O66164 · FTHS_SPHSK
- ProteinFormate--tetrahydrofolate ligase
- Genefhs
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids558 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
Catalytic activity
- (6S)-5,6,7,8-tetrahydrofolate + ATP + formate = (6R)-10-formyltetrahydrofolate + ADP + phosphate
Pathway
One-carbon metabolism; tetrahydrofolate interconversion.
Features
Showing features for binding site.
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | ATP binding | |
Molecular Function | formate-tetrahydrofolate ligase activity | |
Biological Process | tetrahydrofolate interconversion |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameFormate--tetrahydrofolate ligase
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Alphaproteobacteria > Sphingomonadales > Sphingomonadaceae > Sphingobium
Accessions
- Primary accessionO66164
- Secondary accessions
Proteomes
Phenotypes & Variants
Disruption phenotype
Mutant cannot degrade vanillate or syringate, but it can degrade protocatechuate and 3-O-methylgallate (PubMed:9501423).
Mutant shows O-demethylation activity toward both vanillate and syringate in the presence of tetrahydrofolate, indicating that ligH is not directly involved in the O demethylation of vanillate and syringate (PubMed:15090517).
Mutant shows O-demethylation activity toward both vanillate and syringate in the presence of tetrahydrofolate, indicating that ligH is not directly involved in the O demethylation of vanillate and syringate (PubMed:15090517).
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000199370 | 1-558 | Formate--tetrahydrofolate ligase | |||
Sequence: MAQGSDIEIAREAKMQNIAEVGAKVGIPQDALLNYGPYKAKLSWDFINSVQGNQDGKLILVTAINPTPAGEGKTTTTVGLADGLNRIGKKTVAALREPSLGPCFGVKGGAAGGGYAQVVPMEDINLHFTGDFHAITSANNLLAALIDNHIYWGNKLGLDPRRIAWRRVLDMNDRALRSIVNSLGGVSNGYPREDGFDITVASEVMAILCLSSDLKDLERRLGNIHAGYTRERKAVLASELNASGAMTVLLKDALQPNMVQTLENNPVLIHGGPFANIAHGCNSVLATKTALKIADYVVTEAGFGADLGAEKFFDIKCRKAGLKPSAAVIVATIRALKMHGGVDKADLGTANPEAVRKGGVNLARHIENVRQFGVPVVVAINQFITDTDEEMAMVKEIAEAAGAEAVLCSHWANGSAGTEELARKVVLHAESGSSNFAPLYEDSMPLFEKIDTIAKRIYRATEATADSSVRNKLKGWEADGFGHLPVCMAKTQYSFSTDPALRGAPTDHVVPVRDVILSAGAEFIVAVCGDIMRMPGLPKVPSADFIKLDEQGQIQGLF |
Interaction
Protein-protein interaction databases
Structure
Sequence
- Sequence statusComplete
- Length558
- Mass (Da)59,464
- Last updated2019-05-08 v2
- Checksum1DEA902749928A65
Features
Showing features for sequence conflict.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Sequence conflict | 13-14 | in Ref. 1; BAA25140 | ||||
Sequence: AK → ER | ||||||
Sequence conflict | 175-176 | in Ref. 1; BAA25140 | ||||
Sequence: AL → V | ||||||
Sequence conflict | 427 | in Ref. 1; BAA25140 | ||||
Sequence: L → H |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AB006079 EMBL· GenBank· DDBJ | BAA25140.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AB186750 EMBL· GenBank· DDBJ | BAD61061.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AP012222 EMBL· GenBank· DDBJ | BAK65951.1 EMBL· GenBank· DDBJ | Genomic DNA |