence of TMEM39A (By similarity).
Interacts with TRIM6 (By similarity).
Found in a number of SNARE complexes with NAPA, SNAP23, SNAP25, STX1A, STX4, STX7, STX8 and VTI1B (PubMed:15363411).
Interacts with PICALM (PubMed:22118466).
SNARE complex formation and binding by PICALM are mutually exclusive processes for VAMP8 (PubMed:22118466).
Interacts with SBF2/MTMR13 (By similarity).
Interacts with RAB21 (in GTP-bound form) in response to starvation; the interaction probably regulates VAMP8 endolysosomal trafficking (By similarity).
Interacts with STX17; this interaction is increased in the absence of TMEM39A (By similarity).
Interacts with TRIM6 (By similarity).
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | Intact | |
---|---|---|---|---|---|
XENO | O70404 | Picalm O55012 | 4 | EBI-1812572, EBI-915601 |
Protein-protein interaction databases
Miscellaneous
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 12-72 | v-SNARE coiled-coil homology | ||||
Sequence: RVRNLQSEVEGVKNIMTQNVERILSRGENLDHLRNKTEDLEATSEHFKTTSQKVARKFWWK |
Sequence similarities
Belongs to the synaptobrevin family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length101
- Mass (Da)11,451
- Last updated1998-08-01 v1
- Checksum0A2FDB6B69E06C3E
Computationally mapped potential isoform sequences
There are 2 potential isoforms mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
A0A0R4J0R1 | A0A0R4J0R1_MOUSE | Vamp8 | 101 | ||
A0A0U1RPE8 | A0A0U1RPE8_MOUSE | Vamp8 | 75 |
Features
Showing features for sequence conflict.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Sequence conflict | 36 | in Ref. 4 and 5 | ||||
Sequence: S → A |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AF053724 EMBL· GenBank· DDBJ | AAC06371.1 EMBL· GenBank· DDBJ | mRNA | ||
AF045661 EMBL· GenBank· DDBJ | AAC23665.1 EMBL· GenBank· DDBJ | mRNA | ||
AF247556 EMBL· GenBank· DDBJ | AAK48423.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AK002268 EMBL· GenBank· DDBJ | BAB21977.1 EMBL· GenBank· DDBJ | mRNA | ||
AK013279 EMBL· GenBank· DDBJ | BAB28766.1 EMBL· GenBank· DDBJ | mRNA | ||
BC012668 EMBL· GenBank· DDBJ | AAH12668.1 EMBL· GenBank· DDBJ | mRNA |