O69250 · PTHP_PRIMG
- ProteinPhosphocarrier protein HPr
- GeneptsH
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids
- Protein existenceEvidence at protein level
- Annotation score3/5
Function
function
General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. The phosphoryl group from phosphoenolpyruvate (PEP) is transferred to the phosphoryl carrier protein HPr by enzyme I. Phospho-HPr then transfers it to the PTS EIIA domain.
P-Ser-HPr interacts with the catabolite control protein A (CcpA), forming a complex that binds to DNA at the catabolite response elements cre, operator sites preceding a large number of catabolite-regulated genes. Thus, P-Ser-HPr is a corepressor in carbon catabolite repression (CCR), a mechanism that allows bacteria to coordinate and optimize the utilization of available carbon sources. P-Ser-HPr also plays a role in inducer exclusion, in which it probably interacts with several non-PTS permeases and inhibits their transport activity (By similarity).
Activity regulation
Phosphorylation on Ser-46 inhibits the phosphoryl transfer from enzyme I to HPr.
Features
Showing features for active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Active site | 15 | Pros-phosphohistidine intermediate | ||||
Sequence: H |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Biological Process | phosphoenolpyruvate-dependent sugar phosphotransferase system |
Keywords
- Biological process
Names & Taxonomy
Protein names
- Recommended namePhosphocarrier protein HPr
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Bacillota > Bacilli > Bacillales > Bacillaceae > Priestia
Accessions
- Primary accessionO69250
Subcellular Location
PTM/Processing
Features
Showing features for chain, modified residue.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000107839 | 1-88 | Phosphocarrier protein HPr | |||
Sequence: MAQKTFTVTADSGIHARPATTLVQAASKFDSDINLEFNGKTVNLKSIMGVMSLGIQKGATITISAEGSDEADALAALEDTMSKEGLGE | ||||||
Modified residue | 12 | Phosphoserine | ||||
Sequence: S | ||||||
Modified residue | 46 | Phosphoserine; by HPrK/P | ||||
Sequence: S |
Keywords
- PTM
PTM databases
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 1-88 | HPr | ||||
Sequence: MAQKTFTVTADSGIHARPATTLVQAASKFDSDINLEFNGKTVNLKSIMGVMSLGIQKGATITISAEGSDEADALAALEDTMSKEGLGE |
Sequence similarities
Belongs to the HPr family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length88
- Mass (Da)9,119
- Last updated1998-08-01 v1
- Checksum4EDC85C2E0B9AA3B
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AJ005075 EMBL· GenBank· DDBJ | CAA06331.1 EMBL· GenBank· DDBJ | Genomic DNA |